ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY
Objective: Tribbles protein (TRIB) family was first identified in Drosophila, plays role in coordinating cell division and morphogenesis. TRIB3 is one of the members of this family, its protein expression levels and patient prognosis has been examined in various studies. While TRIB3 expression was c...
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Istanbul University Press
2022-08-01
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| author | Burçin Baran Gamze Sanlav Deniz Kızmazoğlu Selen Kum Özşengezer Safiye Aktaş Zekiye Altun Nur Olgun |
| author_facet | Burçin Baran Gamze Sanlav Deniz Kızmazoğlu Selen Kum Özşengezer Safiye Aktaş Zekiye Altun Nur Olgun |
| author_sort | Burçin Baran |
| collection | DOAJ |
| description | Objective: Tribbles protein (TRIB) family was first identified in Drosophila, plays role in coordinating cell division and morphogenesis. TRIB3 is one of the members of this family, its protein expression levels and patient prognosis has been examined in various studies. While TRIB3 expression was correlated with poor prognosis in hepatocellular cancer patients, its expression levels were associated with increased disease-free survival and a better response to therapy in luminal breast cancer patients. Neuroblastoma (NB) is the most common solid tumor in children and accounts for ~15% of childhood cancer‐related mortality. In the present study, we aimed the investigate the TRIB3 expression levels among NB patients and to determine if TRIB3 expression levels could be prognostic biomarker for NB. Materials and Methods: 40 children with different stages of NB were studied. The patients were staged according to criteria defined by Turkish Pediatric Oncology Group and International Neuroblastoma Staging System. Protein expression levels of TRIB3 was determined with immunohistochemical staining on paraffin sections of NB patient tissue samples. All sections were independently and blindly studied for protein expression under microscopic examination. P values <0.05 was accepted statistically significant. Results: TRIB3 expression was found to be upregulated in low grade samples and ganglioneuroblastoma samples and downregulated in neuroblastoma cells in the tissue sections of high-grade NB samples. Conclusion: While study is still ongoing, preliminary results showed that TRIB3 could be a candidate prognostic marker for NB patients. |
| format | Article |
| id | doaj-art-81fdf0f4329a485ea235459ea71c5c74 |
| institution | OA Journals |
| issn | 2651-4060 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Sabiad |
| spelling | doaj-art-81fdf0f4329a485ea235459ea71c5c742025-08-20T02:14:06ZengIstanbul University PressSabiad2651-40602022-08-0151565610.26650/JARHS2021-1137378123456ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDYBurçin Baran0https://orcid.org/0000-0002-0917-5443Gamze Sanlav1Deniz Kızmazoğlu2Selen Kum Özşengezer3Safiye Aktaş4Zekiye Altun5Nur Olgun6Dokuz Eylül Üniversitesi, Izmir, TurkiyeDokuz Eylül Üniversitesi, Izmir, TurkiyeDokuz Eylül Üniversitesi, Izmir, TurkiyeDokuz Eylül Üniversitesi, Izmir, TurkiyeDokuz Eylül Üniversitesi, Izmir, TurkiyeDokuz Eylül Üniversitesi, Izmir, TurkiyeDokuz Eylül Üniversitesi, Izmir, TurkiyeObjective: Tribbles protein (TRIB) family was first identified in Drosophila, plays role in coordinating cell division and morphogenesis. TRIB3 is one of the members of this family, its protein expression levels and patient prognosis has been examined in various studies. While TRIB3 expression was correlated with poor prognosis in hepatocellular cancer patients, its expression levels were associated with increased disease-free survival and a better response to therapy in luminal breast cancer patients. Neuroblastoma (NB) is the most common solid tumor in children and accounts for ~15% of childhood cancer‐related mortality. In the present study, we aimed the investigate the TRIB3 expression levels among NB patients and to determine if TRIB3 expression levels could be prognostic biomarker for NB. Materials and Methods: 40 children with different stages of NB were studied. The patients were staged according to criteria defined by Turkish Pediatric Oncology Group and International Neuroblastoma Staging System. Protein expression levels of TRIB3 was determined with immunohistochemical staining on paraffin sections of NB patient tissue samples. All sections were independently and blindly studied for protein expression under microscopic examination. P values <0.05 was accepted statistically significant. Results: TRIB3 expression was found to be upregulated in low grade samples and ganglioneuroblastoma samples and downregulated in neuroblastoma cells in the tissue sections of high-grade NB samples. Conclusion: While study is still ongoing, preliminary results showed that TRIB3 could be a candidate prognostic marker for NB patients.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/963CD03A504C4419B467E02DE4CAA534neuroblastomacell signalingbiomarkerprognostic marker |
| spellingShingle | Burçin Baran Gamze Sanlav Deniz Kızmazoğlu Selen Kum Özşengezer Safiye Aktaş Zekiye Altun Nur Olgun ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY Sabiad neuroblastoma cell signaling biomarker prognostic marker |
| title | ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY |
| title_full | ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY |
| title_fullStr | ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY |
| title_full_unstemmed | ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY |
| title_short | ANALYSIS OF TRIBBLES HOMOLOG 3 (TRIB3) EXPRESSION LEVELS IN NEUROBLASTOMA: PRELIMINARY STUDY |
| title_sort | analysis of tribbles homolog 3 trib3 expression levels in neuroblastoma preliminary study |
| topic | neuroblastoma cell signaling biomarker prognostic marker |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/963CD03A504C4419B467E02DE4CAA534 |
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