Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast
Transient exposures to environmental stresses induce altered physiological states in exposed cells that persist after the stresses have been removed. These states, referred to as cellular memory, can even be passed on to daughter cells and may thus be thought of as embodying a form of epigenetic inh...
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Shared Science Publishers OG
2018-10-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/nutritional-and-meiotic-induction-of-transiently-heritable-stress-resistant-states-in-budding-yeast/ |
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| author | Heldder Gutierrez Bakhtiyar Taghizada Marc D. Meneghini |
| author_facet | Heldder Gutierrez Bakhtiyar Taghizada Marc D. Meneghini |
| author_sort | Heldder Gutierrez |
| collection | DOAJ |
| description | Transient exposures to environmental stresses induce altered physiological states in exposed cells that persist after the stresses have been removed. These states, referred to as cellular memory, can even be passed on to daughter cells and may thus be thought of as embodying a form of epigenetic inheritance. We find that meiotically produced spores in the budding yeast S. cerevisiae possess a state of heightened stress resistance that, following their germination, persists for numerous mitotic generations. As yeast meiotic development is essentially a starvation response that a/alpha diploid cells engage, we sought to model this phenomenon by subjecting haploid cells to starvation conditions. We find also that haploid cells exposed to glucose withdrawal acquire a state of elevated stress resistance that persists after the reintroduction of these cells to glucose-replete media. Following release from lengthy durations of glucose starvation, we confirm that this physiological state of enhanced stress resistance is propagated in descendants of the exposed cells through two mitotic divisions before fading from the population. In both haploid starved cells and diploid produced meiotic spores we show that their cellular memories are not attributable to trehalose, a widely regarded stress protectant that accumulates in these cell types. Moreover, the transiently heritable stress resistant state induced by glucose starvation in haploid cells is independent of the Msn2/4 transcription factors, which are known to program cellular memory induced by exposure of cells to NaCl. Our findings identify new developmentally and nutritionally induced states of cellular memory that exhibit striking degrees of persistence and mitotic heritability. |
| format | Article |
| id | doaj-art-817634a8ee1c4e0196ce2e9ff285722f |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2018-10-01 |
| publisher | Shared Science Publishers OG |
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| series | Microbial Cell |
| spelling | doaj-art-817634a8ee1c4e0196ce2e9ff285722f2025-08-20T02:52:37ZengShared Science Publishers OGMicrobial Cell2311-26382018-10-0151151152110.15698/mic2018.11.657Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeastHeldder Gutierrez0Bakhtiyar Taghizada1Marc D. Meneghini2Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.Transient exposures to environmental stresses induce altered physiological states in exposed cells that persist after the stresses have been removed. These states, referred to as cellular memory, can even be passed on to daughter cells and may thus be thought of as embodying a form of epigenetic inheritance. We find that meiotically produced spores in the budding yeast S. cerevisiae possess a state of heightened stress resistance that, following their germination, persists for numerous mitotic generations. As yeast meiotic development is essentially a starvation response that a/alpha diploid cells engage, we sought to model this phenomenon by subjecting haploid cells to starvation conditions. We find also that haploid cells exposed to glucose withdrawal acquire a state of elevated stress resistance that persists after the reintroduction of these cells to glucose-replete media. Following release from lengthy durations of glucose starvation, we confirm that this physiological state of enhanced stress resistance is propagated in descendants of the exposed cells through two mitotic divisions before fading from the population. In both haploid starved cells and diploid produced meiotic spores we show that their cellular memories are not attributable to trehalose, a widely regarded stress protectant that accumulates in these cell types. Moreover, the transiently heritable stress resistant state induced by glucose starvation in haploid cells is independent of the Msn2/4 transcription factors, which are known to program cellular memory induced by exposure of cells to NaCl. Our findings identify new developmentally and nutritionally induced states of cellular memory that exhibit striking degrees of persistence and mitotic heritability.http://microbialcell.com/researcharticles/nutritional-and-meiotic-induction-of-transiently-heritable-stress-resistant-states-in-budding-yeast/Cellular memorysporulationgerminationglucose starvationNDT80TPS1yeaststress resistanceMSN2MSN4 |
| spellingShingle | Heldder Gutierrez Bakhtiyar Taghizada Marc D. Meneghini Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast Microbial Cell Cellular memory sporulation germination glucose starvation NDT80 TPS1 yeast stress resistance MSN2 MSN4 |
| title | Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast |
| title_full | Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast |
| title_fullStr | Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast |
| title_full_unstemmed | Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast |
| title_short | Nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast |
| title_sort | nutritional and meiotic induction of transiently heritable stress resistant states in budding yeast |
| topic | Cellular memory sporulation germination glucose starvation NDT80 TPS1 yeast stress resistance MSN2 MSN4 |
| url | http://microbialcell.com/researcharticles/nutritional-and-meiotic-induction-of-transiently-heritable-stress-resistant-states-in-budding-yeast/ |
| work_keys_str_mv | AT helddergutierrez nutritionalandmeioticinductionoftransientlyheritablestressresistantstatesinbuddingyeast AT bakhtiyartaghizada nutritionalandmeioticinductionoftransientlyheritablestressresistantstatesinbuddingyeast AT marcdmeneghini nutritionalandmeioticinductionoftransientlyheritablestressresistantstatesinbuddingyeast |