Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding

<b>Background:</b> The purpose of this work was to demonstrate the ozone efficacy for disinfection of the PHARMODUCT<sup>®</sup> automatic dispensing system for antineoplastic preparation, as a guarantee of a higher grade of cleanliness. While the use of ozone gas disinfectio...

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Main Authors: Vito Lovino, Antonio Riglietti, Anna Tolomeo, Giuseppe Capasso, Miriana Di Vittorio, Stefano Brattoli, Giuseppe Tesse, Vincenzo Dimiccoli, Marco Spartà, Luana Perioli
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/18/2/140
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author Vito Lovino
Antonio Riglietti
Anna Tolomeo
Giuseppe Capasso
Miriana Di Vittorio
Stefano Brattoli
Giuseppe Tesse
Vincenzo Dimiccoli
Marco Spartà
Luana Perioli
author_facet Vito Lovino
Antonio Riglietti
Anna Tolomeo
Giuseppe Capasso
Miriana Di Vittorio
Stefano Brattoli
Giuseppe Tesse
Vincenzo Dimiccoli
Marco Spartà
Luana Perioli
author_sort Vito Lovino
collection DOAJ
description <b>Background:</b> The purpose of this work was to demonstrate the ozone efficacy for disinfection of the PHARMODUCT<sup>®</sup> automatic dispensing system for antineoplastic preparation, as a guarantee of a higher grade of cleanliness. While the use of ozone gas disinfection is almost consolidated in food and water treatment, there is a lack of scientific data in the pharmaceutical field. The scope of this study was to demonstrate the ozone efficacy for disinfection of the PHARMODUCT<sup>®</sup> automatic dispensing system, before starting the antineoplastic preparation, in order to ensure a high degree of cleanliness and, at the same time, to define a biodecontamination procedure that could also be translatable to other automated compounding systems on the market. <b>Methods</b>: Ozone efficacy was determined by calculating the difference (pre-exposure–post-exposure) in CFU counts on the plate. A group of four different ATCC-selected microbial strains were tested using two distinct cycles. The first one was evaluated with an ozone gas concentration of 40 ppm for 40 min; the second cycle increased the concentration to 60 ppm for the same duration. <b>Results</b>: Results showed that exposure to 40 ppm ozone gas led to a 4-log reduction of all tested ATCC strains. In contrast, exposure to 60 ppm ensured a 6-log reduction. <b>Conclusions:</b> The ozone disinfection process, applied to the PHARMODUCT<sup>®</sup> system, provides a superior grade of cleanliness compared to the manual disinfection procedure, thus offering insight beyond the current anti-inflammatory and analgesic application of ozone therapy in the medical field.
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spelling doaj-art-81f1dbb9aca145ad802e2891d4c60dc02025-08-20T02:04:06ZengMDPI AGPharmaceuticals1424-82472025-01-0118214010.3390/ph18020140Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic CompoundingVito Lovino0Antonio Riglietti1Anna Tolomeo2Giuseppe Capasso3Miriana Di Vittorio4Stefano Brattoli5Giuseppe Tesse6Vincenzo Dimiccoli7Marco Spartà8Luana Perioli9Itel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyItel Telecomunicazioni srl—Radiopharmaceutical Division, Via Antonio Labriola SNC, 70037 Ruvo di Puglia, ItalyBioduct s.r.l., Via Di Collodi, 6/C, 50141 Firenze, ItalyDepartment of Pharmaceutical Sciences, University of Perugia, Via del Liceo 1, 06123 Perugia, Italy<b>Background:</b> The purpose of this work was to demonstrate the ozone efficacy for disinfection of the PHARMODUCT<sup>®</sup> automatic dispensing system for antineoplastic preparation, as a guarantee of a higher grade of cleanliness. While the use of ozone gas disinfection is almost consolidated in food and water treatment, there is a lack of scientific data in the pharmaceutical field. The scope of this study was to demonstrate the ozone efficacy for disinfection of the PHARMODUCT<sup>®</sup> automatic dispensing system, before starting the antineoplastic preparation, in order to ensure a high degree of cleanliness and, at the same time, to define a biodecontamination procedure that could also be translatable to other automated compounding systems on the market. <b>Methods</b>: Ozone efficacy was determined by calculating the difference (pre-exposure–post-exposure) in CFU counts on the plate. A group of four different ATCC-selected microbial strains were tested using two distinct cycles. The first one was evaluated with an ozone gas concentration of 40 ppm for 40 min; the second cycle increased the concentration to 60 ppm for the same duration. <b>Results</b>: Results showed that exposure to 40 ppm ozone gas led to a 4-log reduction of all tested ATCC strains. In contrast, exposure to 60 ppm ensured a 6-log reduction. <b>Conclusions:</b> The ozone disinfection process, applied to the PHARMODUCT<sup>®</sup> system, provides a superior grade of cleanliness compared to the manual disinfection procedure, thus offering insight beyond the current anti-inflammatory and analgesic application of ozone therapy in the medical field.https://www.mdpi.com/1424-8247/18/2/140ATCCozoneautomatic compoundingrobot bio-decontaminationdisinfection
spellingShingle Vito Lovino
Antonio Riglietti
Anna Tolomeo
Giuseppe Capasso
Miriana Di Vittorio
Stefano Brattoli
Giuseppe Tesse
Vincenzo Dimiccoli
Marco Spartà
Luana Perioli
Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding
Pharmaceuticals
ATCC
ozone
automatic compounding
robot bio-decontamination
disinfection
title Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding
title_full Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding
title_fullStr Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding
title_full_unstemmed Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding
title_short Use of Ozone for Disinfection of PHARMODUCT<sup>®</sup> Automatic System for Antineoplastic Compounding
title_sort use of ozone for disinfection of pharmoduct sup r sup automatic system for antineoplastic compounding
topic ATCC
ozone
automatic compounding
robot bio-decontamination
disinfection
url https://www.mdpi.com/1424-8247/18/2/140
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