Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores
Background. Morphological characterization of hemodialysis membranes is necessary to improve pore design. Aim. To delineate membrane pore structure of a high flux filter, Polyflux 210H. Methods. We used a Joel JSM-6010LV scanning electron microscope (SEM) and a SU6600 Hitachi field emission scanning...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | International Journal of Nephrology |
| Online Access: | http://dx.doi.org/10.1155/2012/304135 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849410479685894144 |
|---|---|
| author | A. Hedayat J. Szpunar N. A. P. Kiran Kumar R. Peace H. Elmoselhi A. Shoker |
| author_facet | A. Hedayat J. Szpunar N. A. P. Kiran Kumar R. Peace H. Elmoselhi A. Shoker |
| author_sort | A. Hedayat |
| collection | DOAJ |
| description | Background. Morphological characterization of hemodialysis membranes is necessary to improve pore design. Aim. To delineate membrane pore structure of a high flux filter, Polyflux 210H. Methods. We used a Joel JSM-6010LV scanning electron microscope (SEM) and a SU6600 Hitachi field emission scanning electron microscope (FESEM) to characterize the pore and fiber morphology. The maximal diameters of selected uremic toxins were calculated using the macromolecular modeling Crystallographic Object-Oriented Toolkit (COOT) software. Results. The mean pore densities on the outermost and innermost surfaces of the membrane were 36.81% and 5.45%, respectively. The membrane exhibited a tortuous structure with poor connection between the inner and outer pores. The aperture’s width in the inner surface ranged between 34 and 45 nm, which is 8.76–11.60 times larger than the estimated maximum diameter of β2-microglobulin (3.88 nm). Conclusion. The results suggest that the diameter size of inner pore apertures is not a limiting factor to middle molecules clearance, the extremely diminished density is. Increasing inner pore density and improving channel structure are strategies to improve clearance of middle molecules. |
| format | Article |
| id | doaj-art-8149d3f85657471e90eb849cae681de7 |
| institution | Kabale University |
| issn | 2090-214X 2090-2158 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Nephrology |
| spelling | doaj-art-8149d3f85657471e90eb849cae681de72025-08-20T03:35:06ZengWileyInternational Journal of Nephrology2090-214X2090-21582012-01-01201210.1155/2012/304135304135Morphological Characterization of the Polyflux 210H Hemodialysis Filter PoresA. Hedayat0J. Szpunar1N. A. P. Kiran Kumar2R. Peace3H. Elmoselhi4A. Shoker5College of Dentistry, University of Saskatchewan, 105 Wiggins Road, Saskatoon, SK, S7N 5E4, CanadaDepartment of Mechanical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK, S7N 5A9, CanadaDepartment of Mechanical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK, S7N 5A9, CanadaDepartment of Mechanical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK, S7N 5A9, CanadaSaskatchewan Transplant Program, St. Paul’s Hospital, 1702, 20th Street West, Saskatoon, SK, S7M 0Z9, CanadaSaskatchewan Transplant Program, St. Paul’s Hospital, 1702, 20th Street West, Saskatoon, SK, S7M 0Z9, CanadaBackground. Morphological characterization of hemodialysis membranes is necessary to improve pore design. Aim. To delineate membrane pore structure of a high flux filter, Polyflux 210H. Methods. We used a Joel JSM-6010LV scanning electron microscope (SEM) and a SU6600 Hitachi field emission scanning electron microscope (FESEM) to characterize the pore and fiber morphology. The maximal diameters of selected uremic toxins were calculated using the macromolecular modeling Crystallographic Object-Oriented Toolkit (COOT) software. Results. The mean pore densities on the outermost and innermost surfaces of the membrane were 36.81% and 5.45%, respectively. The membrane exhibited a tortuous structure with poor connection between the inner and outer pores. The aperture’s width in the inner surface ranged between 34 and 45 nm, which is 8.76–11.60 times larger than the estimated maximum diameter of β2-microglobulin (3.88 nm). Conclusion. The results suggest that the diameter size of inner pore apertures is not a limiting factor to middle molecules clearance, the extremely diminished density is. Increasing inner pore density and improving channel structure are strategies to improve clearance of middle molecules.http://dx.doi.org/10.1155/2012/304135 |
| spellingShingle | A. Hedayat J. Szpunar N. A. P. Kiran Kumar R. Peace H. Elmoselhi A. Shoker Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores International Journal of Nephrology |
| title | Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores |
| title_full | Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores |
| title_fullStr | Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores |
| title_full_unstemmed | Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores |
| title_short | Morphological Characterization of the Polyflux 210H Hemodialysis Filter Pores |
| title_sort | morphological characterization of the polyflux 210h hemodialysis filter pores |
| url | http://dx.doi.org/10.1155/2012/304135 |
| work_keys_str_mv | AT ahedayat morphologicalcharacterizationofthepolyflux210hhemodialysisfilterpores AT jszpunar morphologicalcharacterizationofthepolyflux210hhemodialysisfilterpores AT napkirankumar morphologicalcharacterizationofthepolyflux210hhemodialysisfilterpores AT rpeace morphologicalcharacterizationofthepolyflux210hhemodialysisfilterpores AT helmoselhi morphologicalcharacterizationofthepolyflux210hhemodialysisfilterpores AT ashoker morphologicalcharacterizationofthepolyflux210hhemodialysisfilterpores |