Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer
Background. Buruli ulcer (BU) continues to be a serious public health threat in wet tropical regions and the mode of transmission of its etiological agent, Mycobacterium ulcerans (MU), remains poorly understood. In this study, mosquito species collected in endemic villages in Benin were screened for...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/1324310 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562884944592896 |
|---|---|
| author | Rousseau Djouaka Francis Zeukeng Jude Daiga Bigoga David N’golo Coulibaly Genevieve Tchigossou Romaric Akoton Sylla Aboubacar Sodjinin Jean-Eudes Tchebe Clavella Nantcho Nguepdjo Razack Adeoti Innocent Djegbe Manuele Tamo Wilfred Fon Mbacham Solange E. Kakou-Ngazoa Anthony Ablordey |
| author_facet | Rousseau Djouaka Francis Zeukeng Jude Daiga Bigoga David N’golo Coulibaly Genevieve Tchigossou Romaric Akoton Sylla Aboubacar Sodjinin Jean-Eudes Tchebe Clavella Nantcho Nguepdjo Razack Adeoti Innocent Djegbe Manuele Tamo Wilfred Fon Mbacham Solange E. Kakou-Ngazoa Anthony Ablordey |
| author_sort | Rousseau Djouaka |
| collection | DOAJ |
| description | Background. Buruli ulcer (BU) continues to be a serious public health threat in wet tropical regions and the mode of transmission of its etiological agent, Mycobacterium ulcerans (MU), remains poorly understood. In this study, mosquito species collected in endemic villages in Benin were screened for the presence of MU. In addition, the ability of mosquitoes larvae to pick up MU from their environment and remain colonized through the larval developmental stages to the adult stage was investigated. Methods. 7,218 adults and larvae mosquitoes were sampled from endemic and nonendemic villages and screened for MU DNA targets (IS2404, IS2606, and KR-B) using qPCR. Results. MU was not detected in any of the field collected samples. Additional studies of artificially infected larvae of Anopheles kisumu with MU strains revealed that mosquitoes larvae are able to ingest and host MU during L1, L2, L3, and L4 developmental stages. However, we noticed an absence of these bacteria at both pupae and adult stages, certainly revealing the low ability of infected or colonized mosquitoes to vertically transmit MU to their offspring. Conclusion. The overall findings highlight the low implication of mosquitoes as biological vectors in the transmission cycle of MU from the risk environments to humans. |
| format | Article |
| id | doaj-art-80c089b1bffd46efbbccfd64dfc0f9ae |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-80c089b1bffd46efbbccfd64dfc0f9ae2025-02-03T01:21:28ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/13243101324310Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli UlcerRousseau Djouaka0Francis Zeukeng1Jude Daiga Bigoga2David N’golo Coulibaly3Genevieve Tchigossou4Romaric Akoton5Sylla Aboubacar6Sodjinin Jean-Eudes Tchebe7Clavella Nantcho Nguepdjo8Razack Adeoti9Innocent Djegbe10Manuele Tamo11Wilfred Fon Mbacham12Solange E. Kakou-Ngazoa13Anthony Ablordey14AgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninAgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninFaculty of Science, Department of Biochemistry, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonDepartment of Technics and Technology, Platform of Molecular Biology, Pasteur Institute Abidjan, P.O. Box 490 Abidjan 01, Abidjan, Côte d’IvoireAgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninAgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninDepartment of Technics and Technology, Platform of Molecular Biology, Pasteur Institute Abidjan, P.O. Box 490 Abidjan 01, Abidjan, Côte d’IvoireFaculty of Science and Techniques, University of Abomey-Calavi, P.O. Box 526, Abomey-Calavi, BeninFaculty of Science, Department of Biochemistry, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonAgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninAgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninAgroEcoHealth Platform, International Institute of Tropical Agriculture (IITA), 08 P.O. Box 0932, Tri-Postal, Cotonou, BeninFaculty of Science, Department of Biochemistry, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonDepartment of Technics and Technology, Platform of Molecular Biology, Pasteur Institute Abidjan, P.O. Box 490 Abidjan 01, Abidjan, Côte d’IvoireDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, P.O. Box 581, Legon, Accra, GhanaBackground. Buruli ulcer (BU) continues to be a serious public health threat in wet tropical regions and the mode of transmission of its etiological agent, Mycobacterium ulcerans (MU), remains poorly understood. In this study, mosquito species collected in endemic villages in Benin were screened for the presence of MU. In addition, the ability of mosquitoes larvae to pick up MU from their environment and remain colonized through the larval developmental stages to the adult stage was investigated. Methods. 7,218 adults and larvae mosquitoes were sampled from endemic and nonendemic villages and screened for MU DNA targets (IS2404, IS2606, and KR-B) using qPCR. Results. MU was not detected in any of the field collected samples. Additional studies of artificially infected larvae of Anopheles kisumu with MU strains revealed that mosquitoes larvae are able to ingest and host MU during L1, L2, L3, and L4 developmental stages. However, we noticed an absence of these bacteria at both pupae and adult stages, certainly revealing the low ability of infected or colonized mosquitoes to vertically transmit MU to their offspring. Conclusion. The overall findings highlight the low implication of mosquitoes as biological vectors in the transmission cycle of MU from the risk environments to humans.http://dx.doi.org/10.1155/2017/1324310 |
| spellingShingle | Rousseau Djouaka Francis Zeukeng Jude Daiga Bigoga David N’golo Coulibaly Genevieve Tchigossou Romaric Akoton Sylla Aboubacar Sodjinin Jean-Eudes Tchebe Clavella Nantcho Nguepdjo Razack Adeoti Innocent Djegbe Manuele Tamo Wilfred Fon Mbacham Solange E. Kakou-Ngazoa Anthony Ablordey Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer |
| title_full | Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer |
| title_fullStr | Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer |
| title_full_unstemmed | Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer |
| title_short | Evidences of the Low Implication of Mosquitoes in the Transmission of Mycobacterium ulcerans, the Causative Agent of Buruli Ulcer |
| title_sort | evidences of the low implication of mosquitoes in the transmission of mycobacterium ulcerans the causative agent of buruli ulcer |
| url | http://dx.doi.org/10.1155/2017/1324310 |
| work_keys_str_mv | AT rousseaudjouaka evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT franciszeukeng evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT judedaigabigoga evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT davidngolocoulibaly evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT genevievetchigossou evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT romaricakoton evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT syllaaboubacar evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT sodjininjeaneudestchebe evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT clavellanantchonguepdjo evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT razackadeoti evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT innocentdjegbe evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT manueletamo evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT wilfredfonmbacham evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT solangeekakoungazoa evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer AT anthonyablordey evidencesofthelowimplicationofmosquitoesinthetransmissionofmycobacteriumulceransthecausativeagentofburuliulcer |