A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications

Ergothioneine (EGT), a natural thiol compound with potent antioxidant properties, exhibits diverse biological functions, including anti-inflammatory, neuroprotective, and cardioprotective effects. Despite its promising health and food applications, current production methods, such as mushroom-based...

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Main Authors: Haijing Zhang, Zheng Liu, Zhong Wang, Ziteng Lei, Yan Jia, Wei Chen, Ruoyu Shi, Chengtao Wang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/14/9/1588
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author Haijing Zhang
Zheng Liu
Zhong Wang
Ziteng Lei
Yan Jia
Wei Chen
Ruoyu Shi
Chengtao Wang
author_facet Haijing Zhang
Zheng Liu
Zhong Wang
Ziteng Lei
Yan Jia
Wei Chen
Ruoyu Shi
Chengtao Wang
author_sort Haijing Zhang
collection DOAJ
description Ergothioneine (EGT), a natural thiol compound with potent antioxidant properties, exhibits diverse biological functions, including anti-inflammatory, neuroprotective, and cardioprotective effects. Despite its promising health and food applications, current production methods, such as mushroom-based liquid fermentation, are hindered by low yields and complex processes. Advances in biosynthetic fermentation, including heterologous expression of key pathway genes and optimization of cultivation conditions, offer promising solutions to these challenges. Recent discoveries, such as the catalytic efficiency of mononuclear non-heme iron enzymes like Egt1 and EgtB, have streamlined EGT biosynthetic pathways, reducing steps and increasing yield. The compound’s active transport via the OCTN1 protein facilitates its distribution across tissues, enhancing its therapeutic efficacy and potential in functional foods. Currently employed as an antioxidant and antimelanogenic agent in aquatic products, EGT holds vast potential for broader applications in food systems. This review explores the advancements in EGT production and biosynthesis while emphasizing its prospects as a safe, versatile, and effective natural ingredient for health and industrial applications.
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institution Kabale University
issn 2304-8158
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj-art-8105889e2aef4e6896db6f7a38487c632025-08-20T03:52:58ZengMDPI AGFoods2304-81582025-04-01149158810.3390/foods14091588A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food ApplicationsHaijing Zhang0Zheng Liu1Zhong Wang2Ziteng Lei3Yan Jia4Wei Chen5Ruoyu Shi6Chengtao Wang7Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resources Research and Development, College of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaYunnan Plateau Characteristic Agricultural Industry Research Institute, Yunnan Agricultural University, Kunming 650201, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaErgothioneine (EGT), a natural thiol compound with potent antioxidant properties, exhibits diverse biological functions, including anti-inflammatory, neuroprotective, and cardioprotective effects. Despite its promising health and food applications, current production methods, such as mushroom-based liquid fermentation, are hindered by low yields and complex processes. Advances in biosynthetic fermentation, including heterologous expression of key pathway genes and optimization of cultivation conditions, offer promising solutions to these challenges. Recent discoveries, such as the catalytic efficiency of mononuclear non-heme iron enzymes like Egt1 and EgtB, have streamlined EGT biosynthetic pathways, reducing steps and increasing yield. The compound’s active transport via the OCTN1 protein facilitates its distribution across tissues, enhancing its therapeutic efficacy and potential in functional foods. Currently employed as an antioxidant and antimelanogenic agent in aquatic products, EGT holds vast potential for broader applications in food systems. This review explores the advancements in EGT production and biosynthesis while emphasizing its prospects as a safe, versatile, and effective natural ingredient for health and industrial applications.https://www.mdpi.com/2304-8158/14/9/1588ergothioneinebiosynthesisfood industryfermentationtherapeutic applicationsfunctional foods
spellingShingle Haijing Zhang
Zheng Liu
Zhong Wang
Ziteng Lei
Yan Jia
Wei Chen
Ruoyu Shi
Chengtao Wang
A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications
Foods
ergothioneine
biosynthesis
food industry
fermentation
therapeutic applications
functional foods
title A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications
title_full A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications
title_fullStr A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications
title_full_unstemmed A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications
title_short A Review of Novel Antioxidant Ergothioneine: Biosynthesis Pathways, Production, Function and Food Applications
title_sort review of novel antioxidant ergothioneine biosynthesis pathways production function and food applications
topic ergothioneine
biosynthesis
food industry
fermentation
therapeutic applications
functional foods
url https://www.mdpi.com/2304-8158/14/9/1588
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