Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies

Peanut rust, caused by <i>Puccinia arachidis</i> Speg., is one of the most significant leaf diseases globally, and has a severe impact on peanut yield and quality. The development of disease-resistant varieties is recognized as an effective strategy to mitigate the damage caused by peanu...

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Main Authors: Xinlong Shi, Ziqi Sun, Feiyan Qi, Suoyi Han, Yixiong Zheng, Wenzhao Dong, Maoning Zhang, Xinyou Zhang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/8/1219
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author Xinlong Shi
Ziqi Sun
Feiyan Qi
Suoyi Han
Yixiong Zheng
Wenzhao Dong
Maoning Zhang
Xinyou Zhang
author_facet Xinlong Shi
Ziqi Sun
Feiyan Qi
Suoyi Han
Yixiong Zheng
Wenzhao Dong
Maoning Zhang
Xinyou Zhang
author_sort Xinlong Shi
collection DOAJ
description Peanut rust, caused by <i>Puccinia arachidis</i> Speg., is one of the most significant leaf diseases globally, and has a severe impact on peanut yield and quality. The development of disease-resistant varieties is recognized as an effective strategy to mitigate the damage caused by peanut rust. However, the research foundation for understanding peanut rust remains relatively limited. In this study, we identified significant single nucleotide polymorphisms (SNPs) associated with peanut rust resistance using a natural population consisting of 353 peanut germplasm accessions. These accessions were analyzed based on resequencing data and rust disease phenotypes across one laboratory test and three field trials. A total of 18 significant SNPs were identified on chromosomes A05 (5 SNPs), A08 (7 SNPs), and A12 (6 SNPs). Notably, three SNPs—Arahy.05_93085395, Arahy.05_93114354, and Arahy.12_4097252—were consistently detected across multiple environments. Within their confidence intervals, 48 genes were annotated, including 9 NLR domain-containing genes functionally related to plant disease resistance, which may serve as candidate genes for peanut rust resistance. This study provides insights into the regulatory mechanisms underlying peanut rust resistance.
format Article
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institution OA Journals
issn 2223-7747
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Plants
spelling doaj-art-8190e48457fa46c1911dcb6b306884f82025-08-20T02:28:19ZengMDPI AGPlants2223-77472025-04-01148121910.3390/plants14081219Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association StudiesXinlong Shi0Ziqi Sun1Feiyan Qi2Suoyi Han3Yixiong Zheng4Wenzhao Dong5Maoning Zhang6Xinyou Zhang7The Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaThe Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaThe Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaThe Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaCollege of Agriculture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaThe Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaThe Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaThe Shennong Laboratory/Institute of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaPeanut rust, caused by <i>Puccinia arachidis</i> Speg., is one of the most significant leaf diseases globally, and has a severe impact on peanut yield and quality. The development of disease-resistant varieties is recognized as an effective strategy to mitigate the damage caused by peanut rust. However, the research foundation for understanding peanut rust remains relatively limited. In this study, we identified significant single nucleotide polymorphisms (SNPs) associated with peanut rust resistance using a natural population consisting of 353 peanut germplasm accessions. These accessions were analyzed based on resequencing data and rust disease phenotypes across one laboratory test and three field trials. A total of 18 significant SNPs were identified on chromosomes A05 (5 SNPs), A08 (7 SNPs), and A12 (6 SNPs). Notably, three SNPs—Arahy.05_93085395, Arahy.05_93114354, and Arahy.12_4097252—were consistently detected across multiple environments. Within their confidence intervals, 48 genes were annotated, including 9 NLR domain-containing genes functionally related to plant disease resistance, which may serve as candidate genes for peanut rust resistance. This study provides insights into the regulatory mechanisms underlying peanut rust resistance.https://www.mdpi.com/2223-7747/14/8/1219peanutpeanut rust resistanceGWASSNPscandidate gene
spellingShingle Xinlong Shi
Ziqi Sun
Feiyan Qi
Suoyi Han
Yixiong Zheng
Wenzhao Dong
Maoning Zhang
Xinyou Zhang
Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies
Plants
peanut
peanut rust resistance
GWAS
SNPs
candidate gene
title Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies
title_full Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies
title_fullStr Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies
title_full_unstemmed Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies
title_short Identification of Genomic Regions Associated with Peanut Rust Resistance by Genome-Wide Association Studies
title_sort identification of genomic regions associated with peanut rust resistance by genome wide association studies
topic peanut
peanut rust resistance
GWAS
SNPs
candidate gene
url https://www.mdpi.com/2223-7747/14/8/1219
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AT yixiongzheng identificationofgenomicregionsassociatedwithpeanutrustresistancebygenomewideassociationstudies
AT wenzhaodong identificationofgenomicregionsassociatedwithpeanutrustresistancebygenomewideassociationstudies
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