Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis
Innate lymphoid cells (ILCs) have roles in many diseases and immune pathways. To determine the roles of these cells in patients with rheumatoid arthritis (RA) and mice with collagen-induced arthritis (CIA), we measured ILC subsets using flow cytometry and multiplex immunofluorescence staining. Patie...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/1915068 |
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| author | Fengfan Yang Xing Luo Wenxiao Zhu Jia Li Zhaohui Zheng Ping Zhu |
| author_facet | Fengfan Yang Xing Luo Wenxiao Zhu Jia Li Zhaohui Zheng Ping Zhu |
| author_sort | Fengfan Yang |
| collection | DOAJ |
| description | Innate lymphoid cells (ILCs) have roles in many diseases and immune pathways. To determine the roles of these cells in patients with rheumatoid arthritis (RA) and mice with collagen-induced arthritis (CIA), we measured ILC subsets using flow cytometry and multiplex immunofluorescence staining. Patients with stable RA had greater proportions of ILC2s and decreased proportions of ILC1s and ILC3s (all p<0.05). The 28-joint disease activity (DAS28) score had positive correlations with the proportion of ILC1s and negative correlations with ILC2s (both p<0.05). ILC2s of patients with RA expressed more IL-4 than healthy controls (p<0.05). The proportions of ILC1s and ILC2s were greater in mice with CIA (both p<0.05), especially the ILC2s in mice without arthritis after immunization and had correlations with multiple inflammatory and anti-inflammatory cytokines. Multiplex immunofluorescence staining described the distribution of ILCs in spleen tissues. Our results indicate that dysregulation of ILCs occurs during the pathogenesis of RA and CIA. |
| format | Article |
| id | doaj-art-80a5fca2169d4c5291cd2cf9c50d8d39 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-80a5fca2169d4c5291cd2cf9c50d8d392025-02-03T05:49:15ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/19150681915068Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced ArthritisFengfan Yang0Xing Luo1Wenxiao Zhu2Jia Li3Zhaohui Zheng4Ping Zhu5Department of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi’an, 710032 Shaanxi, ChinaDepartment of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi’an, 710032 Shaanxi, ChinaDepartment of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi’an, 710032 Shaanxi, ChinaDepartment of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi’an, 710032 Shaanxi, ChinaDepartment of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi’an, 710032 Shaanxi, ChinaDepartment of Clinical Immunology, PLA Specialized Research Institute of Rheumatology & Immunology, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Road, Xi’an, 710032 Shaanxi, ChinaInnate lymphoid cells (ILCs) have roles in many diseases and immune pathways. To determine the roles of these cells in patients with rheumatoid arthritis (RA) and mice with collagen-induced arthritis (CIA), we measured ILC subsets using flow cytometry and multiplex immunofluorescence staining. Patients with stable RA had greater proportions of ILC2s and decreased proportions of ILC1s and ILC3s (all p<0.05). The 28-joint disease activity (DAS28) score had positive correlations with the proportion of ILC1s and negative correlations with ILC2s (both p<0.05). ILC2s of patients with RA expressed more IL-4 than healthy controls (p<0.05). The proportions of ILC1s and ILC2s were greater in mice with CIA (both p<0.05), especially the ILC2s in mice without arthritis after immunization and had correlations with multiple inflammatory and anti-inflammatory cytokines. Multiplex immunofluorescence staining described the distribution of ILCs in spleen tissues. Our results indicate that dysregulation of ILCs occurs during the pathogenesis of RA and CIA.http://dx.doi.org/10.1155/2021/1915068 |
| spellingShingle | Fengfan Yang Xing Luo Wenxiao Zhu Jia Li Zhaohui Zheng Ping Zhu Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis Mediators of Inflammation |
| title | Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis |
| title_full | Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis |
| title_fullStr | Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis |
| title_full_unstemmed | Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis |
| title_short | Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced Arthritis |
| title_sort | dysregulation of innate lymphoid cells in patients with active rheumatoid arthritis and mice with collagen induced arthritis |
| url | http://dx.doi.org/10.1155/2021/1915068 |
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