Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form
Prebiotic isomaltooligosaccharides (IMOs) are composed of α-d-glucose units linked by glucosidic α (1Ø6) bonds and they remain intact until reaching the large bowels, where they are metabolized by bifidobacteria and lactobacillus. These saccharides can also be synthesized enzymatically, wherein the...
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SAGE Publishing
2012-09-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/0263-6174.30.8-9.773 |
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| author | M.C. Rabelo C.S.M. Pereira S. Rodrigues A.E. Rodrigues D.C.S. Azevedo |
| author_facet | M.C. Rabelo C.S.M. Pereira S. Rodrigues A.E. Rodrigues D.C.S. Azevedo |
| author_sort | M.C. Rabelo |
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| description | Prebiotic isomaltooligosaccharides (IMOs) are composed of α-d-glucose units linked by glucosidic α (1Ø6) bonds and they remain intact until reaching the large bowels, where they are metabolized by bifidobacteria and lactobacillus. These saccharides can also be synthesized enzymatically, wherein the reaction media is composed of monosaccharides (glucose and fructose), disaccharides (sucrose and maltose), IMOs (the product of interest) and dextrans. Dextrans are readily separated by solvent precipitation; however, continuous chromatographic separation (simulated moving bed or SMB) should be an interesting option for the enrichment and purification of IMOs from mono- and disaccharides. The objective of the present work was to measure fundamental adsorption data of IMOs, mono- and disaccharides in preparative columns packed with ion-exchange resin of different cationic forms (K + , Ca + , H + and Na + ), so as to verify the influence of the cation on the selectivity and capacity. Ion exchange in H + form showed the best performance in terms of selectivity between maltose and maltotriose, but the resin in the K + form had superior adsorption capacities. Additionally, on the basis of equilibrium theory, optimal SMB operating conditions for resin H + will yield the highest productivity as compared with the other cationic forms. |
| format | Article |
| id | doaj-art-8162fba9839c428a8fcf437e8a376b2c |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2012-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-8162fba9839c428a8fcf437e8a376b2c2025-01-03T01:23:10ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382012-09-013010.1260/0263-6174.30.8-9.773Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic FormM.C. Rabelo0C.S.M. Pereira1S. Rodrigues2A.E. Rodrigues3D.C.S. Azevedo4 Grupo de Pesquisa em Separações por Adsorção (GPSA), Department of Chemical Engineering, Universidade Federal do Ceará, Campus do Pici, Fortaleza, Brazil Laboratory of Separation and Reaction Engineering (LSRE), Associate Laboratory LSRE/LCM, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal Department of Food Engineering, Universidade Federal do Ceará, Campus do Pici, Fortaleza, Brazil Laboratory of Separation and Reaction Engineering (LSRE), Associate Laboratory LSRE/LCM, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal Grupo de Pesquisa em Separações por Adsorção (GPSA), Department of Chemical Engineering, Universidade Federal do Ceará, Campus do Pici, Fortaleza, BrazilPrebiotic isomaltooligosaccharides (IMOs) are composed of α-d-glucose units linked by glucosidic α (1Ø6) bonds and they remain intact until reaching the large bowels, where they are metabolized by bifidobacteria and lactobacillus. These saccharides can also be synthesized enzymatically, wherein the reaction media is composed of monosaccharides (glucose and fructose), disaccharides (sucrose and maltose), IMOs (the product of interest) and dextrans. Dextrans are readily separated by solvent precipitation; however, continuous chromatographic separation (simulated moving bed or SMB) should be an interesting option for the enrichment and purification of IMOs from mono- and disaccharides. The objective of the present work was to measure fundamental adsorption data of IMOs, mono- and disaccharides in preparative columns packed with ion-exchange resin of different cationic forms (K + , Ca + , H + and Na + ), so as to verify the influence of the cation on the selectivity and capacity. Ion exchange in H + form showed the best performance in terms of selectivity between maltose and maltotriose, but the resin in the K + form had superior adsorption capacities. Additionally, on the basis of equilibrium theory, optimal SMB operating conditions for resin H + will yield the highest productivity as compared with the other cationic forms.https://doi.org/10.1260/0263-6174.30.8-9.773 |
| spellingShingle | M.C. Rabelo C.S.M. Pereira S. Rodrigues A.E. Rodrigues D.C.S. Azevedo Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form Adsorption Science & Technology |
| title | Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form |
| title_full | Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form |
| title_fullStr | Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form |
| title_full_unstemmed | Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form |
| title_short | Chromatographic Separation of Isomaltooligosaccharides on Ion-Exchange Resins: Effect of the Cationic Form |
| title_sort | chromatographic separation of isomaltooligosaccharides on ion exchange resins effect of the cationic form |
| url | https://doi.org/10.1260/0263-6174.30.8-9.773 |
| work_keys_str_mv | AT mcrabelo chromatographicseparationofisomaltooligosaccharidesonionexchangeresinseffectofthecationicform AT csmpereira chromatographicseparationofisomaltooligosaccharidesonionexchangeresinseffectofthecationicform AT srodrigues chromatographicseparationofisomaltooligosaccharidesonionexchangeresinseffectofthecationicform AT aerodrigues chromatographicseparationofisomaltooligosaccharidesonionexchangeresinseffectofthecationicform AT dcsazevedo chromatographicseparationofisomaltooligosaccharidesonionexchangeresinseffectofthecationicform |