Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2
IntroductionTwo significant barriers to skin wound care are severe inflammatory cascade reactions and microbial infections. The metabolites of probiotics contain various components, such as lactic acid and bacteriocins, which can synergistically maintain skin microecological balance and promote woun...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Materials |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmats.2025.1573222/full |
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| author | Yizhen Li Yizhen Li Xu Tang Xu Tang Yang Liu Yang Liu Xiaofeng Chen Xiaofeng Chen Ling Wang Ling Wang Yanjun Su Wei He Jingjing Li Qinmiao Huang Peng Wu Peng Wu |
| author_facet | Yizhen Li Yizhen Li Xu Tang Xu Tang Yang Liu Yang Liu Xiaofeng Chen Xiaofeng Chen Ling Wang Ling Wang Yanjun Su Wei He Jingjing Li Qinmiao Huang Peng Wu Peng Wu |
| author_sort | Yizhen Li |
| collection | DOAJ |
| description | IntroductionTwo significant barriers to skin wound care are severe inflammatory cascade reactions and microbial infections. The metabolites of probiotics contain various components, such as lactic acid and bacteriocins, which can synergistically maintain skin microecological balance and promote wound healing and tissue regeneration through mechanisms.MethodsThis study utilized lactic acid bacteria isolated from the whale intestine. Inspired by the concept of the whale’s skin self-repair ability and the influence of gut microbiota on the skin, this study ingeniously utilized the acidic properties of the lactic acid bacteria fermentation broth to dissolve chitosan (CS), forming a hydrogel while simultaneously incorporating the cell-free probiotic metabolites (CFPM) of Lactiplantibacillus plantarum HJ-S2 into a stable three-dimensional network structure. Various characterizations were tested, including water content, swelling ability, rheological properties and degradability.ResultsThis resulted in the creation of a medical hydrogel capable of promoting skin wound healing, named PM@CS hydrogel. PM@CS hydrogel exhibited excellent swelling ability in different liquid environment. FT-IR analysis showed that the hydrogel is successfully crosslinked, and thermalgravimetric analysis showed that PM@CS hydrogel was stably degraded under high temperature. PM@CS hydrogel and CFPM of HJ-S2 strongly inhibited the growth of E. coli and S. aureus. The organic acid content of CFPM was analyzed by HPLC, revealing the lactic acid and acetic acid were produced in large quantities as two main organic acid metabolites that may contribute to the antibacterial efficacy. Beyond the outstanding antibacterial ability, application of PM@CS hydrogel led to rapid healing of burn wounds, and diabetic wounds.DiscussionThe results indicated that the whale-derived probiotics have significant potential for application in treating burns and scalds, particularly in the treatment of diabetic wounds. |
| format | Article |
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| institution | Kabale University |
| issn | 2296-8016 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Materials |
| spelling | doaj-art-81b0b427471d4e5db13ef5bf5d8bfdd92025-08-20T03:51:59ZengFrontiers Media S.A.Frontiers in Materials2296-80162025-05-011210.3389/fmats.2025.15732221573222Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2Yizhen Li0Yizhen Li1Xu Tang2Xu Tang3Yang Liu4Yang Liu5Xiaofeng Chen6Xiaofeng Chen7Ling Wang8Ling Wang9Yanjun Su10Wei He11Jingjing Li12Qinmiao Huang13Peng Wu14Peng Wu15Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaFujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaFujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaFujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaFujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Xiamen, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaFujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Xiamen, ChinaStomatological Hospital of Xiamen Medical College, Xiamen, ChinaChina Tobacco Fujian Industrial CO., LTD., Xiamen, ChinaChina Tobacco Fujian Industrial CO., LTD., Xiamen, ChinaFujian Huijing Biological Technology CO., LTD., Zhangzhou, ChinaThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaFujian Provincial Key Laboratory of Island Conservation and Development (Island Research Center, MNR), Xiamen, ChinaIntroductionTwo significant barriers to skin wound care are severe inflammatory cascade reactions and microbial infections. The metabolites of probiotics contain various components, such as lactic acid and bacteriocins, which can synergistically maintain skin microecological balance and promote wound healing and tissue regeneration through mechanisms.MethodsThis study utilized lactic acid bacteria isolated from the whale intestine. Inspired by the concept of the whale’s skin self-repair ability and the influence of gut microbiota on the skin, this study ingeniously utilized the acidic properties of the lactic acid bacteria fermentation broth to dissolve chitosan (CS), forming a hydrogel while simultaneously incorporating the cell-free probiotic metabolites (CFPM) of Lactiplantibacillus plantarum HJ-S2 into a stable three-dimensional network structure. Various characterizations were tested, including water content, swelling ability, rheological properties and degradability.ResultsThis resulted in the creation of a medical hydrogel capable of promoting skin wound healing, named PM@CS hydrogel. PM@CS hydrogel exhibited excellent swelling ability in different liquid environment. FT-IR analysis showed that the hydrogel is successfully crosslinked, and thermalgravimetric analysis showed that PM@CS hydrogel was stably degraded under high temperature. PM@CS hydrogel and CFPM of HJ-S2 strongly inhibited the growth of E. coli and S. aureus. The organic acid content of CFPM was analyzed by HPLC, revealing the lactic acid and acetic acid were produced in large quantities as two main organic acid metabolites that may contribute to the antibacterial efficacy. Beyond the outstanding antibacterial ability, application of PM@CS hydrogel led to rapid healing of burn wounds, and diabetic wounds.DiscussionThe results indicated that the whale-derived probiotics have significant potential for application in treating burns and scalds, particularly in the treatment of diabetic wounds.https://www.frontiersin.org/articles/10.3389/fmats.2025.1573222/fullhydrogelLactiplantibacillus Plantarumprobiotic metabolitesburnsdiabetic |
| spellingShingle | Yizhen Li Yizhen Li Xu Tang Xu Tang Yang Liu Yang Liu Xiaofeng Chen Xiaofeng Chen Ling Wang Ling Wang Yanjun Su Wei He Jingjing Li Qinmiao Huang Peng Wu Peng Wu Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2 Frontiers in Materials hydrogel Lactiplantibacillus Plantarum probiotic metabolites burns diabetic |
| title | Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2 |
| title_full | Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2 |
| title_fullStr | Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2 |
| title_full_unstemmed | Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2 |
| title_short | Advanced wound healing with chitosan hydrogels incorporating metabolites from whale-derived Lactiplantibacillus plantarum HJ-S2 |
| title_sort | advanced wound healing with chitosan hydrogels incorporating metabolites from whale derived lactiplantibacillus plantarum hj s2 |
| topic | hydrogel Lactiplantibacillus Plantarum probiotic metabolites burns diabetic |
| url | https://www.frontiersin.org/articles/10.3389/fmats.2025.1573222/full |
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