Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.

Disrupted circadian rhythms and reduced sleep duration are associated with several human diseases, particularly obesity and type 2 diabetes, but until recently, little was known about the genetic factors influencing these heritable traits. We performed genome-wide association studies of self-reporte...

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Main Authors: Samuel E Jones, Jessica Tyrrell, Andrew R Wood, Robin N Beaumont, Katherine S Ruth, Marcus A Tuke, Hanieh Yaghootkar, Youna Hu, Maris Teder-Laving, Caroline Hayward, Till Roenneberg, James F Wilson, Fabiola Del Greco, Andrew A Hicks, Chol Shin, Chang-Ho Yun, Seung Ku Lee, Andres Metspalu, Enda M Byrne, Philip R Gehrman, Henning Tiemeier, Karla V Allebrandt, Rachel M Freathy, Anna Murray, David A Hinds, Timothy M Frayling, Michael N Weedon
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-08-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006125&type=printable
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author Samuel E Jones
Jessica Tyrrell
Andrew R Wood
Robin N Beaumont
Katherine S Ruth
Marcus A Tuke
Hanieh Yaghootkar
Youna Hu
Maris Teder-Laving
Caroline Hayward
Till Roenneberg
James F Wilson
Fabiola Del Greco
Andrew A Hicks
Chol Shin
Chang-Ho Yun
Seung Ku Lee
Andres Metspalu
Enda M Byrne
Philip R Gehrman
Henning Tiemeier
Karla V Allebrandt
Rachel M Freathy
Anna Murray
David A Hinds
Timothy M Frayling
Michael N Weedon
author_facet Samuel E Jones
Jessica Tyrrell
Andrew R Wood
Robin N Beaumont
Katherine S Ruth
Marcus A Tuke
Hanieh Yaghootkar
Youna Hu
Maris Teder-Laving
Caroline Hayward
Till Roenneberg
James F Wilson
Fabiola Del Greco
Andrew A Hicks
Chol Shin
Chang-Ho Yun
Seung Ku Lee
Andres Metspalu
Enda M Byrne
Philip R Gehrman
Henning Tiemeier
Karla V Allebrandt
Rachel M Freathy
Anna Murray
David A Hinds
Timothy M Frayling
Michael N Weedon
author_sort Samuel E Jones
collection DOAJ
description Disrupted circadian rhythms and reduced sleep duration are associated with several human diseases, particularly obesity and type 2 diabetes, but until recently, little was known about the genetic factors influencing these heritable traits. We performed genome-wide association studies of self-reported chronotype (morning/evening person) and self-reported sleep duration in 128,266 white British individuals from the UK Biobank study. Sixteen variants were associated with chronotype (P<5x10-8), including variants near the known circadian rhythm genes RGS16 (1.21 odds of morningness, 95% CI [1.15, 1.27], P = 3x10-12) and PER2 (1.09 odds of morningness, 95% CI [1.06, 1.12], P = 4x10-10). The PER2 signal has previously been associated with iris function. We sought replication using self-reported data from 89,283 23andMe participants; thirteen of the chronotype signals remained associated at P<5x10-8 on meta-analysis and eleven of these reached P<0.05 in the same direction in the 23andMe study. We also replicated 9 additional variants identified when the 23andMe study was used as a discovery GWAS of chronotype (all P<0.05 and meta-analysis P<5x10-8). For sleep duration, we replicated one known signal in PAX8 (2.6 minutes per allele, 95% CI [1.9, 3.2], P = 5.7x10-16) and identified and replicated two novel associations at VRK2 (2.0 minutes per allele, 95% CI [1.3, 2.7], P = 1.2x10-9; and 1.6 minutes per allele, 95% CI [1.1, 2.2], P = 7.6x10-9). Although we found genetic correlation between chronotype and BMI (rG = 0.056, P = 0.05); undersleeping and BMI (rG = 0.147, P = 1x10-5) and oversleeping and BMI (rG = 0.097, P = 0.04), Mendelian Randomisation analyses, with limited power, provided no consistent evidence of causal associations between BMI or type 2 diabetes and chronotype or sleep duration. Our study brings the total number of loci associated with chronotype to 22 and with sleep duration to three, and provides new insights into the biology of sleep and circadian rhythms in humans.
