A new bicornuate model of rat uterus transplantation
Abstract Introduction Uterus transplantation has revolutionized reproductive medicine for women with absolute uterine factor infertility, resulting in more than 40 reported successful live births worldwide to date. Small animal models are pivotal to refine this surgical and immunological challenging...
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| Format: | Article |
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Wiley
2025-03-01
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| Series: | Acta Obstetricia et Gynecologica Scandinavica |
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| Online Access: | https://doi.org/10.1111/aogs.14857 |
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| author | Dietrich Polenz Igor Maximilian Sauer Friederike Martin Anja Reutzel‐Selke Muhammad Imtiaz Ashraf Anja Schirmeier Steffen Lippert Kirsten Führer Johann Pratschke Stefan Günther Tullius Simon Moosburner |
| author_facet | Dietrich Polenz Igor Maximilian Sauer Friederike Martin Anja Reutzel‐Selke Muhammad Imtiaz Ashraf Anja Schirmeier Steffen Lippert Kirsten Führer Johann Pratschke Stefan Günther Tullius Simon Moosburner |
| author_sort | Dietrich Polenz |
| collection | DOAJ |
| description | Abstract Introduction Uterus transplantation has revolutionized reproductive medicine for women with absolute uterine factor infertility, resulting in more than 40 reported successful live births worldwide to date. Small animal models are pivotal to refine this surgical and immunological challenging procedure aiming to enhance safety for both the mother and the child. Material and Methods We established a syngeneic bicornuate uterus transplantation model in young female Lewis rats. All surgical procedures were conducted by an experienced and skilled microsurgeon who organized the learning process into multiple structured steps. Animals underwent meticulous preoperative preparation and postoperative care. Transplant success was monitored by sequential biopsies, monitoring graft viability and documenting histological changes long‐term. Results Bicornuate uterus transplantation were successfully established achieving an over 70% graft survival rate with the passage of time. The bicornuate model demonstrated safety and feasibility, yielding outcomes comparable to the unicornuate model in terms of ischemia times and complications. Longitudinal biopsies were well‐tolerated, enabling comprehensive monitoring throughout the study. Conclusions Our novel bicornuate rat uterus transplantation model provides a distinctive opportunity for sequential biopsies at various intervals after transplantation and, therefore, comprehensive monitoring of graft health, viability, and identification of potential signs of rejection. Furthermore, this model allows for different interventions in each horn for comparative studies without interobserver differences contrary to the established unicornuate model. By closely replicating the clinical setting, this model stands as a valuable tool for ongoing research in the field of uterus transplantation, promoting further innovation and deeper insights into the intricacies of the uterus transplant procedure. |
| format | Article |
| id | doaj-art-816f6a7021104fd0adc8e32cd4e42596 |
| institution | Kabale University |
| issn | 0001-6349 1600-0412 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Acta Obstetricia et Gynecologica Scandinavica |
| spelling | doaj-art-816f6a7021104fd0adc8e32cd4e425962025-08-20T03:31:00ZengWileyActa Obstetricia et Gynecologica Scandinavica0001-63491600-04122025-03-01104345246010.1111/aogs.14857A new bicornuate model of rat uterus transplantationDietrich Polenz0Igor Maximilian Sauer1Friederike Martin2Anja Reutzel‐Selke3Muhammad Imtiaz Ashraf4Anja Schirmeier5Steffen Lippert6Kirsten Führer7Johann Pratschke8Stefan Günther Tullius9Simon Moosburner10Department of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyDivision of Transplant Surgery, Department of Surgery, Brigham and Women's Hospital Harvard Medical School Boston Massachusetts USADepartment of Surgery, Experimental Surgery Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt‐Universität zu Berlin Berlin GermanyAbstract Introduction Uterus transplantation has revolutionized reproductive medicine for women with absolute uterine factor infertility, resulting in more than 40 reported successful live births worldwide to date. Small animal models are pivotal to refine this surgical and immunological challenging procedure aiming to enhance safety for both the mother and the child. Material and Methods We established a syngeneic bicornuate uterus transplantation model in young female Lewis rats. All surgical procedures were conducted by an experienced and skilled microsurgeon who organized the learning process into multiple structured steps. Animals underwent meticulous preoperative preparation and postoperative care. Transplant success was monitored by sequential biopsies, monitoring graft viability and documenting histological changes long‐term. Results Bicornuate uterus transplantation were successfully established achieving an over 70% graft survival rate with the passage of time. The bicornuate model demonstrated safety and feasibility, yielding outcomes comparable to the unicornuate model in terms of ischemia times and complications. Longitudinal biopsies were well‐tolerated, enabling comprehensive monitoring throughout the study. Conclusions Our novel bicornuate rat uterus transplantation model provides a distinctive opportunity for sequential biopsies at various intervals after transplantation and, therefore, comprehensive monitoring of graft health, viability, and identification of potential signs of rejection. Furthermore, this model allows for different interventions in each horn for comparative studies without interobserver differences contrary to the established unicornuate model. By closely replicating the clinical setting, this model stands as a valuable tool for ongoing research in the field of uterus transplantation, promoting further innovation and deeper insights into the intricacies of the uterus transplant procedure.https://doi.org/10.1111/aogs.14857bicornuate uterusratuterus transplantation |
| spellingShingle | Dietrich Polenz Igor Maximilian Sauer Friederike Martin Anja Reutzel‐Selke Muhammad Imtiaz Ashraf Anja Schirmeier Steffen Lippert Kirsten Führer Johann Pratschke Stefan Günther Tullius Simon Moosburner A new bicornuate model of rat uterus transplantation Acta Obstetricia et Gynecologica Scandinavica bicornuate uterus rat uterus transplantation |
| title | A new bicornuate model of rat uterus transplantation |
| title_full | A new bicornuate model of rat uterus transplantation |
| title_fullStr | A new bicornuate model of rat uterus transplantation |
| title_full_unstemmed | A new bicornuate model of rat uterus transplantation |
| title_short | A new bicornuate model of rat uterus transplantation |
| title_sort | new bicornuate model of rat uterus transplantation |
| topic | bicornuate uterus rat uterus transplantation |
| url | https://doi.org/10.1111/aogs.14857 |
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