Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration
Hematopoietic stem cells derived from bone marrow are known to induce tissue repair. Their mechanisms to induce liver regeneration are not clear and may have numerous adverse effects. We compared the regenerative potential of intact hematopoietic stem cells (iHSCs), their total RNA, and apoptotic he...
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2025-03-01
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| author | Nina Onishchenko Murat Shagidulin Zalina Gonikova Alla Nikolskaya Ludmila Kirsanova Artem Venediktov Ksenia Pokidova Egor Kuzmin Natalia Kuznetsova Igor Kozlov Dmitry Telyshev Natalia Kartashkina Viacheslav Varentsov Maria Timofeeva Victor Sevastjanov Andrei Elchaninov Gennadii Piavchenko Sergey Gautier |
| author_facet | Nina Onishchenko Murat Shagidulin Zalina Gonikova Alla Nikolskaya Ludmila Kirsanova Artem Venediktov Ksenia Pokidova Egor Kuzmin Natalia Kuznetsova Igor Kozlov Dmitry Telyshev Natalia Kartashkina Viacheslav Varentsov Maria Timofeeva Victor Sevastjanov Andrei Elchaninov Gennadii Piavchenko Sergey Gautier |
| author_sort | Nina Onishchenko |
| collection | DOAJ |
| description | Hematopoietic stem cells derived from bone marrow are known to induce tissue repair. Their mechanisms to induce liver regeneration are not clear and may have numerous adverse effects. We compared the regenerative potential of intact hematopoietic stem cells (iHSCs), their total RNA, and apoptotic hematopoietic stem cells (aHSCs) in liver damage. Male Wistar rats (<i>n</i> = 40 per group) experienced a 75% liver resection and received single intraperitoneal injections of saline (control group), iHSCs, aHSCs, or total RNA of iHSCs. We recorded animal survival, liver mass, blood markers of liver cell lysis and function (albumin, aminotransferases, alkaline phosphatase), and liver histological features: mitotic index and expression of markers for proliferation and apoptosis (caspase-9, caspase-3, and Ki-67). We assessed the survival with the log-rank test and used Wilcoxon and t tests for the other parameters. Animals of all HSC- or RNA-treated groups survived until the end of the experiment with full blood marker recovery (<i>p</i> = 0.037). The liver mass enlarged mostly after apoptotic hematopoietic stem cells and total RNA. Mitotic index peaked in the group of total RNA with a lower but earlier increase for the aHSC group, and Ki-67 proliferation for the total RNA group was the highest (all the differences were significant with <i>p</i> values ˂ 0.05). We found the aHSCs and total RNA of iHSC group to be the most efficient for liver reparation. Total RNA from HSCs is preferred for further liver regeneration studies. |
| format | Article |
| id | doaj-art-80eeab47c4684961a9164f5a848b8b00 |
| institution | DOAJ |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Applied Sciences |
| spelling | doaj-art-80eeab47c4684961a9164f5a848b8b002025-08-20T03:06:32ZengMDPI AGApplied Sciences2076-34172025-03-01157378210.3390/app15073782Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver RegenerationNina Onishchenko0Murat Shagidulin1Zalina Gonikova2Alla Nikolskaya3Ludmila Kirsanova4Artem Venediktov5Ksenia Pokidova6Egor Kuzmin7Natalia Kuznetsova8Igor Kozlov9Dmitry Telyshev10Natalia Kartashkina11Viacheslav Varentsov12Maria Timofeeva13Victor Sevastjanov14Andrei Elchaninov15Gennadii Piavchenko16Sergey Gautier17Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaKulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, 117198 Moscow, RussiaDepartment of Human Anatomy and Histology, I. M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaShumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaHematopoietic stem cells derived from bone marrow are known to induce tissue repair. Their mechanisms to induce liver regeneration are not clear and may have numerous adverse effects. We compared the regenerative potential of intact hematopoietic stem cells (iHSCs), their total RNA, and apoptotic hematopoietic stem cells (aHSCs) in liver damage. Male Wistar rats (<i>n</i> = 40 per group) experienced a 75% liver resection and received single intraperitoneal injections of saline (control group), iHSCs, aHSCs, or total RNA of iHSCs. We recorded animal survival, liver mass, blood markers of liver cell lysis and function (albumin, aminotransferases, alkaline phosphatase), and liver histological features: mitotic index and expression of markers for proliferation and apoptosis (caspase-9, caspase-3, and Ki-67). We assessed the survival with the log-rank test and used Wilcoxon and t tests for the other parameters. Animals of all HSC- or RNA-treated groups survived until the end of the experiment with full blood marker recovery (<i>p</i> = 0.037). The liver mass enlarged mostly after apoptotic hematopoietic stem cells and total RNA. Mitotic index peaked in the group of total RNA with a lower but earlier increase for the aHSC group, and Ki-67 proliferation for the total RNA group was the highest (all the differences were significant with <i>p</i> values ˂ 0.05). We found the aHSCs and total RNA of iHSC group to be the most efficient for liver reparation. Total RNA from HSCs is preferred for further liver regeneration studies.https://www.mdpi.com/2076-3417/15/7/3782liver resectionapoptotic stem cellsintact stem cellscell graftingregenerative signalingRNA-induced regeneration |
| spellingShingle | Nina Onishchenko Murat Shagidulin Zalina Gonikova Alla Nikolskaya Ludmila Kirsanova Artem Venediktov Ksenia Pokidova Egor Kuzmin Natalia Kuznetsova Igor Kozlov Dmitry Telyshev Natalia Kartashkina Viacheslav Varentsov Maria Timofeeva Victor Sevastjanov Andrei Elchaninov Gennadii Piavchenko Sergey Gautier Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration Applied Sciences liver resection apoptotic stem cells intact stem cells cell grafting regenerative signaling RNA-induced regeneration |
| title | Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration |
| title_full | Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration |
| title_fullStr | Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration |
| title_full_unstemmed | Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration |
| title_short | Hematopoietic Stem Cells of Bone Marrow and Their Total RNA in Rat Liver Regeneration |
| title_sort | hematopoietic stem cells of bone marrow and their total rna in rat liver regeneration |
| topic | liver resection apoptotic stem cells intact stem cells cell grafting regenerative signaling RNA-induced regeneration |
| url | https://www.mdpi.com/2076-3417/15/7/3782 |
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