Screening, fermentation conditions and application potential of a bio-organic fertilizer strain against tomato gray mold
Tomato gray mold is a common disease which is caused by Botrytis cinerea and does harm to tomato seriously. Chemical control of tomato gray mold is easy to produce negative effects, such as drug resistance, pesticide residues and ecological imbalance. Biological control has advantages of high effici...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2014-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.09.182 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Tomato gray mold is a common disease which is caused by Botrytis cinerea and does harm to tomato seriously. Chemical control of tomato gray mold is easy to produce negative effects, such as drug resistance, pesticide residues and ecological imbalance. Biological control has advantages of high efficiency, no pollution, no drug resistance and combining prevention with treatment. Bio-organic fertilizer has both superiorities of bio-fertilizer and organic fertilizer, which can not only increase production, improve product quality, but also regulate and improve the composition of soil microorganisms, and then reduce the danger of crop diseases to some extent. Screening effective antagonistic strains of tomato gray mold and then preparing antagonistic agent and bio-organic fertilizer are important biotechnology ways to prevent and control tomato gray mold. The studies on solid fermentation of antagonistic strains are less involved at home and abroad.This study aimed at screening an effective antagonistic strain of tomato gray mold, exploring the solid fermentation conditions, preparing biological organic fertilizer and studying its bio-control effect on tomato gray mold.The strains of soil samples were isolated by the method of dilution coated tablet and streak plate, and then screened by tablet confrontation. Furthermore, they were identified by morphological and ITS sequence analysis. The influence factors on the conidial concentration of this strain were investigated by single factor and orthogonal experiments, including solid medium, external carbon, external nitrogen, external phosphate, the ratio of solid material to water, amount of bottled solid material, inoculums percentage, illumination, temperature and time of fermentation. The antagonistic strain was added to the rotten organic fertilizer to ferment, and then it was applied to pot experiment.After isolating, primary screening and rescreening, a strain of number Z47 was obtained eventually, which had the best inhibition effect and its inhibition rate reached 74.80% on B. cinerea. The strain was identified as Penicillium oxalicum. Fermentation condition optimization results for the P. oxalicum Z47 were as follows. The orthogonal optimization results for powdered rice hulls∶bran, maltose, ammonium sulfate, and dipotassium hydrogen phosphate in medium formula were 8∶2, 1.5%, 2% and 0.15%, respectively. The orthogonal optimization results for the ratio of solid material to water, amount of bottled solid material, inoculums percentage, illumination conditions and fermentation temperature in medium formula were 1∶0.6 (g/mL), 15 g, 12.5% (mL/g), dark totally and fermentation at a fluctuant temperature of 25—20—28℃ for 2 days, respectively.P. oxalicum Z47 had the highest spore number when cultured for 12 days under the optimal solid medium and culture conditions. The result of the pot experiment showed that the control effect of tomato gray mold by applying biological organic fertilizer which was rich in antagonistic strain Z47 was up to 73.67%, which was significantly higher than the treatment of antagonistic strain Z47 liquid.In sum, under the optimization of solid fermentation, the spore numbers of P. oxalicum Z47 were up to 5×10<sup>9</sup> CFU/g. The fermentation of biological organic fertilizer has good potential for prevention of tomato gray mold. |
|---|---|
| ISSN: | 1008-9209 2097-5155 |