Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice
Leishmaniasis, a significant health issue in tropical regions, is spreading due to the challenges in treatment and the absence of an effective vaccine. The development of an effective vaccine for Leishmania major is crucial. This study aimed to assess the protective effectiveness of “Leish21,” a DNA...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Vaccine: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590136225000270 |
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| author | Sama Rashidi Effat Noori Bahram Kazemi Seyyed Javad Seyyed Tabaei Gholamreza Hatam |
| author_facet | Sama Rashidi Effat Noori Bahram Kazemi Seyyed Javad Seyyed Tabaei Gholamreza Hatam |
| author_sort | Sama Rashidi |
| collection | DOAJ |
| description | Leishmaniasis, a significant health issue in tropical regions, is spreading due to the challenges in treatment and the absence of an effective vaccine. The development of an effective vaccine for Leishmania major is crucial. This study aimed to assess the protective effectiveness of “Leish21,” a DNA vaccine containing multiple epitopes, against cutaneous leishmaniasis caused by L. major in different groups of BALB/c mice. The Leish21 vaccine was successfully transfected into eukaryotic cells, and its expression was confirmed using RT-PCR. Following infection with L. major promastigotes, immunized mice with Leish21 + IL12 and Leish21 showed a significant reduction in lesion diameter compared to the control group. In conclusion, the Leish21 vaccine triggered a Th1 immune response, and the addition of IL12 enhanced its efficacy against L. major infection. |
| format | Article |
| id | doaj-art-80b1d0ff47544118b6ff36c8cc1841c0 |
| institution | OA Journals |
| issn | 2590-1362 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Vaccine: X |
| spelling | doaj-art-80b1d0ff47544118b6ff36c8cc1841c02025-08-20T02:05:08ZengElsevierVaccine: X2590-13622025-06-012410063310.1016/j.jvacx.2025.100633Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c miceSama Rashidi0Effat Noori1Bahram Kazemi2Seyyed Javad Seyyed Tabaei3Gholamreza Hatam4Basic Sciences in infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranShahid Beheshti University of Medical Sciences Research Institute for Endocrine Sciences, Tehran, IranShahid Beheshti University, Tehran, IranShahid Beheshti University of Medical Sciences Research Institute for Endocrine Sciences, Tehran, IranBasic Sciences in infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author.Leishmaniasis, a significant health issue in tropical regions, is spreading due to the challenges in treatment and the absence of an effective vaccine. The development of an effective vaccine for Leishmania major is crucial. This study aimed to assess the protective effectiveness of “Leish21,” a DNA vaccine containing multiple epitopes, against cutaneous leishmaniasis caused by L. major in different groups of BALB/c mice. The Leish21 vaccine was successfully transfected into eukaryotic cells, and its expression was confirmed using RT-PCR. Following infection with L. major promastigotes, immunized mice with Leish21 + IL12 and Leish21 showed a significant reduction in lesion diameter compared to the control group. In conclusion, the Leish21 vaccine triggered a Th1 immune response, and the addition of IL12 enhanced its efficacy against L. major infection.http://www.sciencedirect.com/science/article/pii/S2590136225000270DNA vaccineLeishmania majorRecombinant plasmid, Leish21 |
| spellingShingle | Sama Rashidi Effat Noori Bahram Kazemi Seyyed Javad Seyyed Tabaei Gholamreza Hatam Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice Vaccine: X DNA vaccine Leishmania major Recombinant plasmid, Leish21 |
| title | Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice |
| title_full | Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice |
| title_fullStr | Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice |
| title_full_unstemmed | Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice |
| title_short | Exploring a promising vaccine candidate against Leishmania major: Insights from Gp63, LACK, TSA, LmSTI1and KMP11 antigens in BALB/c mice |
| title_sort | exploring a promising vaccine candidate against leishmania major insights from gp63 lack tsa lmsti1and kmp11 antigens in balb c mice |
| topic | DNA vaccine Leishmania major Recombinant plasmid, Leish21 |
| url | http://www.sciencedirect.com/science/article/pii/S2590136225000270 |
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