Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice
Summary: Here, we present a protocol for assessing the impact of a chemogenetic manipulation in a subpopulation of the hypothalamic neurons on aging and lifespan control using a mouse model developed specifically for this purpose. We describe steps for stereotaxic viral injection and assess inter-ti...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007160 |
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| author | Kyohei Tokizane Shin-ichiro Imai |
| author_facet | Kyohei Tokizane Shin-ichiro Imai |
| author_sort | Kyohei Tokizane |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for assessing the impact of a chemogenetic manipulation in a subpopulation of the hypothalamic neurons on aging and lifespan control using a mouse model developed specifically for this purpose. We describe steps for stereotaxic viral injection and assess inter-tissue communication between protein phosphatase 1 regulatory subunit 17 (Ppp1r17)-expressing neurons in the dorsomedial hypothalamus and white adipose tissue. We then detail procedures for lifespan measurements following chemogenetic manipulation in aged mice.For complete details on the use and execution of this protocol, please refer to Tokizane et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-81190567dddd48c798a23543609a6bfc |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-81190567dddd48c798a23543609a6bfc2025-01-11T06:41:59ZengElsevierSTAR Protocols2666-16672025-03-0161103551Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged miceKyohei Tokizane0Shin-ichiro Imai1Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USADepartment of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Corresponding authorSummary: Here, we present a protocol for assessing the impact of a chemogenetic manipulation in a subpopulation of the hypothalamic neurons on aging and lifespan control using a mouse model developed specifically for this purpose. We describe steps for stereotaxic viral injection and assess inter-tissue communication between protein phosphatase 1 regulatory subunit 17 (Ppp1r17)-expressing neurons in the dorsomedial hypothalamus and white adipose tissue. We then detail procedures for lifespan measurements following chemogenetic manipulation in aged mice.For complete details on the use and execution of this protocol, please refer to Tokizane et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007160MetabolismModel OrganismsNeuroscience |
| spellingShingle | Kyohei Tokizane Shin-ichiro Imai Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice STAR Protocols Metabolism Model Organisms Neuroscience |
| title | Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice |
| title_full | Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice |
| title_fullStr | Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice |
| title_full_unstemmed | Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice |
| title_short | Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice |
| title_sort | protocol to study inter tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice |
| topic | Metabolism Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007160 |
| work_keys_str_mv | AT kyoheitokizane protocoltostudyintertissuecommunicationbetweenthehypothalamusandwhiteadiposetissueandlifespanusingachemogeneticapproachinagedmice AT shinichiroimai protocoltostudyintertissuecommunicationbetweenthehypothalamusandwhiteadiposetissueandlifespanusingachemogeneticapproachinagedmice |