Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers
Rice is the staple food for more than one half of the world’s population. The major reason for dismal state of rice production and productivity worldwide is due to biotic and abiotic stresses. Among insect pests, rice leaf folder (Cnapholocrocis medinais) earlier considered as a minor pest has gain...
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Puspa Publishing House
2016-11-01
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| Series: | International Journal of Economic Plants |
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| Online Access: | https://ojs.pphouse.org/index.php/IJEP/article/view/4476 |
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| author | P. Venkata Ramana Rao S. Divyasri K. Vasanta Bhanu P. Naga Kumari J. Karteek M. Girija Rani B.N.V.S.R. Ravi Kumar N. Chamundeswari K. Mohanvishnuvardhan V. Roja P.V. Satyanarayana A. Vishnuvardhan Reddy |
| author_facet | P. Venkata Ramana Rao S. Divyasri K. Vasanta Bhanu P. Naga Kumari J. Karteek M. Girija Rani B.N.V.S.R. Ravi Kumar N. Chamundeswari K. Mohanvishnuvardhan V. Roja P.V. Satyanarayana A. Vishnuvardhan Reddy |
| author_sort | P. Venkata Ramana Rao |
| collection | DOAJ |
| description |
Rice is the staple food for more than one half of the world’s population. The major reason for dismal state of rice production and productivity worldwide is due to biotic and abiotic stresses. Among insect pests, rice leaf folder (Cnapholocrocis medinais) earlier considered as a minor pest has gained the status of major pest with the widespread cultivation of HYV and the accompanying changes in cultural practices. Lack of reliable resistant donors is forcing the farmers to depend on insecticides to combat the pest. So, there is an urgent need to identify resistant donors and develop resistant varieties against leaf folder. The present study was carried out to study the extent of variability and molecular genetic diversity for leaf folder resistance in 30 genotypes including susceptible check TN1 under natural conditions. Based on the mean damage score (25, 50 and 75 DAT), 21 genotypes were resistant, 8 were moderately resistant and TN1 was susceptible. Correlation studies among different traits revealed that the trait damage score recorded significant positive correlation with leaf width (r= 0.180**) and SPAD chlorophyll meter reading (r= 0.136**) and non significant negative correlation with leaf length. To study the molecular diversity, 60 microsatellite markers were utilized. UPGMA analysis using NTSYS software grouped the 30 rice genotypes in to three clusters (Cluster I – 1genotype, Cluster II- 11 genotypes and Cluster III – 18 genotypes). UPGMA analysis using Darwin software grouped the 30 rice genotypes in to four clusters (Cluster I – 11 genotypes, Cluster II- 2 genotypes, Cluster III- 7 genotypes and Cluster IV – 10 genotypes). Based on the results it can be concluded that large variability exists in genotypes for leaf folder resistance which can be utilized in breeding programmes to develop rice varieties with resistance to leaf folder coupled with good grain quality and higher yield.
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| format | Article |
| id | doaj-art-81b67dc938b7477ba2ca534ed4994d02 |
| institution | OA Journals |
| issn | 2349-4735 |
| language | English |
| publishDate | 2016-11-01 |
| publisher | Puspa Publishing House |
| record_format | Article |
| series | International Journal of Economic Plants |
| spelling | doaj-art-81b67dc938b7477ba2ca534ed4994d022025-08-20T01:56:34ZengPuspa Publishing HouseInternational Journal of Economic Plants2349-47352016-11-013Nov, 4Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite MarkersP. Venkata Ramana Rao0S. Divyasri1K. Vasanta Bhanu2P. Naga Kumari3J. Karteek4M. Girija Rani5B.N.V.S.R. Ravi Kumar6N. Chamundeswari7K. Mohanvishnuvardhan8V. Roja9P.V. Satyanarayana10A. Vishnuvardhan Reddy11APRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaDept. of Biotechnology, Y.N. College, Narsapur - , West Godavari Dist., Andhra Pradesh (534 275), IndiaDAATTC, Machilipatnam-Krishna Dist., Andhra Pradesh (521 001), IndiaAPRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaAPRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaAPRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaAPRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaAPRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaRARS, Nandyal, Kurnool Dist., Andhra Pradesh (518 502), IndiaAgricultural Polytechnic, Anakapalle, Vishakpatnam Dist., Andhra Pradesh (531001), IndiaAPRRI& RARS, ANGRAU, Maruteru, West Godavari dist., Andhra Pradesh (534 122), IndiaIndian Institute of Oilseeds Research, Rajendranagar, Hyderabad, Telanagana (500 030), India Rice is the staple food for more than one half of the world’s population. The major reason for dismal state of rice production and productivity worldwide is due to biotic and abiotic stresses. Among insect pests, rice leaf folder (Cnapholocrocis medinais) earlier considered as a minor pest has gained the status of major pest with the widespread cultivation of HYV and the accompanying changes in cultural practices. Lack of reliable resistant donors is forcing the farmers to depend on insecticides to combat the pest. So, there is an urgent need to identify resistant donors and develop resistant varieties against leaf folder. The present study was carried out to study the extent of variability and molecular genetic diversity for leaf folder resistance in 30 genotypes including susceptible check TN1 under natural conditions. Based on the mean damage score (25, 50 and 75 DAT), 21 genotypes were resistant, 8 were moderately resistant and TN1 was susceptible. Correlation studies among different traits revealed that the trait damage score recorded significant positive correlation with leaf width (r= 0.180**) and SPAD chlorophyll meter reading (r= 0.136**) and non significant negative correlation with leaf length. To study the molecular diversity, 60 microsatellite markers were utilized. UPGMA analysis using NTSYS software grouped the 30 rice genotypes in to three clusters (Cluster I – 1genotype, Cluster II- 11 genotypes and Cluster III – 18 genotypes). UPGMA analysis using Darwin software grouped the 30 rice genotypes in to four clusters (Cluster I – 11 genotypes, Cluster II- 2 genotypes, Cluster III- 7 genotypes and Cluster IV – 10 genotypes). Based on the results it can be concluded that large variability exists in genotypes for leaf folder resistance which can be utilized in breeding programmes to develop rice varieties with resistance to leaf folder coupled with good grain quality and higher yield. https://ojs.pphouse.org/index.php/IJEP/article/view/4476Rice, leaf folder, microsatellite marker, molecular diversity |
| spellingShingle | P. Venkata Ramana Rao S. Divyasri K. Vasanta Bhanu P. Naga Kumari J. Karteek M. Girija Rani B.N.V.S.R. Ravi Kumar N. Chamundeswari K. Mohanvishnuvardhan V. Roja P.V. Satyanarayana A. Vishnuvardhan Reddy Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers International Journal of Economic Plants Rice, leaf folder, microsatellite marker, molecular diversity |
| title | Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers |
| title_full | Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers |
| title_fullStr | Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers |
| title_full_unstemmed | Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers |
| title_short | Molecular Characterization and Diversity Analysis for Leaf Folder Resistancein Rice Using Microsatellite Markers |
| title_sort | molecular characterization and diversity analysis for leaf folder resistancein rice using microsatellite markers |
| topic | Rice, leaf folder, microsatellite marker, molecular diversity |
| url | https://ojs.pphouse.org/index.php/IJEP/article/view/4476 |
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