Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury

Therapy with mesenchymal stem cells (MSCs) has showed to be promising due to its immunomodulatory function. Traumatic brain injury (TBI) triggers immune response and release of inflammatory mediators, mainly cytokines, by glial cells creating a hostile microenvironment for endogenous neural stem cel...

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Main Authors: Layla T. Galindo, Thais R. M. Filippo, Patricia Semedo, Carolina B. Ariza, Caroline M. Moreira, Niels O. S. Camara, Marimelia A. Porcionatto
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Neurology Research International
Online Access:http://dx.doi.org/10.1155/2011/564089
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author Layla T. Galindo
Thais R. M. Filippo
Patricia Semedo
Carolina B. Ariza
Caroline M. Moreira
Niels O. S. Camara
Marimelia A. Porcionatto
author_facet Layla T. Galindo
Thais R. M. Filippo
Patricia Semedo
Carolina B. Ariza
Caroline M. Moreira
Niels O. S. Camara
Marimelia A. Porcionatto
author_sort Layla T. Galindo
collection DOAJ
description Therapy with mesenchymal stem cells (MSCs) has showed to be promising due to its immunomodulatory function. Traumatic brain injury (TBI) triggers immune response and release of inflammatory mediators, mainly cytokines, by glial cells creating a hostile microenvironment for endogenous neural stem cells (NSCs). We investigated the effects of factors secreted by MSCs on NSC in vitro and analyzed cytokines expression in vitro in a TBI model. Our in vitro results show that MSC-secreted factors increase NSC proliferation and induce higher expression of GFAP, indicating a tendency toward differentiation into astrocytes. In vivo experiments showed that MSC injection at an acute model of brain injury diminishes a broad profile of cytokines in the tissue, suggesting that MSC-secreted factors may modulate the inflammation at the injury site, which may be of interest to the development of a favorable microenvironment for endogenous NSC and consequently to repair the injured tissue.
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institution Kabale University
issn 2090-1852
2090-1860
language English
publishDate 2011-01-01
publisher Wiley
record_format Article
series Neurology Research International
spelling doaj-art-80bd2bd3dcad4253a91d74978c85f71c2025-08-20T03:35:15ZengWileyNeurology Research International2090-18522090-18602011-01-01201110.1155/2011/564089564089Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain InjuryLayla T. Galindo0Thais R. M. Filippo1Patricia Semedo2Carolina B. Ariza3Caroline M. Moreira4Niels O. S. Camara5Marimelia A. Porcionatto6Departamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, BrazilDepartamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, BrazilDepartamento de Imunologia, Universidade de São Paulo, 05508-000 São Paulo, SP, BrazilDepartamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, BrazilDepartamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, BrazilDepartamento de Imunologia, Universidade de São Paulo, 05508-000 São Paulo, SP, BrazilDepartamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, SP, BrazilTherapy with mesenchymal stem cells (MSCs) has showed to be promising due to its immunomodulatory function. Traumatic brain injury (TBI) triggers immune response and release of inflammatory mediators, mainly cytokines, by glial cells creating a hostile microenvironment for endogenous neural stem cells (NSCs). We investigated the effects of factors secreted by MSCs on NSC in vitro and analyzed cytokines expression in vitro in a TBI model. Our in vitro results show that MSC-secreted factors increase NSC proliferation and induce higher expression of GFAP, indicating a tendency toward differentiation into astrocytes. In vivo experiments showed that MSC injection at an acute model of brain injury diminishes a broad profile of cytokines in the tissue, suggesting that MSC-secreted factors may modulate the inflammation at the injury site, which may be of interest to the development of a favorable microenvironment for endogenous NSC and consequently to repair the injured tissue.http://dx.doi.org/10.1155/2011/564089
spellingShingle Layla T. Galindo
Thais R. M. Filippo
Patricia Semedo
Carolina B. Ariza
Caroline M. Moreira
Niels O. S. Camara
Marimelia A. Porcionatto
Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury
Neurology Research International
title Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury
title_full Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury
title_fullStr Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury
title_full_unstemmed Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury
title_short Mesenchymal Stem Cell Therapy Modulates the Inflammatory Response in Experimental Traumatic Brain Injury
title_sort mesenchymal stem cell therapy modulates the inflammatory response in experimental traumatic brain injury
url http://dx.doi.org/10.1155/2011/564089
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