PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma

Abstract Head and neck squamous cell carcinoma (HNSCC) is a malignancy with poor prognosis and chemotherapy resistance. Here, protein arginine methyltransferase 1 (PRMT1) is identified as a key driver of carboplatin (CBP) resistance in HNSCC. Analyses of clinical samples, cell lines, patient‐derived...

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Main Authors: Shixian Liu, Wentao Zhang, Weiwei Liu, Zhao Ding, Ruijing Zhang, Yuefeng Han, Zihao Niu, Mengdie Zhang, Hui Li, Dapeng Li, Zixi Wang, Jie Peng, Yu Wu, Yanxun Han, Zihui Xie, Jing Wu, Liang Qin, Zhongdong Hu, Xu Chen, Yunlong Hu, Yehai Liu, Shiyin Ma, Xiaojun Zha
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202417460
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author Shixian Liu
Wentao Zhang
Weiwei Liu
Zhao Ding
Ruijing Zhang
Yuefeng Han
Zihao Niu
Mengdie Zhang
Hui Li
Dapeng Li
Zixi Wang
Jie Peng
Yu Wu
Yanxun Han
Zihui Xie
Jing Wu
Liang Qin
Zhongdong Hu
Xu Chen
Yunlong Hu
Yehai Liu
Shiyin Ma
Xiaojun Zha
author_facet Shixian Liu
Wentao Zhang
Weiwei Liu
Zhao Ding
Ruijing Zhang
Yuefeng Han
Zihao Niu
Mengdie Zhang
Hui Li
Dapeng Li
Zixi Wang
Jie Peng
Yu Wu
Yanxun Han
Zihui Xie
Jing Wu
Liang Qin
Zhongdong Hu
Xu Chen
Yunlong Hu
Yehai Liu
Shiyin Ma
Xiaojun Zha
author_sort Shixian Liu
collection DOAJ
description Abstract Head and neck squamous cell carcinoma (HNSCC) is a malignancy with poor prognosis and chemotherapy resistance. Here, protein arginine methyltransferase 1 (PRMT1) is identified as a key driver of carboplatin (CBP) resistance in HNSCC. Analyses of clinical samples, cell lines, patient‐derived organoids, and xenograft models reveal that PRMT1 promotes tumor growth and CBP resistance through a novel, methyltransferase‐independent mechanism. Conditional PRMT1 knockout suppresses tumorigenesis and enhances CBP sensitivity in vivo, highlighting its essential role in HNSCC progression. Mechanistically, PRMT1 recruits the SWI/SNF chromatin remodeling complex via direct interaction with SMARCC1, leading to the transcriptional activation of insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2), which enhances CBP resistance and tumor growth. Notably, this function is independent of PRMT1's enzymatic activity, distinguishing it from its well‐established roles in arginine methylation. Furthermore, pre‐B‐cell leukemia homeobox 2 (PBX2) is identified as an upstream transcriptional activator that binds the PRMT1 promoter, driving its overexpression and reinforcing this oncogenic network. Clinically, high PBX2, PRMT1, SMARCC1, and IGF2BP2 expression correlates with malignant progression and poor prognosis in HNSCC patients. This study uncovers a previously unrecognized non‐catalytic function of PRMT1 and highlights the PBX2‐PRMT1‐SWI/SNF‐IGF2BP2 axis as a potential therapeutic target for overcoming CBP resistance in HNSCC.
