Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst
Abstract Rising fuel security challenges can be addressed by utilizing potential and renewable energy feedstocks such as microalgae. This study focuses on biodiesel production from Desmodesmus armatus NCIM 5583 by optimizing biomass productivity and lipid content. Independent optimization experiment...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-04-01
|
| Series: | Discover Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s42452-025-06782-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145597693100032 |
|---|---|
| author | Supriya Pandey Ramesh Vinayagam Raja Selvaraj Thivaharan Varadavenkatesan |
| author_facet | Supriya Pandey Ramesh Vinayagam Raja Selvaraj Thivaharan Varadavenkatesan |
| author_sort | Supriya Pandey |
| collection | DOAJ |
| description | Abstract Rising fuel security challenges can be addressed by utilizing potential and renewable energy feedstocks such as microalgae. This study focuses on biodiesel production from Desmodesmus armatus NCIM 5583 by optimizing biomass productivity and lipid content. Independent optimization experiments were performed to study key growth parameters, viz., light intensity, photoperiod, and NaNO3 concentration. Growth conditions with 40 µmol/m2/s light intensity, 20 h L:04 h D photoperiod, and 0.25 g/L NaNO3 concentration exhibited highest biomass productivity of 26.51 ± 1.1 mg/L d and lipid yield of 45.1 ± 0.16% (w/w). Further, CaO bionanocatalyst synthesized using waste eggshells were characterized using SEM, EDX, FT-IR, and XRD. The results from SEM and EDX confirmed the structural and elemental composition of bionanocatalyst with Ca and O as the main elements. XRD revealed the crystalline nature of CaO with a particle size of 28.74 nm. Lipid transesterification using the synthesized catalyst yielded 84 ± 0.2% (w/w) FAME and was analyzed using FT-IR, 1H NMR, 13C NMR, and GC–MS. The results confirmed the presence of the methyl ester group, implying the conversion of microalgal lipids to biodiesel. The findings from this study demonstrate the potential of D. armatus for biodiesel production and the application of waste eggshells as transesterification catalysts. |
| format | Article |
| id | doaj-art-8110244357954a5d9892801f4e8d222e |
| institution | OA Journals |
| issn | 3004-9261 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Applied Sciences |
| spelling | doaj-art-8110244357954a5d9892801f4e8d222e2025-08-20T02:28:04ZengSpringerDiscover Applied Sciences3004-92612025-04-017411510.1007/s42452-025-06782-zOptimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalystSupriya Pandey0Ramesh Vinayagam1Raja Selvaraj2Thivaharan Varadavenkatesan3Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher EducationDepartment of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher EducationDepartment of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher EducationDepartment of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher EducationAbstract Rising fuel security challenges can be addressed by utilizing potential and renewable energy feedstocks such as microalgae. This study focuses on biodiesel production from Desmodesmus armatus NCIM 5583 by optimizing biomass productivity and lipid content. Independent optimization experiments were performed to study key growth parameters, viz., light intensity, photoperiod, and NaNO3 concentration. Growth conditions with 40 µmol/m2/s light intensity, 20 h L:04 h D photoperiod, and 0.25 g/L NaNO3 concentration exhibited highest biomass productivity of 26.51 ± 1.1 mg/L d and lipid yield of 45.1 ± 0.16% (w/w). Further, CaO bionanocatalyst synthesized using waste eggshells were characterized using SEM, EDX, FT-IR, and XRD. The results from SEM and EDX confirmed the structural and elemental composition of bionanocatalyst with Ca and O as the main elements. XRD revealed the crystalline nature of CaO with a particle size of 28.74 nm. Lipid transesterification using the synthesized catalyst yielded 84 ± 0.2% (w/w) FAME and was analyzed using FT-IR, 1H NMR, 13C NMR, and GC–MS. The results confirmed the presence of the methyl ester group, implying the conversion of microalgal lipids to biodiesel. The findings from this study demonstrate the potential of D. armatus for biodiesel production and the application of waste eggshells as transesterification catalysts.https://doi.org/10.1007/s42452-025-06782-zDesmodesmus armatusBiodieselLight intensityPhotoperiodNitrogen stressBionanocatalyst |
| spellingShingle | Supriya Pandey Ramesh Vinayagam Raja Selvaraj Thivaharan Varadavenkatesan Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst Discover Applied Sciences Desmodesmus armatus Biodiesel Light intensity Photoperiod Nitrogen stress Bionanocatalyst |
| title | Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst |
| title_full | Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst |
| title_fullStr | Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst |
| title_full_unstemmed | Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst |
| title_short | Optimizing growth conditions of Desmodesmus armatus NCIM 5583 for enhanced biodiesel production using CaO bionanocatalyst |
| title_sort | optimizing growth conditions of desmodesmus armatus ncim 5583 for enhanced biodiesel production using cao bionanocatalyst |
| topic | Desmodesmus armatus Biodiesel Light intensity Photoperiod Nitrogen stress Bionanocatalyst |
| url | https://doi.org/10.1007/s42452-025-06782-z |
| work_keys_str_mv | AT supriyapandey optimizinggrowthconditionsofdesmodesmusarmatusncim5583forenhancedbiodieselproductionusingcaobionanocatalyst AT rameshvinayagam optimizinggrowthconditionsofdesmodesmusarmatusncim5583forenhancedbiodieselproductionusingcaobionanocatalyst AT rajaselvaraj optimizinggrowthconditionsofdesmodesmusarmatusncim5583forenhancedbiodieselproductionusingcaobionanocatalyst AT thivaharanvaradavenkatesan optimizinggrowthconditionsofdesmodesmusarmatusncim5583forenhancedbiodieselproductionusingcaobionanocatalyst |