Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study
The current study demonstrates the manufacturing of highly sensitive aptasensr for the robust and effective detection of dengue virus antigen. The proposed electrochemical aptasensor employs both types of electrodes, namely commercialized screen-printed electrodes (C-SPEs) and self-fabricated screen...
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KeAi Communications Co., Ltd.
2024-01-01
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| Series: | Sensors International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266635112400007X |
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| author | Mohd Rahil Hasan Pradakshina Sharma Saumitra Singh Sushil Kumar Shivani Kirti Rani Jagriti Narang |
| author_facet | Mohd Rahil Hasan Pradakshina Sharma Saumitra Singh Sushil Kumar Shivani Kirti Rani Jagriti Narang |
| author_sort | Mohd Rahil Hasan |
| collection | DOAJ |
| description | The current study demonstrates the manufacturing of highly sensitive aptasensr for the robust and effective detection of dengue virus antigen. The proposed electrochemical aptasensor employs both types of electrodes, namely commercialized screen-printed electrodes (C-SPEs) and self-fabricated screen-printed electrodes (SF-SPEs), were efficiently diagnose dengue virus antigen (DENV-Ag) and shows a lower limit of detection (LOD) i.e., 0.1 μg/ml. Both the electrode types were coated with chemically synthesized ZnO-Nanomaterial, which aids in electron transport, and to make it more selective highly specific DNA-aptamer was used against the DENV antigen. SEM and Uv–Vis spectra approaches were used to characterize the synthesized nanomaterial. To confirm the DENV-antigen detection results, electrochemical analysis was performed and the sensor cross-reactivity was also checked by a close member of the dengue virus i.e., chikungunya virus (CHIKV). The developed platform based on SF-SPEs & C-SPEs performed well in human serum. This investigation found that the SF-SPEs system had advanced sensitivity and responded very well to the C-SPEs. Consequently, the SF-SPEs system has emerged as a feasible choice for low-cost and highly sensitive DENV-detection and is also applicable for other analytes diagnostics. |
| format | Article |
| id | doaj-art-8210300bb2b44f3eb06e4fa545f04a84 |
| institution | Kabale University |
| issn | 2666-3511 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Sensors International |
| spelling | doaj-art-8210300bb2b44f3eb06e4fa545f04a842025-01-04T04:57:09ZengKeAi Communications Co., Ltd.Sensors International2666-35112024-01-015100285Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative studyMohd Rahil Hasan0Pradakshina Sharma1Saumitra Singh2Sushil Kumar3 Shivani4Kirti Rani5Jagriti Narang6Department of Biotechnology, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Biotechnology, Jamia Hamdard, New Delhi, 110062, IndiaDepartment of Biotechnology, Jamia Hamdard, New Delhi, 110062, IndiaIndian Institute of Technology Guwahati, Amingaon, North Guwahati, Guwahati, Assam, 781039, IndiaKurukshetra University. Kurukshetra, Thanesar, Haryana, 136119, IndiaAmity Institute of Biotechnology, Amity University Uttar Pradesh Noida (Uttar Pradesh), Gautam Buddha Nagar, 201303, IndiaDepartment of Biotechnology, Jamia Hamdard, New Delhi, 110062, India; Corresponding author.The current study demonstrates the manufacturing of highly sensitive aptasensr for the robust and effective detection of dengue virus antigen. The proposed electrochemical aptasensor employs both types of electrodes, namely commercialized screen-printed electrodes (C-SPEs) and self-fabricated screen-printed electrodes (SF-SPEs), were efficiently diagnose dengue virus antigen (DENV-Ag) and shows a lower limit of detection (LOD) i.e., 0.1 μg/ml. Both the electrode types were coated with chemically synthesized ZnO-Nanomaterial, which aids in electron transport, and to make it more selective highly specific DNA-aptamer was used against the DENV antigen. SEM and Uv–Vis spectra approaches were used to characterize the synthesized nanomaterial. To confirm the DENV-antigen detection results, electrochemical analysis was performed and the sensor cross-reactivity was also checked by a close member of the dengue virus i.e., chikungunya virus (CHIKV). The developed platform based on SF-SPEs & C-SPEs performed well in human serum. This investigation found that the SF-SPEs system had advanced sensitivity and responded very well to the C-SPEs. Consequently, the SF-SPEs system has emerged as a feasible choice for low-cost and highly sensitive DENV-detection and is also applicable for other analytes diagnostics.http://www.sciencedirect.com/science/article/pii/S266635112400007XCommercialized electrodePaper electrodeDENV- diagnosisComparative analysis |
| spellingShingle | Mohd Rahil Hasan Pradakshina Sharma Saumitra Singh Sushil Kumar Shivani Kirti Rani Jagriti Narang Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study Sensors International Commercialized electrode Paper electrode DENV- diagnosis Comparative analysis |
| title | Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study |
| title_full | Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study |
| title_fullStr | Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study |
| title_full_unstemmed | Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study |
| title_short | Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study |
| title_sort | different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum a comparative study |
| topic | Commercialized electrode Paper electrode DENV- diagnosis Comparative analysis |
| url | http://www.sciencedirect.com/science/article/pii/S266635112400007X |
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