Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas.
Previous studies have examined the cellulase activity of Crassostrea gigas (Pacific oyster) and suggested its potential utilization of terrestrial lignocellulose. However, no studies have been conducted to comprehensively assess its endogenous cellulases. Therefore, our objective was to identify the...
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0313246 |
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| author | Manabu Wen-Liu Tanimura Kazuhiko Koike Motoki Kayama Kazumi Matsuoka |
| author_facet | Manabu Wen-Liu Tanimura Kazuhiko Koike Motoki Kayama Kazumi Matsuoka |
| author_sort | Manabu Wen-Liu Tanimura |
| collection | DOAJ |
| description | Previous studies have examined the cellulase activity of Crassostrea gigas (Pacific oyster) and suggested its potential utilization of terrestrial lignocellulose. However, no studies have been conducted to comprehensively assess its endogenous cellulases. Therefore, our objective was to identify the cellulases present in C. gigas through transcriptome and genomic analyses. The results showed that there are 10 cellulase orthologs, seven of which are endogenous. Phylogenetic analysis revealed that two of these cellulases belong to the glycoside hydrolase family (GHF) 5, four to GHF9, and one to GHF45. An alignment of the amino acid sequences suggested the presence of at least endo-β-1,4-glucanase. Therefore, C. gigas is likely capable of decomposing lignocellulose into glucose. This finding supports the fact that C. gigas, a globally commercial bivalve species, thrives in environments that lack phytoplankton, such as mangroves. |
| format | Article |
| id | doaj-art-80c0c8998ebb44d792e03c9c7d4c377e |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-80c0c8998ebb44d792e03c9c7d4c377e2025-02-12T05:31:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031324610.1371/journal.pone.0313246Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas.Manabu Wen-Liu TanimuraKazuhiko KoikeMotoki KayamaKazumi MatsuokaPrevious studies have examined the cellulase activity of Crassostrea gigas (Pacific oyster) and suggested its potential utilization of terrestrial lignocellulose. However, no studies have been conducted to comprehensively assess its endogenous cellulases. Therefore, our objective was to identify the cellulases present in C. gigas through transcriptome and genomic analyses. The results showed that there are 10 cellulase orthologs, seven of which are endogenous. Phylogenetic analysis revealed that two of these cellulases belong to the glycoside hydrolase family (GHF) 5, four to GHF9, and one to GHF45. An alignment of the amino acid sequences suggested the presence of at least endo-β-1,4-glucanase. Therefore, C. gigas is likely capable of decomposing lignocellulose into glucose. This finding supports the fact that C. gigas, a globally commercial bivalve species, thrives in environments that lack phytoplankton, such as mangroves.https://doi.org/10.1371/journal.pone.0313246 |
| spellingShingle | Manabu Wen-Liu Tanimura Kazuhiko Koike Motoki Kayama Kazumi Matsuoka Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas. PLoS ONE |
| title | Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas. |
| title_full | Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas. |
| title_fullStr | Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas. |
| title_full_unstemmed | Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas. |
| title_short | Comprehensive cDNA cloning and putative feature analysis of endogenous cellulases possessed by the Pacific oyster, Crassostrea gigas. |
| title_sort | comprehensive cdna cloning and putative feature analysis of endogenous cellulases possessed by the pacific oyster crassostrea gigas |
| url | https://doi.org/10.1371/journal.pone.0313246 |
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