A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.
<h4>Background</h4>Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules re...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-04-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004581&type=printable |
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| author | Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana |
| author_facet | Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana |
| author_sort | Paula Beatriz Santiago |
| collection | DOAJ |
| description | <h4>Background</h4>Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses.<h4>Methods/principal findings</h4>Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant.<h4>Conclusions/significance</h4>The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. |
| format | Article |
| id | doaj-art-815d7ada8d9043179ba08081f0891b9c |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2016-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-815d7ada8d9043179ba08081f0891b9c2025-08-20T02:03:23ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-04-01104e000458110.1371/journal.pntd.0004581A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.Paula Beatriz SantiagoTeresa C F AssumpçãoCarla Nunes de AraújoIzabela Marques Dourado BastosDavid NevesIonizete Garcia da SilvaSébastien CharneauRayner Myr L QueirozTainá RaiolJoão Victor de Araújo OliveiraMarcelo Valle de SousaEric CalvoJosé M C RibeiroJaime M Santana<h4>Background</h4>Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses.<h4>Methods/principal findings</h4>Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant.<h4>Conclusions/significance</h4>The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004581&type=printable |
| spellingShingle | Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. PLoS Neglected Tropical Diseases |
| title | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_full | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_fullStr | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_full_unstemmed | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_short | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_sort | deep insight into the sialome of rhodnius neglectus a vector of chagas disease |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004581&type=printable |
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