Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity
Abstract The different steps of alternative splicing (AS) in plants and its regulatory mechanisms have already been studied extensively. Its broader impact on cell identity, plant immunity‐related genes, and their study as a whole remains to be investigated. Using transgenic plants, we sorted 11 dif...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.70022 |
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| author | Teura Barff Ingrid Berenice Sanchez Carrillo Valeria Paola Parra Gutiérrez Mélodie B. Plourde David L. Joly Hugo Germain |
| author_facet | Teura Barff Ingrid Berenice Sanchez Carrillo Valeria Paola Parra Gutiérrez Mélodie B. Plourde David L. Joly Hugo Germain |
| author_sort | Teura Barff |
| collection | DOAJ |
| description | Abstract The different steps of alternative splicing (AS) in plants and its regulatory mechanisms have already been studied extensively. Its broader impact on cell identity, plant immunity‐related genes, and their study as a whole remains to be investigated. Using transgenic plants, we sorted 11 different Arabidopsis thaliana cell types ranging from root to aerial organs using fluorescence‐activated cell sorting. RNA‐seq data were analyzed with vast‐tools and enabled us to generate a high‐resolution AS landscape across multiple cell types, all collected through the same experimental procedure. The analysis of cell type‐specific gene expression and alternative splicing events highlights the importance of AS on transcription and AS regulation itself. AS is also shown to be tightly linked to cell identity. By using closely related cell types, we captured alternative splicing events involved in specific stages of plant development. The columella cells, among others, show intensified AS regulation and an interesting splicing profile, especially regarding immunity‐related genes. Overall, our analysis brings a valuable tool in the study of cell type identity, plant immunity, and AS. |
| format | Article |
| id | doaj-art-8084684af0f04d13b7877e06f1d082dc |
| institution | OA Journals |
| issn | 1940-3372 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj-art-8084684af0f04d13b7877e06f1d082dc2025-08-20T02:35:07ZengWileyThe Plant Genome1940-33722025-06-01182n/an/a10.1002/tpg2.70022Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunityTeura Barff0Ingrid Berenice Sanchez Carrillo1Valeria Paola Parra Gutiérrez2Mélodie B. Plourde3David L. Joly4Hugo Germain5Department of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale Université du Québec à Trois‐Rivières Trois‐Rivières Québec CanadaDepartment of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale Université du Québec à Trois‐Rivières Trois‐Rivières Québec CanadaDepartment of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale Université du Québec à Trois‐Rivières Trois‐Rivières Québec CanadaDepartment of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale Université du Québec à Trois‐Rivières Trois‐Rivières Québec CanadaDépartement de biologie Université de Moncton Moncton New Brunswick CanadaDepartment of Chemistry, Biochemistry and Physics and Groupe de Recherche en Biologie Végétale Université du Québec à Trois‐Rivières Trois‐Rivières Québec CanadaAbstract The different steps of alternative splicing (AS) in plants and its regulatory mechanisms have already been studied extensively. Its broader impact on cell identity, plant immunity‐related genes, and their study as a whole remains to be investigated. Using transgenic plants, we sorted 11 different Arabidopsis thaliana cell types ranging from root to aerial organs using fluorescence‐activated cell sorting. RNA‐seq data were analyzed with vast‐tools and enabled us to generate a high‐resolution AS landscape across multiple cell types, all collected through the same experimental procedure. The analysis of cell type‐specific gene expression and alternative splicing events highlights the importance of AS on transcription and AS regulation itself. AS is also shown to be tightly linked to cell identity. By using closely related cell types, we captured alternative splicing events involved in specific stages of plant development. The columella cells, among others, show intensified AS regulation and an interesting splicing profile, especially regarding immunity‐related genes. Overall, our analysis brings a valuable tool in the study of cell type identity, plant immunity, and AS.https://doi.org/10.1002/tpg2.70022 |
| spellingShingle | Teura Barff Ingrid Berenice Sanchez Carrillo Valeria Paola Parra Gutiérrez Mélodie B. Plourde David L. Joly Hugo Germain Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity The Plant Genome |
| title | Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity |
| title_full | Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity |
| title_fullStr | Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity |
| title_full_unstemmed | Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity |
| title_short | Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity |
| title_sort | comprehensive mapping of arabidopsis alternative splicing landscape reveals key insights into plant development and immunity |
| url | https://doi.org/10.1002/tpg2.70022 |
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