MicroRNA expression and their molecular targets in food allergies: a systematic review

IntroductionMicroRNAs (miRs) play an essential role in adaptive and innate immune systems by regulating the development of immune cells. However, detailed studies of miRs’ role in food allergies are scarce compared to those of other allergic or non- allergic diseases. This systematic review aims to...

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Main Authors: Tekan Singh Rana, Rishipal Rastrapal Bansode, Jenny Pakhrin Rana, Leonard L. Williams
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1524392/full
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author Tekan Singh Rana
Rishipal Rastrapal Bansode
Jenny Pakhrin Rana
Leonard L. Williams
author_facet Tekan Singh Rana
Rishipal Rastrapal Bansode
Jenny Pakhrin Rana
Leonard L. Williams
author_sort Tekan Singh Rana
collection DOAJ
description IntroductionMicroRNAs (miRs) play an essential role in adaptive and innate immune systems by regulating the development of immune cells. However, detailed studies of miRs’ role in food allergies are scarce compared to those of other allergic or non- allergic diseases. This systematic review aims to study miRs expression and its role in food allergies (FAs) and determine the signature miRs in FAs.MethodResearch articles published since 2015 were selected from various databases: Scopus, PubMed, ScienceDirect, and Web of Science. Randomized clinical trials, observational clinical studies, and in vivo studies were assessed via the Cochrane Risk of Bias 2 tool, the Newcastle-Ottawa scale, and SYRCLE method, respectively. The characteristics of the included studies, population characteristics, and experimental details were extracted, and the data were synthesized narratively.ResultMiRs expression had been investigated in the context of cow milk allergy (CMA) and peanut allergy (PA) through both in vivo studies and clinical trials. Clinical trials included allergies to multiple combined foods, individual foods (such as milk, peanut, and what), and drugs and venom, while in vivo studies were conducted on milk, egg, and peanut allergies. MiR-146a, miR- 155, and miR-30a-5p were common miRs between in vivo studies and clinical trials. Moreover, few miRs were commonly studied between different types of food allergies. In clinical trials, miR-143-3p was studied in peanut allergy and non-celiac wheat sensitivity (NCWS), and miR-155 was studied in CMA and egg allergy in in vivo studies. Furthermore, the same miRs varied on their molecular target and effect depending on the type of food allergy.DiscussionThe study on signature miRs and their molecular target determination for the therapeutic purpose of food allergy is in its initial stage. For individual food allergies, miRs determination via next-generation sequencing (NGS), their validation via polymerase chain reaction (PCR), and target molecule determination via RNA interference (RNAi) should be the focus of future studies in order to determine reliable signature miRs of food allergy.
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spelling doaj-art-80a2c79fd7f44be29f9fc2e47acfbbca2025-08-20T03:53:12ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15243921524392MicroRNA expression and their molecular targets in food allergies: a systematic reviewTekan Singh Rana0Rishipal Rastrapal Bansode1Jenny Pakhrin Rana2Leonard L. Williams3Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, Kannapolis, NC, United StatesCenter for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, Kannapolis, NC, United StatesDepartment of Biology, North Carolina Agricultural and Technical State University, Greensboro, NC, United StatesCenter for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, Kannapolis, NC, United StatesIntroductionMicroRNAs (miRs) play an essential role in adaptive and innate immune systems by regulating the development of immune cells. However, detailed studies of miRs’ role in food allergies are scarce compared to those of other allergic or non- allergic diseases. This systematic review aims to study miRs expression and its role in food allergies (FAs) and determine the signature miRs in FAs.MethodResearch articles published since 2015 were selected from various databases: Scopus, PubMed, ScienceDirect, and Web of Science. Randomized clinical trials, observational clinical studies, and in vivo studies were assessed via the Cochrane Risk of Bias 2 tool, the Newcastle-Ottawa scale, and SYRCLE method, respectively. The characteristics of the included studies, population characteristics, and experimental details were extracted, and the data were synthesized narratively.ResultMiRs expression had been investigated in the context of cow milk allergy (CMA) and peanut allergy (PA) through both in vivo studies and clinical trials. Clinical trials included allergies to multiple combined foods, individual foods (such as milk, peanut, and what), and drugs and venom, while in vivo studies were conducted on milk, egg, and peanut allergies. MiR-146a, miR- 155, and miR-30a-5p were common miRs between in vivo studies and clinical trials. Moreover, few miRs were commonly studied between different types of food allergies. In clinical trials, miR-143-3p was studied in peanut allergy and non-celiac wheat sensitivity (NCWS), and miR-155 was studied in CMA and egg allergy in in vivo studies. Furthermore, the same miRs varied on their molecular target and effect depending on the type of food allergy.DiscussionThe study on signature miRs and their molecular target determination for the therapeutic purpose of food allergy is in its initial stage. For individual food allergies, miRs determination via next-generation sequencing (NGS), their validation via polymerase chain reaction (PCR), and target molecule determination via RNA interference (RNAi) should be the focus of future studies in order to determine reliable signature miRs of food allergy.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1524392/fullfood allergymiRNARNAipeanut allergyCMABALB/c
spellingShingle Tekan Singh Rana
Rishipal Rastrapal Bansode
Jenny Pakhrin Rana
Leonard L. Williams
MicroRNA expression and their molecular targets in food allergies: a systematic review
Frontiers in Immunology
food allergy
miRNA
RNAi
peanut allergy
CMA
BALB/c
title MicroRNA expression and their molecular targets in food allergies: a systematic review
title_full MicroRNA expression and their molecular targets in food allergies: a systematic review
title_fullStr MicroRNA expression and their molecular targets in food allergies: a systematic review
title_full_unstemmed MicroRNA expression and their molecular targets in food allergies: a systematic review
title_short MicroRNA expression and their molecular targets in food allergies: a systematic review
title_sort microrna expression and their molecular targets in food allergies a systematic review
topic food allergy
miRNA
RNAi
peanut allergy
CMA
BALB/c
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1524392/full
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AT jennypakhrinrana micrornaexpressionandtheirmoleculartargetsinfoodallergiesasystematicreview
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