Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance
During the last 20 years, there has been increasing concern about inefficient vector control efforts due to insecticide resistance. A common mechanism causing insecticide resistance is mutational changes in the voltage-gated sodium channel, deemed knockdown resistance (<i>kdr</i>), resul...
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2025-05-01
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| author | Bianca M. Wimmer Cynthia Reinoso Webb Steven M. Presley |
| author_facet | Bianca M. Wimmer Cynthia Reinoso Webb Steven M. Presley |
| author_sort | Bianca M. Wimmer |
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| description | During the last 20 years, there has been increasing concern about inefficient vector control efforts due to insecticide resistance. A common mechanism causing insecticide resistance is mutational changes in the voltage-gated sodium channel, deemed knockdown resistance (<i>kdr</i>), resulting from continued pyrethroid application. Although closely related, there have been documented <i>kdr</i> differences and frequencies between <i>Aedes aegypti</i> and <i>Aedes albopictus</i>. Individual <i>Ae. aegypti</i> and <i>Ae. albopictus</i> from five counties in Texas, USA were tested using four single nucleotide polymorphisms genotyping assays to assess the <i>kdr</i> (F1534C, V1016I, V410L, and S989P) differences between the two species. Each mutation was analyzed independently by calculating frequencies and analyzing the difference using a Wilcox Rank Sum test. Significant differences were observed between <i>Ae. aegypti</i> and <i>Ae. albopictus</i> when comparing F1534C and V410L (<i>p</i>-value < 0.0001). Knockdown resistant mutation V1016I was not different between the two species. Individuals from both species had di-locus mutations, and individuals from <i>Ae. aegypti</i> had tri-locus mutations detected in combinations that have been reported to influence insecticide resistance. Given our findings, one can speculate that populations of both species are resistant to pyrethroids, thus likely limiting the success of control methods. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-05-01 |
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| series | Insects |
| spelling | doaj-art-80b70ad5d4e44720af08fd6eb61a1c692025-08-20T03:27:40ZengMDPI AGInsects2075-44502025-05-0116655110.3390/insects16060551Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide ResistanceBianca M. Wimmer0Cynthia Reinoso Webb1Steven M. Presley2Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79409, USADepartment of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79409, USADepartment of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79409, USADuring the last 20 years, there has been increasing concern about inefficient vector control efforts due to insecticide resistance. A common mechanism causing insecticide resistance is mutational changes in the voltage-gated sodium channel, deemed knockdown resistance (<i>kdr</i>), resulting from continued pyrethroid application. Although closely related, there have been documented <i>kdr</i> differences and frequencies between <i>Aedes aegypti</i> and <i>Aedes albopictus</i>. Individual <i>Ae. aegypti</i> and <i>Ae. albopictus</i> from five counties in Texas, USA were tested using four single nucleotide polymorphisms genotyping assays to assess the <i>kdr</i> (F1534C, V1016I, V410L, and S989P) differences between the two species. Each mutation was analyzed independently by calculating frequencies and analyzing the difference using a Wilcox Rank Sum test. Significant differences were observed between <i>Ae. aegypti</i> and <i>Ae. albopictus</i> when comparing F1534C and V410L (<i>p</i>-value < 0.0001). Knockdown resistant mutation V1016I was not different between the two species. Individuals from both species had di-locus mutations, and individuals from <i>Ae. aegypti</i> had tri-locus mutations detected in combinations that have been reported to influence insecticide resistance. Given our findings, one can speculate that populations of both species are resistant to pyrethroids, thus likely limiting the success of control methods.https://www.mdpi.com/2075-4450/16/6/551<i>Aedes aegypti</i><i>Aedes albopictus</i><i>kdr</i>mosquito controlinsecticide resistanceco-occurring mutations |
| spellingShingle | Bianca M. Wimmer Cynthia Reinoso Webb Steven M. Presley Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance Insects <i>Aedes aegypti</i> <i>Aedes albopictus</i> <i>kdr</i> mosquito control insecticide resistance co-occurring mutations |
| title | Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance |
| title_full | Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance |
| title_fullStr | Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance |
| title_full_unstemmed | Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance |
| title_short | Detection of Di- and Tri-Locus <i>kdr</i> Mutations in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> from Texas, USA, and the Implications for Insecticide Resistance |
| title_sort | detection of di and tri locus i kdr i mutations in i aedes aegypti i and i aedes albopictus i from texas usa and the implications for insecticide resistance |
| topic | <i>Aedes aegypti</i> <i>Aedes albopictus</i> <i>kdr</i> mosquito control insecticide resistance co-occurring mutations |
| url | https://www.mdpi.com/2075-4450/16/6/551 |
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