Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules
Fluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhi...
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| Format: | Article |
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/22/5237 |
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| author | Raquel Eustáquio João P. Prates Ramalho Sílvia Arantes António Candeias Ana Teresa Caldeira António Pereira |
| author_facet | Raquel Eustáquio João P. Prates Ramalho Sílvia Arantes António Candeias Ana Teresa Caldeira António Pereira |
| author_sort | Raquel Eustáquio |
| collection | DOAJ |
| description | Fluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhibiting small Stokes shifts. This limitation underscores the potential of 4-diethylaminobenzaldehyde derivatives as a cost-effective alternative for developing new, bright fluorophores with larger Stokes shifts. In this work, using 4-diethylaminobenzaldehyde as starting material, we developed a simple, cost-effective, and efficient synthetic strategy to produce new affordable small molecules as effective fluorescent labels for biomolecules. Density functional theory and time-dependent density functional theory calculations were also conducted to gain insights into the observed photophysical properties. |
| format | Article |
| id | doaj-art-81bd0ffcbbc4405aa315cc7c6aa0123b |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-81bd0ffcbbc4405aa315cc7c6aa0123b2025-08-20T01:49:12ZengMDPI AGMolecules1420-30492024-11-012922523710.3390/molecules29225237Affordable Small Molecules as Promising Fluorescent Labels for BiomoleculesRaquel Eustáquio0João P. Prates Ramalho1Sílvia Arantes2António Candeias3Ana Teresa Caldeira4António Pereira5HERCULES Laboratory, IN2PAST—Associate Laboratory for Research and Innovation in Heritage, Arts, Sustainability and Territory, University of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, IN2PAST—Associate Laboratory for Research and Innovation in Heritage, Arts, Sustainability and Territory, University of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, IN2PAST—Associate Laboratory for Research and Innovation in Heritage, Arts, Sustainability and Territory, University of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, IN2PAST—Associate Laboratory for Research and Innovation in Heritage, Arts, Sustainability and Territory, University of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, IN2PAST—Associate Laboratory for Research and Innovation in Heritage, Arts, Sustainability and Territory, University of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalHERCULES Laboratory, IN2PAST—Associate Laboratory for Research and Innovation in Heritage, Arts, Sustainability and Territory, University of Évora, Largo Marquês de Marialva 8, 7000-809 Évora, PortugalFluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhibiting small Stokes shifts. This limitation underscores the potential of 4-diethylaminobenzaldehyde derivatives as a cost-effective alternative for developing new, bright fluorophores with larger Stokes shifts. In this work, using 4-diethylaminobenzaldehyde as starting material, we developed a simple, cost-effective, and efficient synthetic strategy to produce new affordable small molecules as effective fluorescent labels for biomolecules. Density functional theory and time-dependent density functional theory calculations were also conducted to gain insights into the observed photophysical properties.https://www.mdpi.com/1420-3049/29/22/5237fluorescent labels4-diethylaminobenzaldehyde derivativesbiomoleculesRNA-FISH probes |
| spellingShingle | Raquel Eustáquio João P. Prates Ramalho Sílvia Arantes António Candeias Ana Teresa Caldeira António Pereira Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules Molecules fluorescent labels 4-diethylaminobenzaldehyde derivatives biomolecules RNA-FISH probes |
| title | Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules |
| title_full | Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules |
| title_fullStr | Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules |
| title_full_unstemmed | Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules |
| title_short | Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules |
| title_sort | affordable small molecules as promising fluorescent labels for biomolecules |
| topic | fluorescent labels 4-diethylaminobenzaldehyde derivatives biomolecules RNA-FISH probes |
| url | https://www.mdpi.com/1420-3049/29/22/5237 |
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