Antifungal activities of flower extracts of Inula britannica L. with different solvents
In order to understand the fungicidal activity of Inula britannica, ethanolic extract of its flowers was separated by different solvents and the activities of different solvent extracts against 13 plant pathogenic fungi were tested in vitro and in vivo. The results showed the extraction rates of pet...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.03.008 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850069810645303296 |
|---|---|
| author | ZHOU Lin JIAO Bin GAO Fei SONG Tian-you ZHANG Xing |
| author_facet | ZHOU Lin JIAO Bin GAO Fei SONG Tian-you ZHANG Xing |
| author_sort | ZHOU Lin |
| collection | DOAJ |
| description | In order to understand the fungicidal activity of Inula britannica, ethanolic extract of its flowers was separated by different solvents and the activities of different solvent extracts against 13 plant pathogenic fungi were tested in vitro and in vivo. The results showed the extraction rates of petroleum ether, chloroform, ethylacetate and n-butyl alcohol were 34.89%, 8.99%, 8.63% and 22.72%, respectively. Chloroform extract at the concentration of 1.0 mg·mL<sup>-1</sup> exhibited strongly fungicidal activities to mycelium growth of the investigated fungi, and the inhibition rates against Phytophthora capsici and Sclerotinia sclerotiorum were 73.77% and 66.25%; the inhibition rates of the ethylacetate extract and n-butyl alcohol extract against P. capsici and S. sclerotiorum were 67.69%, 62.12% and 56.07%, 51.75%, respectively; the inhibition rate of the petroleum ether extract against Botrytis cintrea was 58.70%. With the same application rate as above, the chloroform extract could inhibit the spore germination of Colletotrichum dematium, Fusarium oxysporum on the cotton, Colletotrichum gloeosporioides, Fusarium graminearum and Fusarium oxysporum on the capsicum, and the inhibition rates were 96.00%, 92.44%, 80.43%, 78.45% and 71.84%, respectively; the inhibition rates of ethylacetate extract against six investigated fungi were from 40.86% to 86.42%; spore germination was inhibited markedly in F. oxysporum by petroleum ether extract, and the inhibition rate was 82.52%. The protective and therapeutic efficacies of chloroform extract against B. cinerea were 57.65% and 50.60% (6 d) on the tomato treated at the concentration of 2.0 mg·mL<sup>-1</sup>, respectively, and its therapeutic efficacy wasn't significantly different with the control fungicide-pyrimethanil at 0.4 mg·mL<sup>-1</sup>. |
| format | Article |
| id | doaj-art-808e925d552543219089f2d0306005cd |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2010-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-808e925d552543219089f2d0306005cd2025-08-20T02:47:40ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552010-05-013628729210.3785/j.issn.1008-9209.2010.03.00810089209Antifungal activities of flower extracts of Inula britannica L. with different solventsZHOU LinJIAO BinGAO FeiSONG Tian-youZHANG XingIn order to understand the fungicidal activity of Inula britannica, ethanolic extract of its flowers was separated by different solvents and the activities of different solvent extracts against 13 plant pathogenic fungi were tested in vitro and in vivo. The results showed the extraction rates of petroleum ether, chloroform, ethylacetate and n-butyl alcohol were 34.89%, 8.99%, 8.63% and 22.72%, respectively. Chloroform extract at the concentration of 1.0 mg·mL<sup>-1</sup> exhibited strongly fungicidal activities to mycelium growth of the investigated fungi, and the inhibition rates against Phytophthora capsici and Sclerotinia sclerotiorum were 73.77% and 66.25%; the inhibition rates of the ethylacetate extract and n-butyl alcohol extract against P. capsici and S. sclerotiorum were 67.69%, 62.12% and 56.07%, 51.75%, respectively; the inhibition rate of the petroleum ether extract against Botrytis cintrea was 58.70%. With the same application rate as above, the chloroform extract could inhibit the spore germination of Colletotrichum dematium, Fusarium oxysporum on the cotton, Colletotrichum gloeosporioides, Fusarium graminearum and Fusarium oxysporum on the capsicum, and the inhibition rates were 96.00%, 92.44%, 80.43%, 78.45% and 71.84%, respectively; the inhibition rates of ethylacetate extract against six investigated fungi were from 40.86% to 86.42%; spore germination was inhibited markedly in F. oxysporum by petroleum ether extract, and the inhibition rate was 82.52%. The protective and therapeutic efficacies of chloroform extract against B. cinerea were 57.65% and 50.60% (6 d) on the tomato treated at the concentration of 2.0 mg·mL<sup>-1</sup>, respectively, and its therapeutic efficacy wasn't significantly different with the control fungicide-pyrimethanil at 0.4 mg·mL<sup>-1</sup>.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.03.008<italic>Inula britannica</italic> L.extractantifungal activitybotanical fungicide |
| spellingShingle | ZHOU Lin JIAO Bin GAO Fei SONG Tian-you ZHANG Xing Antifungal activities of flower extracts of Inula britannica L. with different solvents 浙江大学学报. 农业与生命科学版 <italic>Inula britannica</italic> L. extract antifungal activity botanical fungicide |
| title | Antifungal activities of flower extracts of Inula britannica L. with different solvents |
| title_full | Antifungal activities of flower extracts of Inula britannica L. with different solvents |
| title_fullStr | Antifungal activities of flower extracts of Inula britannica L. with different solvents |
| title_full_unstemmed | Antifungal activities of flower extracts of Inula britannica L. with different solvents |
| title_short | Antifungal activities of flower extracts of Inula britannica L. with different solvents |
| title_sort | antifungal activities of flower extracts of inula britannica l with different solvents |
| topic | <italic>Inula britannica</italic> L. extract antifungal activity botanical fungicide |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.03.008 |
| work_keys_str_mv | AT zhoulin antifungalactivitiesofflowerextractsofinulabritannicalwithdifferentsolvents AT jiaobin antifungalactivitiesofflowerextractsofinulabritannicalwithdifferentsolvents AT gaofei antifungalactivitiesofflowerextractsofinulabritannicalwithdifferentsolvents AT songtianyou antifungalactivitiesofflowerextractsofinulabritannicalwithdifferentsolvents AT zhangxing antifungalactivitiesofflowerextractsofinulabritannicalwithdifferentsolvents |