The influence of nonionic ammonia and mercury on the isozymes LDH and G6PDG of serum and anterior kidney from the immunized grass carp (Ctenopharyngodon idellus Cuvier et Valencinnes)
The grass carp were exposed to the water containing 0.5 mg/L NH<sub>3</sub> and 1.2 mg/L Hg<sup>2+</sup> for 2 weeks, then its serum and anterior kidney were used to the poly acrylamid gel electrophoresis for analyzing isozymes, lactate dehydrogenase (LDH) and glucose 6-phosp...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2000-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.05.0555 |
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| Summary: | The grass carp were exposed to the water containing 0.5 mg/L NH<sub>3</sub> and 1.2 mg/L Hg<sup>2+</sup> for 2 weeks, then its serum and anterior kidney were used to the poly acrylamid gel electrophoresis for analyzing isozymes, lactate dehydrogenase (LDH) and glucose 6-phosphate dehydrogenase (G6PDH). The results showed that NH<sub>3</sub> effects were mainly in anterior kidney, which expressed the B gene mainly or had still five bands but the activity of bands was changed; and that the bands expressed fewer for G6PDH in both components. Hg<sup>2+</sup> effect on LDH was chiefly quantity increasing of LDH1 and LDH2 in anterior kidney, yet G6PDH bands were decreased from 12 to 8 in serum and 9 in anterior kidney respectively, which hinted the isozyme gene expression was inhibited to some extent. |
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| ISSN: | 1008-9209 2097-5155 |