Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills
Objectives. The occurrence and development of nonalcoholic fatty liver disease (NAFLD) is related to lipid peroxidation, imbalance of inflammatory response factors, and immune function disorder. This study was conducted with the purpose of investigating the expression levels of inflammatory cytokine...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2022/8560831 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556684250185728 |
|---|---|
| author | Xiaohua Duan Jianlin Lv Hebei Jiang Kefei Zheng Yulin Chen |
| author_facet | Xiaohua Duan Jianlin Lv Hebei Jiang Kefei Zheng Yulin Chen |
| author_sort | Xiaohua Duan |
| collection | DOAJ |
| description | Objectives. The occurrence and development of nonalcoholic fatty liver disease (NAFLD) is related to lipid peroxidation, imbalance of inflammatory response factors, and immune function disorder. This study was conducted with the purpose of investigating the expression levels of inflammatory cytokines and adipocytokines and Th17/Treg balance in NAFLD patients treated with Dahuang Zhechong pills (DHZCPs). Methods. The study recruited 100 NAFLD patients who were then arranged into the test group and control group. Patients in the test group were treated with DHZCPs, while patients in the control group were untreated. Peripheral TH17 and Treg cells were detected by flow cytometry, and peripheral IL-17, IL-10, hs-CRP, and TNF-α expression levels were determined by enzyme-linked immunosorbent assay (ELISA) methods. The concentrations of ghrelin, leptin, and adiponectin were quantitatively examined. Results. The levels of TC, TG, ALT, and AST were declined but the level of HDL-C was increased in NAFLD patients treated with DHZCPs compared with untreated patients (P<0.05). The ratio of Th17/Treg in NAFLD patients treated with DHZCPs was (1.52 ± 0.21), which was significantly lower than (2.39 ± 0.45) of untreated patients (P<0.05). The levels of IL-17, hs-CRP, and TNF-α were lower, but the level of IL-10 was higher in NAFLD patients treated with DHZCPs than that in untreated patients (P<0.05). The expression levels of ghrelin and adiponectin in NAFLD patients treated with DHZCPs were evidently higher than those in untreated patients (P<0.01), and the expression level of leptin in NAFLD patients treated with DHZCPs was evidently lower than that in untreated patients (P<0.01). Conclusions. Administration of DHZCPs regulates the immune function of NAFLD patients by keeping Th17/Treg balance and affecting the levels of inflammatory cytokines and adipocytokines. |
| format | Article |
| id | doaj-art-811cfea04658414abcf72bfdc47c52de |
| institution | Kabale University |
| issn | 1469-5073 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj-art-811cfea04658414abcf72bfdc47c52de2025-02-03T05:44:38ZengWileyGenetics Research1469-50732022-01-01202210.1155/2022/8560831Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong PillsXiaohua Duan0Jianlin Lv1Hebei Jiang2Kefei Zheng3Yulin Chen4Medical Community of Yueqing Third People’s HospitalThe First Affiliated Hospital of Guangxi University of Chinese MedicineMedical Community of Yueqing Third People’s HospitalMedical Community of Yueqing Third People’s HospitalGuangxi School of Chinese MedicineObjectives. The occurrence and development of nonalcoholic fatty liver disease (NAFLD) is related to lipid peroxidation, imbalance of inflammatory response factors, and immune function disorder. This study was conducted with the purpose of investigating the expression levels of inflammatory cytokines and adipocytokines and Th17/Treg balance in NAFLD patients treated with Dahuang Zhechong pills (DHZCPs). Methods. The study recruited 100 NAFLD patients who were then arranged into the test group and control group. Patients in the test group were treated with DHZCPs, while patients in the control group were untreated. Peripheral TH17 and Treg cells were detected by flow cytometry, and peripheral IL-17, IL-10, hs-CRP, and TNF-α expression levels were determined by enzyme-linked immunosorbent assay (ELISA) methods. The concentrations of ghrelin, leptin, and adiponectin were quantitatively examined. Results. The levels of TC, TG, ALT, and AST were declined but the level of HDL-C was increased in NAFLD patients treated with DHZCPs compared with untreated patients (P<0.05). The ratio of Th17/Treg in NAFLD patients treated with DHZCPs was (1.52 ± 0.21), which was significantly lower than (2.39 ± 0.45) of untreated patients (P<0.05). The levels of IL-17, hs-CRP, and TNF-α were lower, but the level of IL-10 was higher in NAFLD patients treated with DHZCPs than that in untreated patients (P<0.05). The expression levels of ghrelin and adiponectin in NAFLD patients treated with DHZCPs were evidently higher than those in untreated patients (P<0.01), and the expression level of leptin in NAFLD patients treated with DHZCPs was evidently lower than that in untreated patients (P<0.01). Conclusions. Administration of DHZCPs regulates the immune function of NAFLD patients by keeping Th17/Treg balance and affecting the levels of inflammatory cytokines and adipocytokines.http://dx.doi.org/10.1155/2022/8560831 |
| spellingShingle | Xiaohua Duan Jianlin Lv Hebei Jiang Kefei Zheng Yulin Chen Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills Genetics Research |
| title | Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills |
| title_full | Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills |
| title_fullStr | Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills |
| title_full_unstemmed | Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills |
| title_short | Inflammatory Cytokines, Adipocytokines, and Th17/Treg Balance in Patients with Nonalcoholic Fatty Liver Disease following Administration of Dahuang Zhechong Pills |
| title_sort | inflammatory cytokines adipocytokines and th17 treg balance in patients with nonalcoholic fatty liver disease following administration of dahuang zhechong pills |
| url | http://dx.doi.org/10.1155/2022/8560831 |
| work_keys_str_mv | AT xiaohuaduan inflammatorycytokinesadipocytokinesandth17tregbalanceinpatientswithnonalcoholicfattyliverdiseasefollowingadministrationofdahuangzhechongpills AT jianlinlv inflammatorycytokinesadipocytokinesandth17tregbalanceinpatientswithnonalcoholicfattyliverdiseasefollowingadministrationofdahuangzhechongpills AT hebeijiang inflammatorycytokinesadipocytokinesandth17tregbalanceinpatientswithnonalcoholicfattyliverdiseasefollowingadministrationofdahuangzhechongpills AT kefeizheng inflammatorycytokinesadipocytokinesandth17tregbalanceinpatientswithnonalcoholicfattyliverdiseasefollowingadministrationofdahuangzhechongpills AT yulinchen inflammatorycytokinesadipocytokinesandth17tregbalanceinpatientswithnonalcoholicfattyliverdiseasefollowingadministrationofdahuangzhechongpills |