Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies
The interest in the production of lactic acid has increased due to its wide range of applications. In the present study, the variables that affect fermentative D(−) lactic acid production were investigated: neutralizing agents, pH, temperature, inoculum percentage, agitation, and concentration of th...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/4851612 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849410151968145408 |
|---|---|
| author | Susan Michelz Beitel Luciana Fontes Coelho Daiane Cristina Sass Jonas Contiero |
| author_facet | Susan Michelz Beitel Luciana Fontes Coelho Daiane Cristina Sass Jonas Contiero |
| author_sort | Susan Michelz Beitel |
| collection | DOAJ |
| description | The interest in the production of lactic acid has increased due to its wide range of applications. In the present study, the variables that affect fermentative D(−) lactic acid production were investigated: neutralizing agents, pH, temperature, inoculum percentage, agitation, and concentration of the medium components. An experimental design was applied to determine the optimal concentrations of the medium components and fermentation was studied using different feeding strategies. High production (122.41 g/L) and productivity (3.65 g/L·h) were efficiently achieved by Sporolactobacillus nakayamae in 54 h using a multipulse fed-batch technique with an initial medium containing 35 g/L of yeast extract (byproduct of alcohol production), 60 g/L of crystallized sugar, and 7.5 mL/L of salts. The fermentation process was conducted at 35°C and pH 6.0 controlled by NaOH with a 20% volume of inoculum and agitation at 125 rpm. The production of a high optically pure concentration of D(−) lactic acid combined with an environmentally friendly NaOH-based process demonstrates that S. nakayamae is a promising strain for D(−) lactic acid production. |
| format | Article |
| id | doaj-art-80afae9ee3904f84bc54473778ee315f |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-80afae9ee3904f84bc54473778ee315f2025-08-20T03:35:14ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/48516124851612Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch StrategiesSusan Michelz Beitel0Luciana Fontes Coelho1Daiane Cristina Sass2Jonas Contiero3Department of Biochemistry and Microbiology, Institute of Bioscience, São Paulo State University (UNESP), São Paulo, SP, BrazilDepartment of Biochemistry and Microbiology, Institute of Bioscience, São Paulo State University (UNESP), São Paulo, SP, BrazilDepartment of Biochemistry and Microbiology, Institute of Bioscience, São Paulo State University (UNESP), São Paulo, SP, BrazilDepartment of Biochemistry and Microbiology, Institute of Bioscience, São Paulo State University (UNESP), São Paulo, SP, BrazilThe interest in the production of lactic acid has increased due to its wide range of applications. In the present study, the variables that affect fermentative D(−) lactic acid production were investigated: neutralizing agents, pH, temperature, inoculum percentage, agitation, and concentration of the medium components. An experimental design was applied to determine the optimal concentrations of the medium components and fermentation was studied using different feeding strategies. High production (122.41 g/L) and productivity (3.65 g/L·h) were efficiently achieved by Sporolactobacillus nakayamae in 54 h using a multipulse fed-batch technique with an initial medium containing 35 g/L of yeast extract (byproduct of alcohol production), 60 g/L of crystallized sugar, and 7.5 mL/L of salts. The fermentation process was conducted at 35°C and pH 6.0 controlled by NaOH with a 20% volume of inoculum and agitation at 125 rpm. The production of a high optically pure concentration of D(−) lactic acid combined with an environmentally friendly NaOH-based process demonstrates that S. nakayamae is a promising strain for D(−) lactic acid production.http://dx.doi.org/10.1155/2017/4851612 |
| spellingShingle | Susan Michelz Beitel Luciana Fontes Coelho Daiane Cristina Sass Jonas Contiero Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies International Journal of Microbiology |
| title | Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies |
| title_full | Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies |
| title_fullStr | Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies |
| title_full_unstemmed | Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies |
| title_short | Environmentally Friendly Production of D(−) Lactic Acid by Sporolactobacillus nakayamae: Investigation of Fermentation Parameters and Fed-Batch Strategies |
| title_sort | environmentally friendly production of d lactic acid by sporolactobacillus nakayamae investigation of fermentation parameters and fed batch strategies |
| url | http://dx.doi.org/10.1155/2017/4851612 |
| work_keys_str_mv | AT susanmichelzbeitel environmentallyfriendlyproductionofdlacticacidbysporolactobacillusnakayamaeinvestigationoffermentationparametersandfedbatchstrategies AT lucianafontescoelho environmentallyfriendlyproductionofdlacticacidbysporolactobacillusnakayamaeinvestigationoffermentationparametersandfedbatchstrategies AT daianecristinasass environmentallyfriendlyproductionofdlacticacidbysporolactobacillusnakayamaeinvestigationoffermentationparametersandfedbatchstrategies AT jonascontiero environmentallyfriendlyproductionofdlacticacidbysporolactobacillusnakayamaeinvestigationoffermentationparametersandfedbatchstrategies |