Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt
Bacterial wilt, caused by <i>Ralstonia solanacearum</i>, poses a significant threat to Solanaceae crops, including potatoes. Traditionally, chemical bactericides have been the primary method of disease management. However, the excessive and repeated use of these chemicals has led to the...
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MDPI AG
2025-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/11/3/257 |
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| author | Kamel M. Elhalag Yasser S. A. Mazrou Abdelnaser A. Elzaawely Abeer H. Makhlouf Sherine A. Elsaed Hanafey F. Maswada Tran Dang Xuan Asmaa El-Nagar |
| author_facet | Kamel M. Elhalag Yasser S. A. Mazrou Abdelnaser A. Elzaawely Abeer H. Makhlouf Sherine A. Elsaed Hanafey F. Maswada Tran Dang Xuan Asmaa El-Nagar |
| author_sort | Kamel M. Elhalag |
| collection | DOAJ |
| description | Bacterial wilt, caused by <i>Ralstonia solanacearum</i>, poses a significant threat to Solanaceae crops, including potatoes. Traditionally, chemical bactericides have been the primary method of disease management. However, the excessive and repeated use of these chemicals has led to the emergence of resistant <i>R. solanacearum</i> strains and raised environmental and safety concerns. Therefore, the current study aimed to explore bacteriophages as a sustainable and eco-friendly alternative to chemical control. Specifically, we evaluated the efficacy of the bacteriophage RsPod1EGY as a biocontrol agent under greenhouse conditions to reduce disease severity and maintain potato productivity. To improve phage survival and activity, we tested six carrier types; compost, peat moss, clay soil, sandy soil, talc powder, and wheat bran. Our findings indicated that compost, talc powder, and wheat bran significantly prolonged the phage’s viability and activity, reducing disease severity by 90%, compared to 65% and 85% for peat moss and phage-alone treatments respectively. In contrast, pathogen control treatments resulted in complete plant mortality. Phage survival was enhanced in pathogen-infected soils, supporting its role in reducing <i>R. solanacearum</i> populations. Potato yield was preserved in compost and talc powder treatments comparable to pathogen-free controls. These results underscore the potential of integrating bacteriophage RsPod1EGY with suitable carriers for sustainable bacterial wilt management, highlighting the importance of delivery methods in agricultural applications. |
| format | Article |
| id | doaj-art-815507e251484eec89f501976e11d41b |
| institution | OA Journals |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-815507e251484eec89f501976e11d41b2025-08-20T02:11:11ZengMDPI AGHorticulturae2311-75242025-02-0111325710.3390/horticulturae11030257Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial WiltKamel M. Elhalag0Yasser S. A. Mazrou1Abdelnaser A. Elzaawely2Abeer H. Makhlouf3Sherine A. Elsaed4Hanafey F. Maswada5Tran Dang Xuan6Asmaa El-Nagar7Bacterial Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza 12619, EgyptApplied College, King Khalid University, Abha 62587, Saudi ArabiaDepartment of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31527, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Minufiya University, Shibin El-Kom 32511, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31527, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31527, EgyptCenter for the Planetary Health and Innovation Science, The IDEC Institute, Hiroshima University, Hiroshima City 739-8529, Hiroshima, JapanDepartment of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31527, EgyptBacterial wilt, caused by <i>Ralstonia solanacearum</i>, poses a significant threat to Solanaceae crops, including potatoes. Traditionally, chemical bactericides have been the primary method of disease management. However, the excessive and repeated use of these chemicals has led to the emergence of resistant <i>R. solanacearum</i> strains and raised environmental and safety concerns. Therefore, the current study aimed to explore bacteriophages as a sustainable and eco-friendly alternative to chemical control. Specifically, we evaluated the efficacy of the bacteriophage RsPod1EGY as a biocontrol agent under greenhouse conditions to reduce disease severity and maintain potato productivity. To improve phage survival and activity, we tested six carrier types; compost, peat moss, clay soil, sandy soil, talc powder, and wheat bran. Our findings indicated that compost, talc powder, and wheat bran significantly prolonged the phage’s viability and activity, reducing disease severity by 90%, compared to 65% and 85% for peat moss and phage-alone treatments respectively. In contrast, pathogen control treatments resulted in complete plant mortality. Phage survival was enhanced in pathogen-infected soils, supporting its role in reducing <i>R. solanacearum</i> populations. Potato yield was preserved in compost and talc powder treatments comparable to pathogen-free controls. These results underscore the potential of integrating bacteriophage RsPod1EGY with suitable carriers for sustainable bacterial wilt management, highlighting the importance of delivery methods in agricultural applications.https://www.mdpi.com/2311-7524/11/3/257bacterial wiltbacteriophagebiological controlcarriers<i>Ralstonia solanacearum</i> |
| spellingShingle | Kamel M. Elhalag Yasser S. A. Mazrou Abdelnaser A. Elzaawely Abeer H. Makhlouf Sherine A. Elsaed Hanafey F. Maswada Tran Dang Xuan Asmaa El-Nagar Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt Horticulturae bacterial wilt bacteriophage biological control carriers <i>Ralstonia solanacearum</i> |
| title | Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt |
| title_full | Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt |
| title_fullStr | Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt |
| title_full_unstemmed | Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt |
| title_short | Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt |
| title_sort | carrier based application of rspod1egy phage to effective control potato bacterial wilt |
| topic | bacterial wilt bacteriophage biological control carriers <i>Ralstonia solanacearum</i> |
| url | https://www.mdpi.com/2311-7524/11/3/257 |
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