SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE
The aim is to conduct the biodiagnosis of soil sampled in the area of the underground storage of natural gas (UGS) (Stepnoye village, Saratov region) with the use of microbiological analysis.Methods. In the course of the work, using Koch’s solid medium method were estimated the following: the total...
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Kamerton
2017-07-01
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| Series: | Юг России: экология, развитие |
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| Online Access: | https://ecodag.elpub.ru/ugro/article/view/933 |
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| author | E. V. Pleshakova C. T. Ngun M. V. Reshetnikov |
| author_facet | E. V. Pleshakova C. T. Ngun M. V. Reshetnikov |
| author_sort | E. V. Pleshakova |
| collection | DOAJ |
| description | The aim is to conduct the biodiagnosis of soil sampled in the area of the underground storage of natural gas (UGS) (Stepnoye village, Saratov region) with the use of microbiological analysis.Methods. In the course of the work, using Koch’s solid medium method were estimated the following: the total number of heterotrophic microorganisms on meat peptone agar, amount of methylotrophic, hydrocarbon oxidizing, ironoxidizing and sulfur-oxidizing bacteria in the respective selective mediums; as well as the hydrogen index, oxidation-reduction potential and magnetic susceptibility of the soil.Results. Microbiological analysis of the soil in the Stepnovsky UGS area has shown changes in soil biocenoses. A reduced content of heterotrophic microorganisms was detected in comparison with background samples. In a number of samples, we also discovered an increased content of hydrocarbon-oxidizing and methylotrophic microorganisms, including obligate ones, which suggest the methane entering the upper layers of the soil. An increased content of sulfur-oxidizing bacteria was also detected; the presence of iron-oxidizing microorganisms in the soil was shown.Conclusions. The revealed features in the content of indicator microorganisms in the soil sampled in the area of the underground storage of natural gas, indicating the development of specialized soil microorganisms, confirmed the presence of contaminants in the soil which are hydrocarbons and sulfur compounds. The use of microbiological indicator values seems promising for environmental monitoring of soils in the areas of gas storage and early detection of methane leakage. |
| format | Article |
| id | doaj-art-80e629a126f2437a8d82ccc1513aefd2 |
| institution | DOAJ |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| publishDate | 2017-07-01 |
| publisher | Kamerton |
| record_format | Article |
| series | Юг России: экология, развитие |
| spelling | doaj-art-80e629a126f2437a8d82ccc1513aefd22025-08-20T03:00:07ZrusKamertonЮг России: экология, развитие1992-10982413-09582017-07-0112213514610.18470/1992-1098-2017-2-135-146908SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGEE. V. Pleshakova0C. T. Ngun1M. V. Reshetnikov2National Research Saratov State University named after N.G. ChernyshevskyNational Research Saratov State University named after N.G. ChernyshevskyNational Research Saratov State University named after N.G. ChernyshevskyThe aim is to conduct the biodiagnosis of soil sampled in the area of the underground storage of natural gas (UGS) (Stepnoye village, Saratov region) with the use of microbiological analysis.Methods. In the course of the work, using Koch’s solid medium method were estimated the following: the total number of heterotrophic microorganisms on meat peptone agar, amount of methylotrophic, hydrocarbon oxidizing, ironoxidizing and sulfur-oxidizing bacteria in the respective selective mediums; as well as the hydrogen index, oxidation-reduction potential and magnetic susceptibility of the soil.Results. Microbiological analysis of the soil in the Stepnovsky UGS area has shown changes in soil biocenoses. A reduced content of heterotrophic microorganisms was detected in comparison with background samples. In a number of samples, we also discovered an increased content of hydrocarbon-oxidizing and methylotrophic microorganisms, including obligate ones, which suggest the methane entering the upper layers of the soil. An increased content of sulfur-oxidizing bacteria was also detected; the presence of iron-oxidizing microorganisms in the soil was shown.Conclusions. The revealed features in the content of indicator microorganisms in the soil sampled in the area of the underground storage of natural gas, indicating the development of specialized soil microorganisms, confirmed the presence of contaminants in the soil which are hydrocarbons and sulfur compounds. The use of microbiological indicator values seems promising for environmental monitoring of soils in the areas of gas storage and early detection of methane leakage.https://ecodag.elpub.ru/ugro/article/view/933underground storage of natural gasheterotrophicmethylotrophichydrocarbon oxidizingiron oxidizingsulfur oxidizing microorganisms |
| spellingShingle | E. V. Pleshakova C. T. Ngun M. V. Reshetnikov SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE Юг России: экология, развитие underground storage of natural gas heterotrophic methylotrophic hydrocarbon oxidizing iron oxidizing sulfur oxidizing microorganisms |
| title | SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE |
| title_full | SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE |
| title_fullStr | SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE |
| title_full_unstemmed | SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE |
| title_short | SOIL ANALYSIS ON MICROBIOLOGICAL INDICATOR VALUES IN THE AREA OF UNDERGROUND NATURE GAS STORAGE |
| title_sort | soil analysis on microbiological indicator values in the area of underground nature gas storage |
| topic | underground storage of natural gas heterotrophic methylotrophic hydrocarbon oxidizing iron oxidizing sulfur oxidizing microorganisms |
| url | https://ecodag.elpub.ru/ugro/article/view/933 |
| work_keys_str_mv | AT evpleshakova soilanalysisonmicrobiologicalindicatorvaluesintheareaofundergroundnaturegasstorage AT ctngun soilanalysisonmicrobiologicalindicatorvaluesintheareaofundergroundnaturegasstorage AT mvreshetnikov soilanalysisonmicrobiologicalindicatorvaluesintheareaofundergroundnaturegasstorage |