A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.

As a chronic illness derived from hair cells of the inner ear, Menière's disease (MD) negatively influences the quality of life of individuals and leads to a number of symptoms, such as dizziness, temporary hearing loss, and tinnitus. The complete identification of novel genes related to MD wou...

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Main Authors: Lin Li, YanShu Wang, Lifeng An, XiangYin Kong, Tao Huang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182592&type=printable
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author Lin Li
YanShu Wang
Lifeng An
XiangYin Kong
Tao Huang
author_facet Lin Li
YanShu Wang
Lifeng An
XiangYin Kong
Tao Huang
author_sort Lin Li
collection DOAJ
description As a chronic illness derived from hair cells of the inner ear, Menière's disease (MD) negatively influences the quality of life of individuals and leads to a number of symptoms, such as dizziness, temporary hearing loss, and tinnitus. The complete identification of novel genes related to MD would help elucidate its underlying pathological mechanisms and improve its diagnosis and treatment. In this study, a network-based method was developed to identify novel MD-related genes based on known MD-related genes. A human protein-protein interaction (PPI) network was constructed using the PPI information reported in the STRING database. A classic ranking algorithm, the random walk with restart (RWR) algorithm, was employed to search for novel genes using known genes as seed nodes. To make the identified genes more reliable, a series of screening tests, including a permutation test, an interaction test and an enrichment test, were designed to select essential genes from those obtained by the RWR algorithm. As a result, several inferred genes, such as CD4, NOTCH2 and IL6, were discovered. Finally, a detailed biological analysis was performed on fifteen of the important inferred genes, which indicated their strong associations with MD.
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spelling doaj-art-813bcc84d2f141c1804f00a013dab31e2025-08-20T03:04:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018259210.1371/journal.pone.0182592A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.Lin LiYanShu WangLifeng AnXiangYin KongTao HuangAs a chronic illness derived from hair cells of the inner ear, Menière's disease (MD) negatively influences the quality of life of individuals and leads to a number of symptoms, such as dizziness, temporary hearing loss, and tinnitus. The complete identification of novel genes related to MD would help elucidate its underlying pathological mechanisms and improve its diagnosis and treatment. In this study, a network-based method was developed to identify novel MD-related genes based on known MD-related genes. A human protein-protein interaction (PPI) network was constructed using the PPI information reported in the STRING database. A classic ranking algorithm, the random walk with restart (RWR) algorithm, was employed to search for novel genes using known genes as seed nodes. To make the identified genes more reliable, a series of screening tests, including a permutation test, an interaction test and an enrichment test, were designed to select essential genes from those obtained by the RWR algorithm. As a result, several inferred genes, such as CD4, NOTCH2 and IL6, were discovered. Finally, a detailed biological analysis was performed on fifteen of the important inferred genes, which indicated their strong associations with MD.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182592&type=printable
spellingShingle Lin Li
YanShu Wang
Lifeng An
XiangYin Kong
Tao Huang
A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.
PLoS ONE
title A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.
title_full A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.
title_fullStr A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.
title_full_unstemmed A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.
title_short A network-based method using a random walk with restart algorithm and screening tests to identify novel genes associated with Menière's disease.
title_sort network based method using a random walk with restart algorithm and screening tests to identify novel genes associated with meniere s disease
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182592&type=printable
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