Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells
Retinal degenerative diseases are one of the main causes of complete blindness in aged population. In this study, we compared the therapeutic potential for retinal degeneration of human mesenchymal stem cells derived from abdominal subcutaneous fat (ABASCs) or from orbital fat (OASCs) due to their a...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/7022247 |
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| author | Bryan Krief Shira Weisthal Algor Itay Nakdimon Ayala Elhikis Moshe Benhamou Anouk Savir Kadmon Shay Keren Oded Ohana Ilan Feldman Ran Ben Cnaan Igal Leibovitch Anat Loewenstein Adiel Barak Aya Barzelay |
| author_facet | Bryan Krief Shira Weisthal Algor Itay Nakdimon Ayala Elhikis Moshe Benhamou Anouk Savir Kadmon Shay Keren Oded Ohana Ilan Feldman Ran Ben Cnaan Igal Leibovitch Anat Loewenstein Adiel Barak Aya Barzelay |
| author_sort | Bryan Krief |
| collection | DOAJ |
| description | Retinal degenerative diseases are one of the main causes of complete blindness in aged population. In this study, we compared the therapeutic potential for retinal degeneration of human mesenchymal stem cells derived from abdominal subcutaneous fat (ABASCs) or from orbital fat (OASCs) due to their accessibility and mutual embryonic origin with retinal tissue, respectively. OASCs were found to protect RPE cells from cell death and were demonstrated to increase early RPE precursor markers, while ABASCs showed a raise in retinal precursor marker expression. Subretinal transplantation of OASCs in a mouse model of retinal degeneration led to restoration of the RPE layer while transplantation of ABASCs resulted in a significant restoration of the photoreceptor layer. Taken together, we demonstrated a lineage-specific therapeutic effect for either OASCs or ABASCs in retinal regeneration. |
| format | Article |
| id | doaj-art-8100a2a218d446878161e2a122c11d4e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8100a2a218d446878161e2a122c11d4e2025-02-03T07:23:30ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/70222477022247Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem CellsBryan Krief0Shira Weisthal Algor1Itay Nakdimon2Ayala Elhikis3Moshe Benhamou4Anouk Savir Kadmon5Shay Keren6Oded Ohana7Ilan Feldman8Ran Ben Cnaan9Igal Leibovitch10Anat Loewenstein11Adiel Barak12Aya Barzelay13Department of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelDepartment of Ophthalmology, Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, IsraelRetinal degenerative diseases are one of the main causes of complete blindness in aged population. In this study, we compared the therapeutic potential for retinal degeneration of human mesenchymal stem cells derived from abdominal subcutaneous fat (ABASCs) or from orbital fat (OASCs) due to their accessibility and mutual embryonic origin with retinal tissue, respectively. OASCs were found to protect RPE cells from cell death and were demonstrated to increase early RPE precursor markers, while ABASCs showed a raise in retinal precursor marker expression. Subretinal transplantation of OASCs in a mouse model of retinal degeneration led to restoration of the RPE layer while transplantation of ABASCs resulted in a significant restoration of the photoreceptor layer. Taken together, we demonstrated a lineage-specific therapeutic effect for either OASCs or ABASCs in retinal regeneration.http://dx.doi.org/10.1155/2021/7022247 |
| spellingShingle | Bryan Krief Shira Weisthal Algor Itay Nakdimon Ayala Elhikis Moshe Benhamou Anouk Savir Kadmon Shay Keren Oded Ohana Ilan Feldman Ran Ben Cnaan Igal Leibovitch Anat Loewenstein Adiel Barak Aya Barzelay Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells Stem Cells International |
| title | Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells |
| title_full | Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells |
| title_fullStr | Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells |
| title_short | Retinal Lineage Therapeutic Specific Effect of Human Orbital and Abdominal Adipose-Derived Mesenchymal Stem Cells |
| title_sort | retinal lineage therapeutic specific effect of human orbital and abdominal adipose derived mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2021/7022247 |
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