The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines
Purpose: to analyze the levels of cytokines in intraocular fluid (IF) and blood plasma of patients with diabetic macular edema (DME) previously untreated by intravitreal therapy before and after the therapy by angiogenesis inhibitor or a corticosteroid. Material and methods. We examined 90 people —...
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Real Time Ltd
2023-03-01
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| Series: | Российский офтальмологический журнал |
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| Online Access: | https://roj.igb.ru/jour/article/view/1180 |
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| author | A. Zh. Fursova A. S. Derbeneva O. S. Kozhevnikova D. V. Telegina V. A. Devyatkin |
| author_facet | A. Zh. Fursova A. S. Derbeneva O. S. Kozhevnikova D. V. Telegina V. A. Devyatkin |
| author_sort | A. Zh. Fursova |
| collection | DOAJ |
| description | Purpose: to analyze the levels of cytokines in intraocular fluid (IF) and blood plasma of patients with diabetic macular edema (DME) previously untreated by intravitreal therapy before and after the therapy by angiogenesis inhibitor or a corticosteroid. Material and methods. We examined 90 people — 47 females (52.2 %) and 43 males (47.8 %), mean age 64.54 ± 11.30 years. Of these, 60 had DME, and 30 formed the control group. The levels of 41 cytokines/chemokines in IF were determined by Milliplex® Map Human Cytokine/ Chemokine Panel; while the concentration of IL-18, MCP-1/CCL2, EPO, IL-10, IL-4, IL-6, IL-8, IFNα, VEGF-A in blood plasma was measured by enzyme-linked immunosorbent assay kits (Vector-Best, Russia). Patients with DME received intravitreal injections of an angiogenesis inhibitor (aflibercept. 50 eyes) or a corticosteroid (dexamethasone implant, 30 eyes). Results. Significant differences were revealed in 10 cytokine concentrations between the DME patients and the control group. The concentrations of IL-7, IL-15 and MCP-1/CCL2 levels in IF of DME patients were, respectively, 20.5, 20.3, and 11.02 times higher, than in the control group (р ˂ 0.05). Besides, a pairwise comparison of cytokines concentrations in IF of patients from either treatment group with the controls demonstrated a statistically significant increase in GROα/CXCL1 level. The pairwise comparison also revealed significant differences between the control and the corticosteroid therapy for systemic concentrations of IL-18 (p = 0.017), MCP-1/CCL2 (p = 0.009) and VEGF-A (p = 0.016). Conclusion. A pronounced and significant increase of the levels of a number of cytokines (e.g., IL-7, IL-15. FRACTALKINE/CX3CL1) were only sparsely reported before or remained undetermined at all. Our results on systemic cytokines levels may serve as prerequisite for further research into the role of systemic inflammation in DME pathogenesis. The analysis of associations of our results with those of other clinical biomarkers will contribute to the development of individualized treatment strategies. |
| format | Article |
| id | doaj-art-8113d3ccf40b42ec9b71f4d361267edc |
| institution | Kabale University |
| issn | 2072-0076 2587-5760 |
| language | Russian |
| publishDate | 2023-03-01 |
| publisher | Real Time Ltd |
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| series | Российский офтальмологический журнал |
| spelling | doaj-art-8113d3ccf40b42ec9b71f4d361267edc2025-08-20T03:39:28ZrusReal Time LtdРоссийский офтальмологический журнал2072-00762587-57602023-03-0116112713410.21516/2072-0076-2023-16-1-127-134560The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokinesA. Zh. Fursova0A. S. Derbeneva1O. S. Kozhevnikova2D. V. Telegina3V. A. Devyatkin4Novosibirsk State Regional Hospital; Novosibirsk State Medical UniversityNovosibirsk State Regional Hospital; Novosibirsk State Medical UniversityFederal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesFederal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesFederal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of SciencesPurpose: to analyze the levels of cytokines in intraocular fluid (IF) and blood plasma of patients with diabetic macular edema (DME) previously untreated by intravitreal therapy before and after the therapy by angiogenesis inhibitor or a corticosteroid. Material and methods. We examined 90 people — 47 females (52.2 %) and 43 males (47.8 %), mean age 64.54 ± 11.30 years. Of these, 60 had DME, and 30 formed the control group. The levels of 41 cytokines/chemokines in IF were determined by Milliplex® Map Human Cytokine/ Chemokine Panel; while the concentration of IL-18, MCP-1/CCL2, EPO, IL-10, IL-4, IL-6, IL-8, IFNα, VEGF-A in blood plasma was measured by enzyme-linked immunosorbent assay kits (Vector-Best, Russia). Patients with DME received intravitreal injections of an angiogenesis inhibitor (aflibercept. 50 eyes) or a corticosteroid (dexamethasone implant, 30 eyes). Results. Significant differences were revealed in 10 cytokine concentrations between the DME patients and the control group. The concentrations of IL-7, IL-15 and MCP-1/CCL2 levels in IF of DME patients were, respectively, 20.5, 20.3, and 11.02 times higher, than in the control group (р ˂ 0.05). Besides, a pairwise comparison of cytokines concentrations in IF of patients from either treatment group with the controls demonstrated a statistically significant increase in GROα/CXCL1 level. The pairwise comparison also revealed significant differences between the control and the corticosteroid therapy for systemic concentrations of IL-18 (p = 0.017), MCP-1/CCL2 (p = 0.009) and VEGF-A (p = 0.016). Conclusion. A pronounced and significant increase of the levels of a number of cytokines (e.g., IL-7, IL-15. FRACTALKINE/CX3CL1) were only sparsely reported before or remained undetermined at all. Our results on systemic cytokines levels may serve as prerequisite for further research into the role of systemic inflammation in DME pathogenesis. The analysis of associations of our results with those of other clinical biomarkers will contribute to the development of individualized treatment strategies.https://roj.igb.ru/jour/article/view/1180diabetic macular edemacytokinesintraocular fluidanti-angiogenic treatment |
| spellingShingle | A. Zh. Fursova A. S. Derbeneva O. S. Kozhevnikova D. V. Telegina V. A. Devyatkin The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines Российский офтальмологический журнал diabetic macular edema cytokines intraocular fluid anti-angiogenic treatment |
| title | The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines |
| title_full | The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines |
| title_fullStr | The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines |
| title_full_unstemmed | The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines |
| title_short | The impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines |
| title_sort | impact of intravitreal therapy of diabetic macular edema on the local and systemic production of cytokines |
| topic | diabetic macular edema cytokines intraocular fluid anti-angiogenic treatment |
| url | https://roj.igb.ru/jour/article/view/1180 |
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