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spelling doaj-art-8098e978f8fe489988fb33c0ae37d8ec2025-08-20T03:44:51ZengWileyAdvanced Science2198-38442025-06-011222n/an/a10.1002/advs.202417460PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell CarcinomaShixian Liu0Wentao Zhang1Weiwei Liu2Zhao Ding3Ruijing Zhang4Yuefeng Han5Zihao Niu6Mengdie Zhang7Hui Li8Dapeng Li9Zixi Wang10Jie Peng11Yu Wu12Yanxun Han13Zihui Xie14Jing Wu15Liang Qin16Zhongdong Hu17Xu Chen18Yunlong Hu19Yehai Liu20Shiyin Ma21Xiaojun Zha22Department of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Bengbu Medical University Bengbu 233004 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Bengbu Medical University Bengbu 233004 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Bengbu Medical University Bengbu 233004 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Bengbu Medical University Bengbu 233004 ChinaDepartment of Otolaryngology Head and Neck Surgery and Scientific Research and Experiment Center The Affiliated Bozhou Hospital of Anhui Medical University Bozhou 236800 ChinaDepartment of Biochemistry & Molecular Biology School of Basic Medicine Anhui Medical University Hefei 230032 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Bengbu Medical University Bengbu 233004 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Urology Shanghai General Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200080 ChinaModern Research Center for Traditional Chinese Medicine Beijing Research Institute of Chinese Medicine Beijing University of Chinese Medicine Beijing 100029 ChinaDepartment of Biochemistry & Molecular Biology School of Basic Medicine Anhui Medical University Hefei 230032 ChinaDepartment of Otolaryngology Head and Neck Surgery Anhui NO.2 Provincial People's Hospital Hefei 230041 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Anhui Medical University Hefei 230022 ChinaDepartment of Otolaryngology Head & Neck Surgery The First Affiliated Hospital of Bengbu Medical University Bengbu 233004 ChinaDepartment of Biochemistry & Molecular Biology School of Basic Medicine Anhui Medical University Hefei 230032 ChinaAbstract Head and neck squamous cell carcinoma (HNSCC) is a malignancy with poor prognosis and chemotherapy resistance. Here, protein arginine methyltransferase 1 (PRMT1) is identified as a key driver of carboplatin (CBP) resistance in HNSCC. Analyses of clinical samples, cell lines, patient‐derived organoids, and xenograft models reveal that PRMT1 promotes tumor growth and CBP resistance through a novel, methyltransferase‐independent mechanism. Conditional PRMT1 knockout suppresses tumorigenesis and enhances CBP sensitivity in vivo, highlighting its essential role in HNSCC progression. Mechanistically, PRMT1 recruits the SWI/SNF chromatin remodeling complex via direct interaction with SMARCC1, leading to the transcriptional activation of insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2), which enhances CBP resistance and tumor growth. Notably, this function is independent of PRMT1's enzymatic activity, distinguishing it from its well‐established roles in arginine methylation. Furthermore, pre‐B‐cell leukemia homeobox 2 (PBX2) is identified as an upstream transcriptional activator that binds the PRMT1 promoter, driving its overexpression and reinforcing this oncogenic network. Clinically, high PBX2, PRMT1, SMARCC1, and IGF2BP2 expression correlates with malignant progression and poor prognosis in HNSCC patients. This study uncovers a previously unrecognized non‐catalytic function of PRMT1 and highlights the PBX2‐PRMT1‐SWI/SNF‐IGF2BP2 axis as a potential therapeutic target for overcoming CBP resistance in HNSCC.https://doi.org/10.1002/advs.202417460chemoresistanceHNSCCIGF2BP2PRMT1SWI/SNF Complex
spellingShingle Shixian Liu
Wentao Zhang
Weiwei Liu
Zhao Ding
Ruijing Zhang
Yuefeng Han
Zihao Niu
Mengdie Zhang
Hui Li
Dapeng Li
Zixi Wang
Jie Peng
Yu Wu
Yanxun Han
Zihui Xie
Jing Wu
Liang Qin
Zhongdong Hu
Xu Chen
Yunlong Hu
Yehai Liu
Shiyin Ma
Xiaojun Zha
PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma
Advanced Science
chemoresistance
HNSCC
IGF2BP2
PRMT1
SWI/SNF Complex
title PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma
title_full PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma
title_fullStr PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma
title_full_unstemmed PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma
title_short PRMT1‐Mediated SWI/SNF Complex Recruitment via SMARCC1 Drives IGF2BP2 Transcription to Enhance Carboplatin Resistance in Head and Neck Squamous Cell Carcinoma
title_sort prmt1 mediated swi snf complex recruitment via smarcc1 drives igf2bp2 transcription to enhance carboplatin resistance in head and neck squamous cell carcinoma
topic chemoresistance
HNSCC
IGF2BP2
PRMT1
SWI/SNF Complex
url https://doi.org/10.1002/advs.202417460
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