The telomere-to-telomere genome assembly and annotation of the rock carp (Procypris rabaudi)

Abstract Procypris rabaudi, commonly known as the rock carp, is an endemic economic fish in the middle-upper reaches of the Yangtze River. To enhance the understanding the biology of rack carps, a high-quality reference genome is required in different areas of study. Here, we generated the first tel...

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Bibliographic Details
Main Authors: Xiaolu Han, Xinle Li, Luohao Xu, Qi Liu, Shengqi Su, Weizhi Yao, Wenping He
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05066-w
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Summary:Abstract Procypris rabaudi, commonly known as the rock carp, is an endemic economic fish in the middle-upper reaches of the Yangtze River. To enhance the understanding the biology of rack carps, a high-quality reference genome is required in different areas of study. Here, we generated the first telomere-to-telomere genome assembly and annotation of the rock carp, which spans 1.64 Gb with a contig N50 of 32.36 Mb. Hi-C assembly suggested that 99.83% sequences were positioned to 50 pseudo-chromosomes. Notably, 43 chromosomes were assembled without any gap. Through the integration of homologous-based predictions and RNA-sequencing technology, we identified 44,402 protein-coding genes, with 43,663 of them (98.3%) predicted to be functional. Furthermore, our assembled genome achieved 98.1% BUSCO completeness. This work improves the quality of the rock carp genome and provides valuable foundation for the future studies of genomics, biology and the fishery resources breeding.
ISSN:2052-4463