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spelling doaj-art-80eac3f9df0149afabc3a13a112d8fb92025-08-20T03:26:09ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-08-01128e100612510.1371/journal.pgen.1006125Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.Samuel E JonesJessica TyrrellAndrew R WoodRobin N BeaumontKatherine S RuthMarcus A TukeHanieh YaghootkarYouna HuMaris Teder-LavingCaroline HaywardTill RoennebergJames F WilsonFabiola Del GrecoAndrew A HicksChol ShinChang-Ho YunSeung Ku LeeAndres MetspaluEnda M ByrnePhilip R GehrmanHenning TiemeierKarla V AllebrandtRachel M FreathyAnna MurrayDavid A HindsTimothy M FraylingMichael N WeedonDisrupted circadian rhythms and reduced sleep duration are associated with several human diseases, particularly obesity and type 2 diabetes, but until recently, little was known about the genetic factors influencing these heritable traits. We performed genome-wide association studies of self-reported chronotype (morning/evening person) and self-reported sleep duration in 128,266 white British individuals from the UK Biobank study. Sixteen variants were associated with chronotype (P<5x10-8), including variants near the known circadian rhythm genes RGS16 (1.21 odds of morningness, 95% CI [1.15, 1.27], P = 3x10-12) and PER2 (1.09 odds of morningness, 95% CI [1.06, 1.12], P = 4x10-10). The PER2 signal has previously been associated with iris function. We sought replication using self-reported data from 89,283 23andMe participants; thirteen of the chronotype signals remained associated at P<5x10-8 on meta-analysis and eleven of these reached P<0.05 in the same direction in the 23andMe study. We also replicated 9 additional variants identified when the 23andMe study was used as a discovery GWAS of chronotype (all P<0.05 and meta-analysis P<5x10-8). For sleep duration, we replicated one known signal in PAX8 (2.6 minutes per allele, 95% CI [1.9, 3.2], P = 5.7x10-16) and identified and replicated two novel associations at VRK2 (2.0 minutes per allele, 95% CI [1.3, 2.7], P = 1.2x10-9; and 1.6 minutes per allele, 95% CI [1.1, 2.2], P = 7.6x10-9). Although we found genetic correlation between chronotype and BMI (rG = 0.056, P = 0.05); undersleeping and BMI (rG = 0.147, P = 1x10-5) and oversleeping and BMI (rG = 0.097, P = 0.04), Mendelian Randomisation analyses, with limited power, provided no consistent evidence of causal associations between BMI or type 2 diabetes and chronotype or sleep duration. Our study brings the total number of loci associated with chronotype to 22 and with sleep duration to three, and provides new insights into the biology of sleep and circadian rhythms in humans.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006125&type=printable
spellingShingle Samuel E Jones
Jessica Tyrrell
Andrew R Wood
Robin N Beaumont
Katherine S Ruth
Marcus A Tuke
Hanieh Yaghootkar
Youna Hu
Maris Teder-Laving
Caroline Hayward
Till Roenneberg
James F Wilson
Fabiola Del Greco
Andrew A Hicks
Chol Shin
Chang-Ho Yun
Seung Ku Lee
Andres Metspalu
Enda M Byrne
Philip R Gehrman
Henning Tiemeier
Karla V Allebrandt
Rachel M Freathy
Anna Murray
David A Hinds
Timothy M Frayling
Michael N Weedon
Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.
PLoS Genetics
title Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.
title_full Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.
title_fullStr Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.
title_full_unstemmed Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.
title_short Genome-Wide Association Analyses in 128,266 Individuals Identifies New Morningness and Sleep Duration Loci.
title_sort genome wide association analyses in 128 266 individuals identifies new morningness and sleep duration loci
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006125&type=printable
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