Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair
Objective. To assess the clinical value and safety of the application of allogeneic equine oral mucosa mesenchymal stromal cells (OM-MSCs) to wounds. Animals. 8 healthy adult horses without front limb skin lesions or musculoskeletal disease. Procedures. Stem cells were isolated from the oral mucosa...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2021/5024905 |
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| author | Paola Di Francesco Pauline Cajon Christophe Desterke Marie-France Perron Lepage Jean-Jacques Lataillade Tewfik Kadri Olivier M. Lepage |
| author_facet | Paola Di Francesco Pauline Cajon Christophe Desterke Marie-France Perron Lepage Jean-Jacques Lataillade Tewfik Kadri Olivier M. Lepage |
| author_sort | Paola Di Francesco |
| collection | DOAJ |
| description | Objective. To assess the clinical value and safety of the application of allogeneic equine oral mucosa mesenchymal stromal cells (OM-MSCs) to wounds. Animals. 8 healthy adult horses without front limb skin lesions or musculoskeletal disease. Procedures. Stem cells were isolated from the oral mucosa of a donor horse. Horses were subjected to the creation of eight full-thickness cutaneous wounds, two on each distal forelimb (FL) and two on both sides of the thorax (TH). Each wound was subjected to one out of four treatments: no medication (T1), hyaluronic acid- (HA-) gel containing OM-MSC (T2), HA-gel containing OM-MSC secretome (T3), and HA-gel alone (T4). Gross macroscopic evaluation and laser digital photographic documentation were regularly performed to allow wound assessment including wound surface area. Full-thickness skin punch biopsy was performed at each site before wound induction (D0, normal skin) and after complete wound healing (D62, repaired skin). Results. All wounds healed without adverse effect at D62. Distal limb wounds are slower to heal than body wounds. OM-MSC and its secretome have a positive impact on TH wound contraction. OM-MSC has a positive impact on the contraction and epithelialization of FL wounds. No significant difference between wound sites before and after treatment was noted at histological examination. Conclusion and Clinical Relevance. Using horse cells harvested from oral mucosa is a feasible technique to produce OM-MSC or its secretome. The gel produced by the combination of these biologic components with HA shows a positive impact when applied during the early stage of wound healing. |
| format | Article |
| id | doaj-art-820b16ade3294fc69432e683a2d99f20 |
| institution | OA Journals |
| issn | 2042-0048 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-820b16ade3294fc69432e683a2d99f202025-08-20T02:09:05ZengWileyVeterinary Medicine International2042-00482021-01-01202110.1155/2021/5024905Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound RepairPaola Di Francesco0Pauline Cajon1Christophe Desterke2Marie-France Perron Lepage3Jean-Jacques Lataillade4Tewfik Kadri5Olivier M. Lepage6Unité ICE-Groupe de Recherche en Médecine et Rééducation des Equidés de Sport (GREMERES)Stem Cell Vet Therapeutics SASINSERM UMR935Vet Tox Path ConsultingInstitut de Recherche Biomédical des ArméesStem Cell Vet Therapeutics SASUnité ICE-Groupe de Recherche en Médecine et Rééducation des Equidés de Sport (GREMERES)Objective. To assess the clinical value and safety of the application of allogeneic equine oral mucosa mesenchymal stromal cells (OM-MSCs) to wounds. Animals. 8 healthy adult horses without front limb skin lesions or musculoskeletal disease. Procedures. Stem cells were isolated from the oral mucosa of a donor horse. Horses were subjected to the creation of eight full-thickness cutaneous wounds, two on each distal forelimb (FL) and two on both sides of the thorax (TH). Each wound was subjected to one out of four treatments: no medication (T1), hyaluronic acid- (HA-) gel containing OM-MSC (T2), HA-gel containing OM-MSC secretome (T3), and HA-gel alone (T4). Gross macroscopic evaluation and laser digital photographic documentation were regularly performed to allow wound assessment including wound surface area. Full-thickness skin punch biopsy was performed at each site before wound induction (D0, normal skin) and after complete wound healing (D62, repaired skin). Results. All wounds healed without adverse effect at D62. Distal limb wounds are slower to heal than body wounds. OM-MSC and its secretome have a positive impact on TH wound contraction. OM-MSC has a positive impact on the contraction and epithelialization of FL wounds. No significant difference between wound sites before and after treatment was noted at histological examination. Conclusion and Clinical Relevance. Using horse cells harvested from oral mucosa is a feasible technique to produce OM-MSC or its secretome. The gel produced by the combination of these biologic components with HA shows a positive impact when applied during the early stage of wound healing.http://dx.doi.org/10.1155/2021/5024905 |
| spellingShingle | Paola Di Francesco Pauline Cajon Christophe Desterke Marie-France Perron Lepage Jean-Jacques Lataillade Tewfik Kadri Olivier M. Lepage Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair Veterinary Medicine International |
| title | Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair |
| title_full | Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair |
| title_fullStr | Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair |
| title_full_unstemmed | Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair |
| title_short | Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair |
| title_sort | effect of allogeneic oral mucosa mesenchymal stromal cells on equine wound repair |
| url | http://dx.doi.org/10.1155/2021/5024905 |
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