The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats
Abstract Background Epidural fibrosis (EF) is the major complication that develops in the operative region of the spinal vertebrae. This fibrous scar connects the connective tissue around the lateral nerve roots and epidural, resulting in severe pain post-spinal operation and impairment of the nerve...
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BMC
2025-08-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02951-3 |
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| author | Samah Fouad Marwa Abass Awad Rizk Esam Mosbah Mostafa M. Nabeeh Ayman S. Elmezayyen M. I. El-Henawey Adel Zaghloul |
| author_facet | Samah Fouad Marwa Abass Awad Rizk Esam Mosbah Mostafa M. Nabeeh Ayman S. Elmezayyen M. I. El-Henawey Adel Zaghloul |
| author_sort | Samah Fouad |
| collection | DOAJ |
| description | Abstract Background Epidural fibrosis (EF) is the major complication that develops in the operative region of the spinal vertebrae. This fibrous scar connects the connective tissue around the lateral nerve roots and epidural, resulting in severe pain post-spinal operation and impairment of the nerves’ function. Purpose This study was conducted to investigate the effect of using platelet-rich fibrin (PRF), chitosan, and their combination in reducing epidural fibrosis after laminectomy in rats. Methods Ninety male Sprague Dawley rats weighing 255 ± 55 g were randomly assigned to five groups, each consisting of 15 rats: the normal group (control), the laminectomy group, the PRF group, the chitosan group, the combination group (PRF/chitosan), and the donor group. All rats, except the control group, had lumbar laminectomy surgeries between L3 and L5. Macroscopic analysis, histological evaluations, and mRNA analysis for TGFβ-1 and IL6 were compared statistically after a 30-day follow-up. Results In comparison to the laminectomy group, the EF area was significantly decreased in the PRF, chitosan, and combination groups. Histological study, macroscopic inspection, and mRNA expression of TGFβ-1 (P < 0.0001) and IL6 (P < 0.0001) show that the use of PRF with chitosan topically in dura following laminectomy resulted in a decrease in scar tissue formation, inflammation, and EF post-laminectomy. Conclusion The combination of chitosan with PRF is a potential therapeutic approach for minimizing EF in rats after laminectomy. Graphical Abstract |
| format | Article |
| id | doaj-art-814baefe1ece40b9953c852c3b823cba |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-814baefe1ece40b9953c852c3b823cba2025-08-20T03:42:30ZengBMCEuropean Journal of Medical Research2047-783X2025-08-0130111310.1186/s40001-025-02951-3The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in ratsSamah Fouad0Marwa Abass1Awad Rizk2Esam Mosbah3Mostafa M. Nabeeh4Ayman S. Elmezayyen5M. I. El-Henawey6Adel Zaghloul7Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Neurosurgery, Faculty of Medicine, Mansoura UniversityPhysics Department, Faculty of Science, Mansoura UniversityPhysics Department, Faculty of Science, Mansoura UniversityDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityAbstract Background Epidural fibrosis (EF) is the major complication that develops in the operative region of the spinal vertebrae. This fibrous scar connects the connective tissue around the lateral nerve roots and epidural, resulting in severe pain post-spinal operation and impairment of the nerves’ function. Purpose This study was conducted to investigate the effect of using platelet-rich fibrin (PRF), chitosan, and their combination in reducing epidural fibrosis after laminectomy in rats. Methods Ninety male Sprague Dawley rats weighing 255 ± 55 g were randomly assigned to five groups, each consisting of 15 rats: the normal group (control), the laminectomy group, the PRF group, the chitosan group, the combination group (PRF/chitosan), and the donor group. All rats, except the control group, had lumbar laminectomy surgeries between L3 and L5. Macroscopic analysis, histological evaluations, and mRNA analysis for TGFβ-1 and IL6 were compared statistically after a 30-day follow-up. Results In comparison to the laminectomy group, the EF area was significantly decreased in the PRF, chitosan, and combination groups. Histological study, macroscopic inspection, and mRNA expression of TGFβ-1 (P < 0.0001) and IL6 (P < 0.0001) show that the use of PRF with chitosan topically in dura following laminectomy resulted in a decrease in scar tissue formation, inflammation, and EF post-laminectomy. Conclusion The combination of chitosan with PRF is a potential therapeutic approach for minimizing EF in rats after laminectomy. Graphical Abstracthttps://doi.org/10.1186/s40001-025-02951-3ChitosanPlatelets-rich fibrinLaminectomyEpidural fibrosis |
| spellingShingle | Samah Fouad Marwa Abass Awad Rizk Esam Mosbah Mostafa M. Nabeeh Ayman S. Elmezayyen M. I. El-Henawey Adel Zaghloul The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats European Journal of Medical Research Chitosan Platelets-rich fibrin Laminectomy Epidural fibrosis |
| title | The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats |
| title_full | The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats |
| title_fullStr | The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats |
| title_full_unstemmed | The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats |
| title_short | The augmentative role of chitosan and platelet-rich fibrin in reducing epidural fibrosis after laminectomy in rats |
| title_sort | augmentative role of chitosan and platelet rich fibrin in reducing epidural fibrosis after laminectomy in rats |
| topic | Chitosan Platelets-rich fibrin Laminectomy Epidural fibrosis |
| url | https://doi.org/10.1186/s40001-025-02951-3 |
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