Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori
Objective(s): Recently, magnetic nanoparticles coated with different ligands have been utilized in diagnosis, drug delivery, and therapy. This study aimed to synthesize, characterize, and apply Fe3O4 coated with Poly Dopamine (PDA) as an MR imaging nanoprobe and its application in metronidazole-res...
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| Format: | Article |
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Mashhad University of Medical Sciences
2025-04-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_25607_3ad0879cfaee7fd8a895e15a86612085.pdf |
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| author | Mohammad Ghaderian Mohammad Reza Salamat Daryoush Shahbazi-Gahrouei Fahimeh Hossein Beigi Amir Sajedi Gholamreza Amiri Tahmineh Narimani |
| author_facet | Mohammad Ghaderian Mohammad Reza Salamat Daryoush Shahbazi-Gahrouei Fahimeh Hossein Beigi Amir Sajedi Gholamreza Amiri Tahmineh Narimani |
| author_sort | Mohammad Ghaderian |
| collection | DOAJ |
| description | Objective(s): Recently, magnetic nanoparticles coated with different ligands have been utilized in diagnosis, drug delivery, and therapy. This study aimed to synthesize, characterize, and apply Fe3O4 coated with Poly Dopamine (PDA) as an MR imaging nanoprobe and its application in metronidazole-resistant helicobacter pylori (H.pylori).Materials and Methods: Fe3O4 nanoparticles were characterized and their cytotoxicity was determined in MCF-7 cell lines. Then, coated and non-coated nanoparticles were injected intravenously into 9 xenograft BALB/C mice, and the signal intensity of the T2-weighted MR was assessed. Iron concentrations were measured by ICP-AES, and histopathological assessment was done on harvested critical organs. Finally, The standard disk diffusion method was also used to identify the H.pylori resistance to metronidazole. The minimum inhibitory concentrations and the minimum bactericidal concentration of uncoated and coated iron oxide nanoparticles were investigated.Results: The MTT assay showed low cytotoxicity at 512 µg/ml. T2 relaxation times of tumors were lower compared to normal tissues. ICP-AES results indicated that the NPs accumulated mostly in the spleen and liver. The histopathology study demonstrated that the vital organ tissues had less morphologic abnormality and apoptotic changes. The minimum inhibitory and minimum bactericidal concentrations of ION@PDA were 16-64 µg/ml and 16-128 µg/ml, respectively. Conclusion: Due to the high permeability of nanoprobes in tumors, less cytotoxicity, and its inhibitory effects on metronidazole-resistant H.pylori, ION@PDA may be used as a nano contrast agent in MR imaging for the detection of MCF-7 cells. |
| format | Article |
| id | doaj-art-821f617f9fdd4d13af108ca0111dc7eb |
| institution | Kabale University |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-821f617f9fdd4d13af108ca0111dc7eb2025-08-20T03:58:41ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-04-0112220221510.22038/nmj.2025.77014.187225607Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter PyloriMohammad Ghaderian0Mohammad Reza Salamat1Daryoush Shahbazi-Gahrouei2Fahimeh Hossein Beigi3Amir Sajedi4Gholamreza Amiri5Tahmineh Narimani6Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Biophysics Falavarjan Branch, Islamic Azad University, Isfahan, IranDepartment of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranFalavarjan Branch, Islamic Azad University, Isfahan, IranDepartment of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranObjective(s): Recently, magnetic nanoparticles coated with different ligands have been utilized in diagnosis, drug delivery, and therapy. This study aimed to synthesize, characterize, and apply Fe3O4 coated with Poly Dopamine (PDA) as an MR imaging nanoprobe and its application in metronidazole-resistant helicobacter pylori (H.pylori).Materials and Methods: Fe3O4 nanoparticles were characterized and their cytotoxicity was determined in MCF-7 cell lines. Then, coated and non-coated nanoparticles were injected intravenously into 9 xenograft BALB/C mice, and the signal intensity of the T2-weighted MR was assessed. Iron concentrations were measured by ICP-AES, and histopathological assessment was done on harvested critical organs. Finally, The standard disk diffusion method was also used to identify the H.pylori resistance to metronidazole. The minimum inhibitory concentrations and the minimum bactericidal concentration of uncoated and coated iron oxide nanoparticles were investigated.Results: The MTT assay showed low cytotoxicity at 512 µg/ml. T2 relaxation times of tumors were lower compared to normal tissues. ICP-AES results indicated that the NPs accumulated mostly in the spleen and liver. The histopathology study demonstrated that the vital organ tissues had less morphologic abnormality and apoptotic changes. The minimum inhibitory and minimum bactericidal concentrations of ION@PDA were 16-64 µg/ml and 16-128 µg/ml, respectively. Conclusion: Due to the high permeability of nanoprobes in tumors, less cytotoxicity, and its inhibitory effects on metronidazole-resistant H.pylori, ION@PDA may be used as a nano contrast agent in MR imaging for the detection of MCF-7 cells.https://nmj.mums.ac.ir/article_25607_3ad0879cfaee7fd8a895e15a86612085.pdfmagnetic iron oxide nanoparticlesbreast cancermagnetic resonance imaginghelicobacter pylori |
| spellingShingle | Mohammad Ghaderian Mohammad Reza Salamat Daryoush Shahbazi-Gahrouei Fahimeh Hossein Beigi Amir Sajedi Gholamreza Amiri Tahmineh Narimani Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori Nanomedicine Journal magnetic iron oxide nanoparticles breast cancer magnetic resonance imaging helicobacter pylori |
| title | Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori |
| title_full | Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori |
| title_fullStr | Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori |
| title_full_unstemmed | Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori |
| title_short | Fe3O4@Poly-dopamine as T2W MR Nanoprobe for breast cancer detection and as inhibitory Agent in Helicobacter Pylori |
| title_sort | fe3o4 poly dopamine as t2w mr nanoprobe for breast cancer detection and as inhibitory agent in helicobacter pylori |
| topic | magnetic iron oxide nanoparticles breast cancer magnetic resonance imaging helicobacter pylori |
| url | https://nmj.mums.ac.ir/article_25607_3ad0879cfaee7fd8a895e15a86612085.pdf |
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