Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways
The chalcone isomerase gene OsCHI, one of the key genes in the flavonoid biosynthesis pathway, plays an important role in rice (Oryza sativa) resistance to abiotic stresses. This study reveals how the chalcone isomerase gene family member OsCHI3 participates in rice responses to drought stress throu...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2025-04-01
|
| Series: | Crop Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514125000510 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850189167621832704 |
|---|---|
| author | Ting Liu Ling Liu Tianshun Zhou Yinke Chen Huang Zhou Jiahan Lyu Di Zhang Xiwen Shi Dingyang Yuan Nenghui Ye Meijuan Duan |
| author_facet | Ting Liu Ling Liu Tianshun Zhou Yinke Chen Huang Zhou Jiahan Lyu Di Zhang Xiwen Shi Dingyang Yuan Nenghui Ye Meijuan Duan |
| author_sort | Ting Liu |
| collection | DOAJ |
| description | The chalcone isomerase gene OsCHI, one of the key genes in the flavonoid biosynthesis pathway, plays an important role in rice (Oryza sativa) resistance to abiotic stresses. This study reveals how the chalcone isomerase gene family member OsCHI3 participates in rice responses to drought stress through the regulation of flavonoid biosynthesis. Overexpression of OsCHI3 increased the tolerance of rice to drought stress. In contrast, CRISPR/Cas9-mediated deletion of OsCHI3 reduced the drought tolerance of rice, an effect that is reversed by exogenous ABA treatment. Transcriptomic and physiological biochemical analyses indicated that flavonoids regulated by OsCHI3 not only scavenge reactive oxygen species (ROS) but also increase drought tolerance in rice by stimulating ABA biosynthesis through the regulation of OsNCED1 and OsABA8ox3 expression. These findings demonstrate that OsCHI3 increases drought stress tolerance in rice by activating the antioxidant defense system and the ABA metabolic pathway, providing new clues for drought-resistant rice breeding research. |
| format | Article |
| id | doaj-art-81a04a7b77684c8dbd1e97288ed6c0a7 |
| institution | OA Journals |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-81a04a7b77684c8dbd1e97288ed6c0a72025-08-20T02:15:41ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-04-0113237238410.1016/j.cj.2025.01.019Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathwaysTing Liu0Ling Liu1Tianshun Zhou2Yinke Chen3Huang Zhou4Jiahan Lyu5Di Zhang6Xiwen Shi7Dingyang Yuan8Nenghui Ye9Meijuan Duan10Hunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, Hunan, ChinaHunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaHunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, ChinaState Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, Hunan, China; Corresponding authors.Hunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, China; Corresponding authors.Hunan Provincial Key Laboratory of Rice Stress Biology, College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, China; Hunan Women’s University, Changsha 410004, Hunan, China; Corresponding authors.The chalcone isomerase gene OsCHI, one of the key genes in the flavonoid biosynthesis pathway, plays an important role in rice (Oryza sativa) resistance to abiotic stresses. This study reveals how the chalcone isomerase gene family member OsCHI3 participates in rice responses to drought stress through the regulation of flavonoid biosynthesis. Overexpression of OsCHI3 increased the tolerance of rice to drought stress. In contrast, CRISPR/Cas9-mediated deletion of OsCHI3 reduced the drought tolerance of rice, an effect that is reversed by exogenous ABA treatment. Transcriptomic and physiological biochemical analyses indicated that flavonoids regulated by OsCHI3 not only scavenge reactive oxygen species (ROS) but also increase drought tolerance in rice by stimulating ABA biosynthesis through the regulation of OsNCED1 and OsABA8ox3 expression. These findings demonstrate that OsCHI3 increases drought stress tolerance in rice by activating the antioxidant defense system and the ABA metabolic pathway, providing new clues for drought-resistant rice breeding research.http://www.sciencedirect.com/science/article/pii/S2214514125000510Chalcone isomerase geneFlavonoidsAbscisic acidDrought toleranceRice |
| spellingShingle | Ting Liu Ling Liu Tianshun Zhou Yinke Chen Huang Zhou Jiahan Lyu Di Zhang Xiwen Shi Dingyang Yuan Nenghui Ye Meijuan Duan Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways Crop Journal Chalcone isomerase gene Flavonoids Abscisic acid Drought tolerance Rice |
| title | Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways |
| title_full | Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways |
| title_fullStr | Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways |
| title_full_unstemmed | Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways |
| title_short | Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways |
| title_sort | chalcone isomerase gene oschi3 increases rice drought tolerance by scavenging ros via flavonoid and aba metabolic pathways |
| topic | Chalcone isomerase gene Flavonoids Abscisic acid Drought tolerance Rice |
| url | http://www.sciencedirect.com/science/article/pii/S2214514125000510 |
| work_keys_str_mv | AT tingliu chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT lingliu chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT tianshunzhou chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT yinkechen chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT huangzhou chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT jiahanlyu chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT dizhang chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT xiwenshi chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT dingyangyuan chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT nenghuiye chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways AT meijuanduan chalconeisomerasegeneoschi3increasesricedroughttolerancebyscavengingrosviaflavonoidandabametabolicpathways |