Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining
Experimental studies, though often very costly, lead to the discovery of known antimicrobial products. Yet, pathogenic microorganisms are proving increasingly resistant to pre-existing antimicrobial molecules, and this is a cause for worldwide concern. Therefore, it is necessary to search for new mo...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1515483/full |
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| author | Blaise Waongo Libère Ndayishimiye François Tapsoba Wend-Soo Abel Zongo Jinquan Li Aly Savadogo |
| author_facet | Blaise Waongo Libère Ndayishimiye François Tapsoba Wend-Soo Abel Zongo Jinquan Li Aly Savadogo |
| author_sort | Blaise Waongo |
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| description | Experimental studies, though often very costly, lead to the discovery of known antimicrobial products. Yet, pathogenic microorganisms are proving increasingly resistant to pre-existing antimicrobial molecules, and this is a cause for worldwide concern. Therefore, it is necessary to search for new molecules that could serve as alternatives in the food, medical and agricultural sectors. Thus, 123 complete genomes of Bacillus strains isolated from soil and fermented foods were analyzed and annotated using bioinformatics prediction and characterization tools. The view was to discover new gene clusters for the biosynthesis of non-ribosomal peptides (lipopeptides, siderophores, antibiotics). This study revealed that 83% of the genomes analyzed possess biosynthetic gene clusters for the production of siderophore bacillibactin, 61% for surfactins, 37% for fengycins, 23% for iturins, 15% for kurstakins and 3% for bacitracin. Besides, seven new biosynthetic gene clusters coding Non Ribosomal Peptide Synthetases (NRPS) have been identified in B. velezensis ATR2, B. velezensis DSYZ, B. velezensis CGMCC11640, B. amyloliquefaciens HM618, B. amyloliquefaciens WF02, B. cereus CMCC P0011, B. cereus CMCC P0021, B. subtilis SJ-10 and B. anthracis CMF9. The results of this study revealed a significant potential of the genus Bacillus to produce new non-ribosomally synthesized peptides. Now, these predicted new antimicrobial molecules can be easily studied experimentally as many new gene clusters have been identified. |
| format | Article |
| id | doaj-art-81fe86f81a9b4d329ee16b5ac613bd0d |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-81fe86f81a9b4d329ee16b5ac613bd0d2025-08-20T03:45:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.15154831515483Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome miningBlaise Waongo0Libère Ndayishimiye1François Tapsoba2Wend-Soo Abel Zongo3Jinquan Li4Aly Savadogo5Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou, Burkina FasoNational Key Laboratory of Agricultural Microbiology, Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, ChinaLaboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou, Burkina FasoCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou, ChinaNational Key Laboratory of Agricultural Microbiology, Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, ChinaLaboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou, Burkina FasoExperimental studies, though often very costly, lead to the discovery of known antimicrobial products. Yet, pathogenic microorganisms are proving increasingly resistant to pre-existing antimicrobial molecules, and this is a cause for worldwide concern. Therefore, it is necessary to search for new molecules that could serve as alternatives in the food, medical and agricultural sectors. Thus, 123 complete genomes of Bacillus strains isolated from soil and fermented foods were analyzed and annotated using bioinformatics prediction and characterization tools. The view was to discover new gene clusters for the biosynthesis of non-ribosomal peptides (lipopeptides, siderophores, antibiotics). This study revealed that 83% of the genomes analyzed possess biosynthetic gene clusters for the production of siderophore bacillibactin, 61% for surfactins, 37% for fengycins, 23% for iturins, 15% for kurstakins and 3% for bacitracin. Besides, seven new biosynthetic gene clusters coding Non Ribosomal Peptide Synthetases (NRPS) have been identified in B. velezensis ATR2, B. velezensis DSYZ, B. velezensis CGMCC11640, B. amyloliquefaciens HM618, B. amyloliquefaciens WF02, B. cereus CMCC P0011, B. cereus CMCC P0021, B. subtilis SJ-10 and B. anthracis CMF9. The results of this study revealed a significant potential of the genus Bacillus to produce new non-ribosomally synthesized peptides. Now, these predicted new antimicrobial molecules can be easily studied experimentally as many new gene clusters have been identified.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1515483/fullBacillusnonribosomal peptidelipopeptidebiosynthetic gene clustersgenome mining |
| spellingShingle | Blaise Waongo Libère Ndayishimiye François Tapsoba Wend-Soo Abel Zongo Jinquan Li Aly Savadogo Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining Frontiers in Microbiology Bacillus nonribosomal peptide lipopeptide biosynthetic gene clusters genome mining |
| title | Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining |
| title_full | Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining |
| title_fullStr | Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining |
| title_full_unstemmed | Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining |
| title_short | Prospection for potential new non-ribosomal peptide gene clusters in Bacillus genus isolated from fermented foods and soil through genome mining |
| title_sort | prospection for potential new non ribosomal peptide gene clusters in bacillus genus isolated from fermented foods and soil through genome mining |
| topic | Bacillus nonribosomal peptide lipopeptide biosynthetic gene clusters genome mining |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1515483/full |
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