Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures.
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery vectors in life sciences. However, Ad5 is dependent on expression of the coxsackievirus-adenovirus-receptor (CAR) on the surface of target cell for efficient transduction, which limits it's utility...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0054952&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849470300295528448 |
|---|---|
| author | Di Yu Chuan Jin Mohanraj Ramachandran Jing Xu Berith Nilsson Olle Korsgren Katarina Le Blanc Lene Uhrbom Karin Forsberg-Nilsson Bengt Westermark Rachel Adamson Norman Maitland Xiaolong Fan Magnus Essand |
| author_facet | Di Yu Chuan Jin Mohanraj Ramachandran Jing Xu Berith Nilsson Olle Korsgren Katarina Le Blanc Lene Uhrbom Karin Forsberg-Nilsson Bengt Westermark Rachel Adamson Norman Maitland Xiaolong Fan Magnus Essand |
| author_sort | Di Yu |
| collection | DOAJ |
| description | Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery vectors in life sciences. However, Ad5 is dependent on expression of the coxsackievirus-adenovirus-receptor (CAR) on the surface of target cell for efficient transduction, which limits it's utility for certain cell types. Herein we present a new vector, Ad5PTDf35, which is an Ad5 vector having serotype 35 fiber-specificity and Tat-PTD hexon-modification. This vector shows dramatically increased transduction capacity of primary human cell cultures including T cells, monocytes, macrophages, dendritic cells, pancreatic islets and exocrine cells, mesenchymal stem cells and tumor initiating cells. Biodistribution in mice following systemic administration (tail-vein injection) show significantly reduced uptake in the liver and spleen of Ad5PTDf35 compared to unmodified Ad5. Therefore, replication-competent viruses with these modifications may be further developed as oncolytic agents for cancer therapy. User-friendly backbone plasmids containing these modifications were developed for compatibility to the AdEasy-system to facilitate the development of surface-modified adenoviruses for gene delivery to difficult-to-transduce cells in basic, pre-clinical and clinical research. |
| format | Article |
| id | doaj-art-81845b54f6f045ed86a4443cbfdc2970 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-81845b54f6f045ed86a4443cbfdc29702025-08-20T03:25:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5495210.1371/journal.pone.0054952Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures.Di YuChuan JinMohanraj RamachandranJing XuBerith NilssonOlle KorsgrenKatarina Le BlancLene UhrbomKarin Forsberg-NilssonBengt WestermarkRachel AdamsonNorman MaitlandXiaolong FanMagnus EssandRecombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery vectors in life sciences. However, Ad5 is dependent on expression of the coxsackievirus-adenovirus-receptor (CAR) on the surface of target cell for efficient transduction, which limits it's utility for certain cell types. Herein we present a new vector, Ad5PTDf35, which is an Ad5 vector having serotype 35 fiber-specificity and Tat-PTD hexon-modification. This vector shows dramatically increased transduction capacity of primary human cell cultures including T cells, monocytes, macrophages, dendritic cells, pancreatic islets and exocrine cells, mesenchymal stem cells and tumor initiating cells. Biodistribution in mice following systemic administration (tail-vein injection) show significantly reduced uptake in the liver and spleen of Ad5PTDf35 compared to unmodified Ad5. Therefore, replication-competent viruses with these modifications may be further developed as oncolytic agents for cancer therapy. User-friendly backbone plasmids containing these modifications were developed for compatibility to the AdEasy-system to facilitate the development of surface-modified adenoviruses for gene delivery to difficult-to-transduce cells in basic, pre-clinical and clinical research.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0054952&type=printable |
| spellingShingle | Di Yu Chuan Jin Mohanraj Ramachandran Jing Xu Berith Nilsson Olle Korsgren Katarina Le Blanc Lene Uhrbom Karin Forsberg-Nilsson Bengt Westermark Rachel Adamson Norman Maitland Xiaolong Fan Magnus Essand Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures. PLoS ONE |
| title | Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures. |
| title_full | Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures. |
| title_fullStr | Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures. |
| title_full_unstemmed | Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures. |
| title_short | Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures. |
| title_sort | adenovirus serotype 5 vectors with tat ptd modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0054952&type=printable |
| work_keys_str_mv | AT diyu adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT chuanjin adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT mohanrajramachandran adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT jingxu adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT berithnilsson adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT ollekorsgren adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT katarinaleblanc adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT leneuhrbom adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT karinforsbergnilsson adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT bengtwestermark adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT racheladamson adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT normanmaitland adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT xiaolongfan adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures AT magnusessand adenovirusserotype5vectorswithtatptdmodifiedhexonandserotype35fibershowgreatlyenhancedtransductioncapacityofprimarycellcultures |