Comparative study of purification methods for influenza-specific IgY from chicken eggs
Chicken eggs are widely used for different purposes including as a source of specific immunoglobulin (IgY). Therefore, this study aimed to evaluate various purification methods for influenza-specific IgY from chicken egg yolk. Three laying hens were vaccinated with an influenza vaccine. Influenza-sp...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2025-09-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
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| author | W. P. Rachmawan M. E. Saputri A. Esfandiari R. D. Soejoedono O. N. Poetri B. F. Prasetyo |
| author_facet | W. P. Rachmawan M. E. Saputri A. Esfandiari R. D. Soejoedono O. N. Poetri B. F. Prasetyo |
| author_sort | W. P. Rachmawan |
| collection | DOAJ |
| description | Chicken eggs are widely used for different purposes including as a source of specific immunoglobulin (IgY). Therefore, this study aimed to evaluate various purification methods for influenza-specific IgY from chicken egg yolk. Three laying hens were vaccinated with an influenza vaccine. Influenza-specific IgY was purified from the chicken egg yolk using four different purification techniques in-cluding (1) water-soluble sodium chloride (WS-NaCl), (2) water-soluble polyethylene glycol (WS-PEG), (3) WS-PEG-ammonium sulfate (WS-PEG-AS) precipitation, and (4) a commercial IgY purifi-cation kit. These methods were compared in terms of protein concentration, IgY structure, and speci-ficity against the influenza virus. The results showed that all purification methods successfully ob-tained influenza-specific IgY, as confirmed by the agar gel precipitation (AGP) test against influenza antigen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the WS-PEG precipitation method was unable to purify the entire IgY structure, while the other three methods were successful. The highest protein concentration was obtained using the commercial kit (9.931±0.214 mg/mL), followed by the WS-NaCl method (6.944±0.979 mg/mL). Although the commercial kit yielded the highest protein concentration, it may not be cost-effective for large-scale IgY production. The WS-NaCl method appeared to be a promising and feasible option for generating large quantities of IgY from chicken eggs. |
| format | Article |
| id | doaj-art-808f45a23bd54ae896d3974713fd6d08 |
| institution | Kabale University |
| issn | 1311-1477 1313-3543 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj-art-808f45a23bd54ae896d3974713fd6d082025-08-20T03:28:40ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432025-09-0128341142310.15547/bjvm.2024-0067Comparative study of purification methods for influenza-specific IgY from chicken eggsW. P. Rachmawan0M. E. Saputri1A. Esfandiari2R. D. Soejoedono3O. N. Poetri 4B. F. Prasetyo5Study Program of Medical Microbiology, IPB Postgraduate School, IPB ¬University, Kampus IPB Dramaga, Bogor, West Java, IndonesiaStudy Program of Medical Microbiology, IPB Postgraduate School, IPB ¬University, Kampus IPB Dramaga, Bogor, West Java, IndonesiaSchool of Veterinary Medicine & Biomedical Science, IPB University, Jl Agatis, Kampus IPB Dramaga, Bogor, West Java, IndonesiaDivision of Medical Microbiology, School of Veterinary Medicine & Biomedical Science, IPB University, Jl Agatis, Kampus IPB Dramaga, Bogor, West Java, IndonesiaDivision of Medical Microbiology, School of Veterinary Medicine & Biomedical Science, IPB University, Jl Agatis, Kampus IPB Dramaga, Bogor, West Java, IndonesiaDivision of Pharmacy, School of Veterinary Medicine & Biomedical Science, IPB University, Jl Agatis, Kampus IPB Dramaga, Bogor, West Java, IndonesiaChicken eggs are widely used for different purposes including as a source of specific immunoglobulin (IgY). Therefore, this study aimed to evaluate various purification methods for influenza-specific IgY from chicken egg yolk. Three laying hens were vaccinated with an influenza vaccine. Influenza-specific IgY was purified from the chicken egg yolk using four different purification techniques in-cluding (1) water-soluble sodium chloride (WS-NaCl), (2) water-soluble polyethylene glycol (WS-PEG), (3) WS-PEG-ammonium sulfate (WS-PEG-AS) precipitation, and (4) a commercial IgY purifi-cation kit. These methods were compared in terms of protein concentration, IgY structure, and speci-ficity against the influenza virus. The results showed that all purification methods successfully ob-tained influenza-specific IgY, as confirmed by the agar gel precipitation (AGP) test against influenza antigen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the WS-PEG precipitation method was unable to purify the entire IgY structure, while the other three methods were successful. The highest protein concentration was obtained using the commercial kit (9.931±0.214 mg/mL), followed by the WS-NaCl method (6.944±0.979 mg/mL). Although the commercial kit yielded the highest protein concentration, it may not be cost-effective for large-scale IgY production. The WS-NaCl method appeared to be a promising and feasible option for generating large quantities of IgY from chicken eggs.chickenegg yolkigyinfluenzapurification methods |
| spellingShingle | W. P. Rachmawan M. E. Saputri A. Esfandiari R. D. Soejoedono O. N. Poetri B. F. Prasetyo Comparative study of purification methods for influenza-specific IgY from chicken eggs Bulgarian Journal of Veterinary Medicine chicken egg yolk igy influenza purification methods |
| title | Comparative study of purification methods for influenza-specific IgY from chicken eggs |
| title_full | Comparative study of purification methods for influenza-specific IgY from chicken eggs |
| title_fullStr | Comparative study of purification methods for influenza-specific IgY from chicken eggs |
| title_full_unstemmed | Comparative study of purification methods for influenza-specific IgY from chicken eggs |
| title_short | Comparative study of purification methods for influenza-specific IgY from chicken eggs |
| title_sort | comparative study of purification methods for influenza specific igy from chicken eggs |
| topic | chicken egg yolk igy influenza purification methods |
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