Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)

Purpose — to study the effect of a mid-infrared laser with a wavelength of 3.0 μm on the eye tissue. Materials and methods. An experimental laser device “Laser scalpel in the middle infrared range 2–8 μm for precision surgery” was used. The range of the radiation spectrum generated by this laser coi...

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Main Authors: Yu. N. Yusef, D. V. Petrachkov, E. N. Korobov, I. A. Novikov, M. V. Kravchik
Format: Article
Language:Russian
Published: Ophthalmology Publishing Group 2023-10-01
Series:Oftalʹmologiâ
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Online Access:https://www.ophthalmojournal.com/opht/article/view/2181
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author Yu. N. Yusef
D. V. Petrachkov
E. N. Korobov
I. A. Novikov
M. V. Kravchik
author_facet Yu. N. Yusef
D. V. Petrachkov
E. N. Korobov
I. A. Novikov
M. V. Kravchik
author_sort Yu. N. Yusef
collection DOAJ
description Purpose — to study the effect of a mid-infrared laser with a wavelength of 3.0 μm on the eye tissue. Materials and methods. An experimental laser device “Laser scalpel in the middle infrared range 2–8 μm for precision surgery” was used. The range of the radiation spectrum generated by this laser coincides with the spectral absorption peaks of such biological tissue chromophores as water, proteins and cholesterol esters, which makes it possible to use this laser in precision surgery (ophthalmology, neurosurgery, vascular surgery). In this study, we chose a wavelength of 3 µm, which coincides with the peak of the spectral absorption of water. The retina, choroid, sclera of porcine and cadaveric eyes was used as biological tissue. The effect of laser radiation on tissue was carried out in air. The application of coagulates was performed in different modes: 1) single pulses, 2) continuous mode. The impact of laser radiation on eye tissues was assessed using a scanning electron microscope. Results. When exposed to a single laser pulse with an energy of even 1 mJ, a through defect of the retina was observed. When exposed to a single laser pulse on the choroid with an energy of 1 to 3 mJ, the depth of the crater was 110–130 µm, the width of the crater was 97–122 µm, and the zone of collateral damage was 22–28 µm. When exposed to a single laser pulse on the sclera with an energy of 1 to 3 mJ, the depth of the crater was 170–201 µm, the width of the crater was 260–303 µm, and the zone of collateral damage was 57–72 µm. With continuous exposure to laser radiation on the choroid and sclera, the depth, width of the coagulate, the zone of collateral damage with similar laser parameters were greater than with a single pulse. Conclusion. Using this laser at a wavelength of 3 μm, it is possible to dissect eye tissues, which can be useful in vitreoretinal surgery for retinotomy, cutting out the choroid-pigment epithelium complex, and in antiglaucoma surgery for non-penetrating deep sclerectomy. However, further research is needed to optimize such laser parameters as the number of pulses in a packet, pulse duration, and pulse energy.
format Article
id doaj-art-81f2f7214095498c8e162a3819d4bbd2
institution Kabale University
issn 1816-5095
2500-0845
language Russian
publishDate 2023-10-01
publisher Ophthalmology Publishing Group
record_format Article
series Oftalʹmologiâ
spelling doaj-art-81f2f7214095498c8e162a3819d4bbd22025-08-20T03:38:18ZrusOphthalmology Publishing GroupOftalʹmologiâ1816-50952500-08452023-10-0120350851410.18008/1816-5095-2023-3-508-5141066Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)Yu. N. Yusef0D. V. Petrachkov1E. N. Korobov2I. A. Novikov3M. V. Kravchik4M.M. Krasnov Research Institute of Eye DiseaseM.M. Krasnov Research Institute of Eye DiseaseM.M. Krasnov Research Institute of Eye DiseaseM.M. Krasnov Research Institute of Eye DiseaseM.M. Krasnov Research Institute of Eye DiseasePurpose — to study the effect of a mid-infrared laser with a wavelength of 3.0 μm on the eye tissue. Materials and methods. An experimental laser device “Laser scalpel in the middle infrared range 2–8 μm for precision surgery” was used. The range of the radiation spectrum generated by this laser coincides with the spectral absorption peaks of such biological tissue chromophores as water, proteins and cholesterol esters, which makes it possible to use this laser in precision surgery (ophthalmology, neurosurgery, vascular surgery). In this study, we chose a wavelength of 3 µm, which coincides with the peak of the spectral absorption of water. The retina, choroid, sclera of porcine and cadaveric eyes was used as biological tissue. The effect of laser radiation on tissue was carried out in air. The application of coagulates was performed in different modes: 1) single pulses, 2) continuous mode. The impact of laser radiation on eye tissues was assessed using a scanning electron microscope. Results. When exposed to a single laser pulse with an energy of even 1 mJ, a through defect of the retina was observed. When exposed to a single laser pulse on the choroid with an energy of 1 to 3 mJ, the depth of the crater was 110–130 µm, the width of the crater was 97–122 µm, and the zone of collateral damage was 22–28 µm. When exposed to a single laser pulse on the sclera with an energy of 1 to 3 mJ, the depth of the crater was 170–201 µm, the width of the crater was 260–303 µm, and the zone of collateral damage was 57–72 µm. With continuous exposure to laser radiation on the choroid and sclera, the depth, width of the coagulate, the zone of collateral damage with similar laser parameters were greater than with a single pulse. Conclusion. Using this laser at a wavelength of 3 μm, it is possible to dissect eye tissues, which can be useful in vitreoretinal surgery for retinotomy, cutting out the choroid-pigment epithelium complex, and in antiglaucoma surgery for non-penetrating deep sclerectomy. However, further research is needed to optimize such laser parameters as the number of pulses in a packet, pulse duration, and pulse energy.https://www.ophthalmojournal.com/opht/article/view/2181vitreoretinal surgeryvitrectomyphotovitrectomylaser vitrectomynd:yag laserer:yag laserсо2 lasermidinfrared spectrumlaser ablationprecision surgery
spellingShingle Yu. N. Yusef
D. V. Petrachkov
E. N. Korobov
I. A. Novikov
M. V. Kravchik
Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)
Oftalʹmologiâ
vitreoretinal surgery
vitrectomy
photovitrectomy
laser vitrectomy
nd:yag laser
er:yag laser
со2 laser
midinfrared spectrum
laser ablation
precision surgery
title Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)
title_full Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)
title_fullStr Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)
title_full_unstemmed Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)
title_short Evaluation of the Impact of Mid-Infrared Laser Radiation on Eye Tissues (Experimental Study)
title_sort evaluation of the impact of mid infrared laser radiation on eye tissues experimental study
topic vitreoretinal surgery
vitrectomy
photovitrectomy
laser vitrectomy
nd:yag laser
er:yag laser
со2 laser
midinfrared spectrum
laser ablation
precision surgery
url https://www.ophthalmojournal.com/opht/article/view/2181
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AT dvpetrachkov evaluationoftheimpactofmidinfraredlaserradiationoneyetissuesexperimentalstudy
AT enkorobov evaluationoftheimpactofmidinfraredlaserradiationoneyetissuesexperimentalstudy
AT ianovikov evaluationoftheimpactofmidinfraredlaserradiationoneyetissuesexperimentalstudy
AT mvkravchik evaluationoftheimpactofmidinfraredlaserradiationoneyetissuesexperimentalstudy