Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River
To provide a general overview of the microbial communities in environmentally conserved estuaries, the top 5 cm of sediment was sampled from the sandy estuary of the Shirakawa River and from the muddy estuary of the Midorikawa River. Higher amounts of organic matter were detected in the Midorikawa e...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2016-12-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/208 |
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| author | Tran Thanh Liem Mitsuaki Nakano Hiroto Ohta Takuro Niidome Tatsuya Masuda Kiyoshi Takikawa Shigeru Morimura |
| author_facet | Tran Thanh Liem Mitsuaki Nakano Hiroto Ohta Takuro Niidome Tatsuya Masuda Kiyoshi Takikawa Shigeru Morimura |
| author_sort | Tran Thanh Liem |
| collection | DOAJ |
| description | To provide a general overview of the microbial communities in environmentally conserved estuaries, the top 5 cm of sediment was sampled from the sandy estuary of the Shirakawa River and from the muddy estuary of the Midorikawa River. Higher amounts of organic matter were detected in the Midorikawa estuary sample than in the Shirakawa estuary sample. Measurement of redox potential revealed that the Shirakawa estuary was aerobic and the Midorikawa estuary was much less aerobic. Clone analysis was performed by targeting partial 16S rRNA gene sequences and using extracted DNA from the samples as a template. Various bacteria were detected, among which Gammaproteobacteria was dominant at both estuaries. Unclassified clones were detected in the Gammaproteobacteria group, mainly among samples from the Midorikawa estuary. Other detected bacterial groups were Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, Actinobacteria, and Bacteroidetes. All the Deltaproteobacteria clones were anaerobic sulfate-reducing bacteria. Those aerobic and anaerobic bacteria coexisted in the top 5 cm of the estuary sediments indicating the surface layer have active sulfur and carbon cycle. Abundance of aerobic Gammaproteobacteria may be an indicator for conserved estuaries. |
| format | Article |
| id | doaj-art-813fe1b6882348cb8cb2b8ac6ad841db |
| institution | Kabale University |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-813fe1b6882348cb8cb2b8ac6ad841db2025-08-20T03:47:33ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552016-12-01626370https://doi.org/10.21776/ub.jels.2016.006.02.02Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa RiverTran Thanh Liem0Mitsuaki Nakano1Hiroto Ohta2Takuro Niidome3Tatsuya Masuda4Kiyoshi Takikawa5Shigeru Morimura6Kumamoto University, Kumamoto City, JapanKumamoto University, Kumamoto City, JapanKumamoto University, Kumamoto City, JapanKumamoto University, Kumamoto City, JapanKumamoto University, Kumamoto City, JapanKumamoto University, Kumamoto City, JapanKumamoto University, Kumamoto City, JapanTo provide a general overview of the microbial communities in environmentally conserved estuaries, the top 5 cm of sediment was sampled from the sandy estuary of the Shirakawa River and from the muddy estuary of the Midorikawa River. Higher amounts of organic matter were detected in the Midorikawa estuary sample than in the Shirakawa estuary sample. Measurement of redox potential revealed that the Shirakawa estuary was aerobic and the Midorikawa estuary was much less aerobic. Clone analysis was performed by targeting partial 16S rRNA gene sequences and using extracted DNA from the samples as a template. Various bacteria were detected, among which Gammaproteobacteria was dominant at both estuaries. Unclassified clones were detected in the Gammaproteobacteria group, mainly among samples from the Midorikawa estuary. Other detected bacterial groups were Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, Actinobacteria, and Bacteroidetes. All the Deltaproteobacteria clones were anaerobic sulfate-reducing bacteria. Those aerobic and anaerobic bacteria coexisted in the top 5 cm of the estuary sediments indicating the surface layer have active sulfur and carbon cycle. Abundance of aerobic Gammaproteobacteria may be an indicator for conserved estuaries.https://jels.ub.ac.id/index.php/jels/article/view/208conserved environmentclone analysisestuarymicrobial community16s rrna gene. |
| spellingShingle | Tran Thanh Liem Mitsuaki Nakano Hiroto Ohta Takuro Niidome Tatsuya Masuda Kiyoshi Takikawa Shigeru Morimura Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River Journal of Experimental Life Science conserved environment clone analysis estuary microbial community 16s rrna gene. |
| title | Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River |
| title_full | Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River |
| title_fullStr | Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River |
| title_full_unstemmed | Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River |
| title_short | Microbial Community Composition of Two Environmentally Conserved Estuaries in the Midorikawa River and Shirakawa River |
| title_sort | microbial community composition of two environmentally conserved estuaries in the midorikawa river and shirakawa river |
| topic | conserved environment clone analysis estuary microbial community 16s rrna gene. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/208 |
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