Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells
Tumor necrosis factor-α (TNF-α ) plays an important role in the pathogenesis of rheumatoid arthritis. The present study was to evaluate the effects of lipopolysaccharide (LPS), phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells (AMC). T...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350123139 |
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| _version_ | 1850215951387066368 |
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| author | Ming-Yuh Shiau Hui-Ling Chiou Yao-Ling Lee Tzer-Min Kuo Yih-Hsin Chang |
| author_facet | Ming-Yuh Shiau Hui-Ling Chiou Yao-Ling Lee Tzer-Min Kuo Yih-Hsin Chang |
| author_sort | Ming-Yuh Shiau |
| collection | DOAJ |
| description | Tumor necrosis factor-α (TNF-α ) plays an important role in the pathogenesis of rheumatoid arthritis. The present study was to evaluate the effects of lipopolysaccharide (LPS), phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells (AMC). TNF-α cytotoxicity in LPS-treated, phytomitogen-treated, and cytodifferentiation agent-treated AMC supernatants were analyzed by the L929 bioassay system. Our results showed that LPS could induce homogeneous TNF-α production by AMC whereas, in addition to TNF-α, phytomitogens could also induce other TNF-like factors. Neither methotrexate, retinoic acid nor sodium butyrate can inhibit TNF-α cytotoxicity, while hexamethylene bisacetamide could not only inhibit TNF-α cytotoxicity but also TNF-α inducing ability of LPS to AMC. |
| format | Article |
| id | doaj-art-81606594182d4c08a9d73ac55a1ab310 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-81606594182d4c08a9d73ac55a1ab3102025-08-20T02:08:27ZengWileyMediators of Inflammation0962-93511466-18612001-01-0110419920810.1080/09629350123139Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cellsMing-Yuh Shiau0Hui-Ling Chiou1Yao-Ling Lee2Tzer-Min Kuo3Yih-Hsin Chang4Hung Kuang Institute of Technology, Chung Shan Medical University, Taichung 402, TaiwanSchool of Medical Technology, Chung Shan Medical University, Taichung 402, TaiwanSchool of Medical Technology, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Immunology, Chung Shan Medical University, Taichung 402, TaiwanSchool of Medical Technology, Chung Shan Medical University, Taichung 402, TaiwanTumor necrosis factor-α (TNF-α ) plays an important role in the pathogenesis of rheumatoid arthritis. The present study was to evaluate the effects of lipopolysaccharide (LPS), phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells (AMC). TNF-α cytotoxicity in LPS-treated, phytomitogen-treated, and cytodifferentiation agent-treated AMC supernatants were analyzed by the L929 bioassay system. Our results showed that LPS could induce homogeneous TNF-α production by AMC whereas, in addition to TNF-α, phytomitogens could also induce other TNF-like factors. Neither methotrexate, retinoic acid nor sodium butyrate can inhibit TNF-α cytotoxicity, while hexamethylene bisacetamide could not only inhibit TNF-α cytotoxicity but also TNF-α inducing ability of LPS to AMC.http://dx.doi.org/10.1080/09629350123139 |
| spellingShingle | Ming-Yuh Shiau Hui-Ling Chiou Yao-Ling Lee Tzer-Min Kuo Yih-Hsin Chang Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells Mediators of Inflammation |
| title | Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells |
| title_full | Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells |
| title_fullStr | Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells |
| title_full_unstemmed | Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells |
| title_short | Establishment of a consistent L929 bioassay system for TNF-α quantitation to evaluate the effect of lipopolysaccharide, phytomitogens and cytodifferentiation agents on cytotoxicity of TNF-α secreted by adherent human mononuclear cells |
| title_sort | establishment of a consistent l929 bioassay system for tnf α quantitation to evaluate the effect of lipopolysaccharide phytomitogens and cytodifferentiation agents on cytotoxicity of tnf α secreted by adherent human mononuclear cells |
| url | http://dx.doi.org/10.1080/09629350123139 |
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