Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer
Epstein–Barr virus (EBV)‐associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12765 |
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| author | Hirofumi Ohmura Mamoru Ito Keita Uchino Chihiro Okada Shigeki Tanishima Yuichi Yamada Seiya Momosaki Masato Komoda Miyuki Kuwayama Kyoko Yamaguchi Yuta Okumura Michitaka Nakano Kenji Tsuchihashi Taichi Isobe Hiroshi Ariyama Hitoshi Kusaba Yoshinao Oda Koichi Akashi Eishi Baba |
| author_facet | Hirofumi Ohmura Mamoru Ito Keita Uchino Chihiro Okada Shigeki Tanishima Yuichi Yamada Seiya Momosaki Masato Komoda Miyuki Kuwayama Kyoko Yamaguchi Yuta Okumura Michitaka Nakano Kenji Tsuchihashi Taichi Isobe Hiroshi Ariyama Hitoshi Kusaba Yoshinao Oda Koichi Akashi Eishi Baba |
| author_sort | Hirofumi Ohmura |
| collection | DOAJ |
| description | Epstein–Barr virus (EBV)‐associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from an advanced EBV‐associated GC (EBVGC) case, in which complete response to S‐1 plus cisplatin chemotherapy was achieved, was analyzed using a DNA methylation microarray. We compared DNA methylation of GC cells with public data and identified genes with higher methylation in EBVGC cell lines than in normal gastric cells, and genes in which methylation was increased by EBV. Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5‐fluorouracil and cisplatin. Silencing of these genes may be associated with hypersensitivity to chemotherapy. |
| format | Article |
| id | doaj-art-80d7b84ba5f14786b0a6e4b701c596bc |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-80d7b84ba5f14786b0a6e4b701c596bc2025-08-20T02:34:25ZengWileyFEBS Open Bio2211-54632020-01-0110114715710.1002/2211-5463.12765Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancerHirofumi Ohmura0Mamoru Ito1Keita Uchino2Chihiro Okada3Shigeki Tanishima4Yuichi Yamada5Seiya Momosaki6Masato Komoda7Miyuki Kuwayama8Kyoko Yamaguchi9Yuta Okumura10Michitaka Nakano11Kenji Tsuchihashi12Taichi Isobe13Hiroshi Ariyama14Hitoshi Kusaba15Yoshinao Oda16Koichi Akashi17Eishi Baba18Department of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Clinical Oncology NTT Medical Center Tokyo JapanEngineering Section Biomedical Informatics Development Department Kansai Division Mitsubishi Space Software Hyogo JapanEngineering Section Biomedical Informatics Development Department Kansai Division Mitsubishi Space Software Hyogo JapanDepartment of Anatomic Pathology Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Pathology National Hospital Organization Kyushu Medical Center Fukuoka JapanDepartment of Gastrointestinal and Medical Oncology National Hospital Organization Kyushu Cancer Center Fukuoka JapanDepartment of Internal Medicine Munakata Medical Association Hospital Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Internal Medicine Kyushu University Beppu Hospital Oita JapanDepartment of Gastrointestinal and Medical Oncology National Hospital Organization Kyushu Cancer Center Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Anatomic Pathology Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences Fukuoka JapanDepartment of Oncology and Social Medicine Kyushu University Graduate School of Medical Sciences Fukuoka JapanEpstein–Barr virus (EBV)‐associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from an advanced EBV‐associated GC (EBVGC) case, in which complete response to S‐1 plus cisplatin chemotherapy was achieved, was analyzed using a DNA methylation microarray. We compared DNA methylation of GC cells with public data and identified genes with higher methylation in EBVGC cell lines than in normal gastric cells, and genes in which methylation was increased by EBV. Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5‐fluorouracil and cisplatin. Silencing of these genes may be associated with hypersensitivity to chemotherapy.https://doi.org/10.1002/2211-5463.12765chemotherapyDNA methylationdrug susceptibilityEBVEpstein‐Barr virus‐associated gastric cancergastric cancer |
| spellingShingle | Hirofumi Ohmura Mamoru Ito Keita Uchino Chihiro Okada Shigeki Tanishima Yuichi Yamada Seiya Momosaki Masato Komoda Miyuki Kuwayama Kyoko Yamaguchi Yuta Okumura Michitaka Nakano Kenji Tsuchihashi Taichi Isobe Hiroshi Ariyama Hitoshi Kusaba Yoshinao Oda Koichi Akashi Eishi Baba Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer FEBS Open Bio chemotherapy DNA methylation drug susceptibility EBV Epstein‐Barr virus‐associated gastric cancer gastric cancer |
| title | Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer |
| title_full | Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer |
| title_fullStr | Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer |
| title_full_unstemmed | Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer |
| title_short | Methylation of drug resistance‐related genes in chemotherapy‐sensitive Epstein–Barr virus‐associated gastric cancer |
| title_sort | methylation of drug resistance related genes in chemotherapy sensitive epstein barr virus associated gastric cancer |
| topic | chemotherapy DNA methylation drug susceptibility EBV Epstein‐Barr virus‐associated gastric cancer gastric cancer |
| url | https://doi.org/10.1002/2211-5463.12765 |
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