Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan
Background: Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae have been reported previously from Pakistan but the genotypic characteristics of these enzymes is not known. Hence the aim of the study was first to characterise the genotypic content of these beta-lactamases and se...
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The Journal of Infection in Developing Countries
2010-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/674 |
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| author | Erum Khan Thamarai Schneiders Afia Zafar Erum Aziz Asra Parekh Rumina Hasan |
| author_facet | Erum Khan Thamarai Schneiders Afia Zafar Erum Aziz Asra Parekh Rumina Hasan |
| author_sort | Erum Khan |
| collection | DOAJ |
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Background: Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae have been reported previously from Pakistan but the genotypic characteristics of these enzymes is not known. Hence the aim of the study was first to characterise the genotypic content of these beta-lactamases and secondly to assess the clonal relationship of these isolates.
Methodology: We analysed 65 non-duplicate ESBL positive, K. pneumoniae isolates prospectively collected based on phenotype as detected using the two-disc method. Isolates were collected from different sources: blood cultures (46.15%; n = 30); tracheal aspirates (24.6%; n = 16); urine (10.7%; n = 7); wound swabs, pus and tissue (18.4%; n = 12). ESBL production was confirmed by the ESBL E-test method and the presence of the blaCTX-M encoding genes was confirmed by polymerase chain reaction. The clonal relationship of clinical isolates was studied by Pulsed Field Gel Electrophoresis.
Results: The results showed that 93.84% (n = 61) isolates of K. pneumoniae were positive for the blaCTX-M-1 group. One isolate showed PCR signals for blaCTX-M-25 group. None of our isolates were positive for CTX-M groups 2, 8 and 9. The majority of blaCTX-M positive isolates were genetically unrelated and no epidemic clones were identified. Conclusion: This study reports the emergence of CTX-M groups 1 and 25 producing isolates of K. pneumoniae with genetic diversity in Karachi, Pakistan.
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| format | Article |
| id | doaj-art-812df3f1b2044b139f234cc06bbbd377 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-812df3f1b2044b139f234cc06bbbd3772025-08-20T02:57:04ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-05-0140810.3855/jidc.674Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, PakistanErum Khan0Thamarai Schneiders1Afia Zafar2Erum Aziz3Asra Parekh4Rumina Hasan5Department of Pathology/Microbiolog, Aga Khan University Hospital, Karachi, PakistanQueen's University Belfast Northern IrelandAga Khan University Hospital, KarachiAga Khan University HospitalAga Khan University HospitalAga Khan University Hospital, Karachi Background: Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae have been reported previously from Pakistan but the genotypic characteristics of these enzymes is not known. Hence the aim of the study was first to characterise the genotypic content of these beta-lactamases and secondly to assess the clonal relationship of these isolates. Methodology: We analysed 65 non-duplicate ESBL positive, K. pneumoniae isolates prospectively collected based on phenotype as detected using the two-disc method. Isolates were collected from different sources: blood cultures (46.15%; n = 30); tracheal aspirates (24.6%; n = 16); urine (10.7%; n = 7); wound swabs, pus and tissue (18.4%; n = 12). ESBL production was confirmed by the ESBL E-test method and the presence of the blaCTX-M encoding genes was confirmed by polymerase chain reaction. The clonal relationship of clinical isolates was studied by Pulsed Field Gel Electrophoresis. Results: The results showed that 93.84% (n = 61) isolates of K. pneumoniae were positive for the blaCTX-M-1 group. One isolate showed PCR signals for blaCTX-M-25 group. None of our isolates were positive for CTX-M groups 2, 8 and 9. The majority of blaCTX-M positive isolates were genetically unrelated and no epidemic clones were identified. Conclusion: This study reports the emergence of CTX-M groups 1 and 25 producing isolates of K. pneumoniae with genetic diversity in Karachi, Pakistan. https://jidc.org/index.php/journal/article/view/674Gram negativeextended spectrum beta lactamasesmultidrug resistancehospital acquired infectionsnon-clonal isolatescarbapenam resistance. |
| spellingShingle | Erum Khan Thamarai Schneiders Afia Zafar Erum Aziz Asra Parekh Rumina Hasan Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan Journal of Infection in Developing Countries Gram negative extended spectrum beta lactamases multidrug resistance hospital acquired infections non-clonal isolates carbapenam resistance. |
| title | Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan |
| title_full | Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan |
| title_fullStr | Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan |
| title_full_unstemmed | Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan |
| title_short | Emergence of CTX-M Group 1-ESBL producing Klebsiella pneumonia from a tertiary care centre in Karachi, Pakistan |
| title_sort | emergence of ctx m group 1 esbl producing klebsiella pneumonia from a tertiary care centre in karachi pakistan |
| topic | Gram negative extended spectrum beta lactamases multidrug resistance hospital acquired infections non-clonal isolates carbapenam resistance. |
| url | https://jidc.org/index.php/journal/article/view/674 |
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