Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results

The gut microbiota plays a basic role in maintaining the psychophysical health and well-being of horses. Understanding the complex interactions among microbial communities in relation to age, management, and health is a topic of growing interest. The equine microbiota, given its complexity, is subje...

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Main Authors: Maria Claudia Curadi, Flavio Vallone, Martina Tenuzzo, Angelo Gazzano, Valentina Gazzano, Fabio Macchioni, Claudia Vannini
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Veterinary Sciences
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Online Access:https://www.mdpi.com/2306-7381/12/4/309
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author Maria Claudia Curadi
Flavio Vallone
Martina Tenuzzo
Angelo Gazzano
Valentina Gazzano
Fabio Macchioni
Claudia Vannini
author_facet Maria Claudia Curadi
Flavio Vallone
Martina Tenuzzo
Angelo Gazzano
Valentina Gazzano
Fabio Macchioni
Claudia Vannini
author_sort Maria Claudia Curadi
collection DOAJ
description The gut microbiota plays a basic role in maintaining the psychophysical health and well-being of horses. Understanding the complex interactions among microbial communities in relation to age, management, and health is a topic of growing interest. The equine microbiota, given its complexity, is subject to variations caused by internal or external stressors that can lead to metabolic problems and pathologies, i.e., obesity and laminitis. The aim of this study was to characterize the fecal microbiota of 12 purebred Arabian horses and to compare the microbial communities in two distinct management systems (Group 1 = box 22 h/day + paddock 2 h/day and Group 2 = paddock 24 h). Fecal samples were analyzed using high-throughput sequencing of 16S rRNA V3-V4 amplicons. The microbiota was predominantly composed of Firmicutes (32–53%) and Bacteroidetes (32–47.8%), with Ruminococcaceae emerging as the most prevalent bacterial family (10.9–24%). Comparisons of alpha and beta diversity revealed no statistically significant differences between the groups based on different management systems. This preliminary characterization contributes valuable data for understanding the equine fecal microbiota and lays the groundwork for future studies on the relationship among intestinal microbiota and equine health, performance, and management strategies.
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spelling doaj-art-821d70c3b0714e0f92ab401b4470c9212025-08-20T03:13:45ZengMDPI AGVeterinary Sciences2306-73812025-03-0112430910.3390/vetsci12040309Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary ResultsMaria Claudia Curadi0Flavio Vallone1Martina Tenuzzo2Angelo Gazzano3Valentina Gazzano4Fabio Macchioni5Claudia Vannini6Department of Veterinary Sciences, University of Pisa, 56126 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56126 Pisa, ItalyDepartment of Biology, Via Volta 4, 56126 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56126 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56126 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56126 Pisa, ItalyDepartment of Biology, Via Volta 4, 56126 Pisa, ItalyThe gut microbiota plays a basic role in maintaining the psychophysical health and well-being of horses. Understanding the complex interactions among microbial communities in relation to age, management, and health is a topic of growing interest. The equine microbiota, given its complexity, is subject to variations caused by internal or external stressors that can lead to metabolic problems and pathologies, i.e., obesity and laminitis. The aim of this study was to characterize the fecal microbiota of 12 purebred Arabian horses and to compare the microbial communities in two distinct management systems (Group 1 = box 22 h/day + paddock 2 h/day and Group 2 = paddock 24 h). Fecal samples were analyzed using high-throughput sequencing of 16S rRNA V3-V4 amplicons. The microbiota was predominantly composed of Firmicutes (32–53%) and Bacteroidetes (32–47.8%), with Ruminococcaceae emerging as the most prevalent bacterial family (10.9–24%). Comparisons of alpha and beta diversity revealed no statistically significant differences between the groups based on different management systems. This preliminary characterization contributes valuable data for understanding the equine fecal microbiota and lays the groundwork for future studies on the relationship among intestinal microbiota and equine health, performance, and management strategies.https://www.mdpi.com/2306-7381/12/4/309Arabian horseequinefecal microbiotaGIT microbiotamicrobiomemetabarcoding
spellingShingle Maria Claudia Curadi
Flavio Vallone
Martina Tenuzzo
Angelo Gazzano
Valentina Gazzano
Fabio Macchioni
Claudia Vannini
Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results
Veterinary Sciences
Arabian horse
equine
fecal microbiota
GIT microbiota
microbiome
metabarcoding
title Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results
title_full Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results
title_fullStr Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results
title_full_unstemmed Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results
title_short Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results
title_sort effect of management system on fecal microbiota in arabian horses preliminary results
topic Arabian horse
equine
fecal microbiota
GIT microbiota
microbiome
metabarcoding
url https://www.mdpi.com/2306-7381/12/4/309
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AT angelogazzano effectofmanagementsystemonfecalmicrobiotainarabianhorsespreliminaryresults
AT valentinagazzano effectofmanagementsystemonfecalmicrobiotainarabianhorsespreliminaryresults
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